Investigation of in vitro biocompatibility of novel pentablock copolymers for gene delivery

Journal of Biomedical Materials Research - Part A

Volumn 81, Issue 1, 2007, Pages 24-39

  Agarwal, Ankit ^a   Unfer, Robert ^b   Mallapragada, Surya K ^a  

^a Iowa State University   (United States)

^b Iowa Cancer Research Foundation   (United States)

Author keywords

Block copolymers; Cationic; Cytotoxicity; Gene delivery; Temperature sensitive

Indexed keywords

BIOCOMPATIBILITY; CELL DEATH; CELL MEMBRANES; CYTOTOXICITY; DNA; GENE EXPRESSION; TUMORS;

GENE DELIVERY; PENTABLOCK COPOLYMERS; POLYPLEX GELS; TRANSFECTION EFFICIENCY;

BLOCK COPOLYMERS;

CELL DNA; COPOLYMER; LACTATE DEHYDROGENASE; LUCIFERASE; NITROGEN; PHOSPHATE; PLASMID DNA;

APOPTOSIS; AQUEOUS SOLUTION; ARTICLE; BIOCOMPATIBILITY; CANCER CELL CULTURE; CELL VIABILITY; CYTOLYSIS; CYTOTOXICITY; DIFFUSION; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; MICROSCOPY; MOLECULAR WEIGHT; MORPHOLOGY; NECROSIS; NONVIRAL GENE DELIVERY SYSTEM; OVARY CARCINOMA; SCORING SYSTEM; SQUAMOUS CELL CARCINOMA; TEMPERATURE SENSITIVITY; TRANSGENE;

CASPASE 3; CASPASE 7; CELL DEATH; CELL LINE, TUMOR; DNA; GENE TRANSFER TECHNIQUES; HUMANS; MATERIALS TESTING; METHACRYLATES; MICELLES; POLYETHYLENE GLYCOLS; POLYMERS; PROPYLENE GLYCOLS;

EID: 33947243862     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.30920     Document Type: Article

References (62)

1. Schmidt-Wolf GD, Schmidt-Wolf IGH. Non-viral and hybrid vectors in human gene therapy: An update. Trends Mol Med 2003;9:67-72.
2. Merdan T, Kopecek J, Kissel T. Prospects of cationic polymers in gene and oligonucleotide therapy against cancer. Adv Drug Deliv Rev 2002;54:715-758.
3. Brigger I, Dubernet C, Couvreur P. Nanoparticles in cancer therapy and diagnosis. Adv Drug Deliv Rev 2002;54:631-651.
4. Jeong B, Kim SW, Bae YH. Thermosensitive sol-gel reversible hydrogels. Adv Drug Deliv Rev 2002;54:37-51.
5. Kost J, Langer R. Responsive polymeric delivery systems. Adv Drug Deliv Rev 2001;46(1-3):125-148.
6. Lauffenburger DA, Schaffer DV. The matrix delivers. Nat Med 1999;5:733, 734.
7. Fischer D, Li Y, Ahlemeyer B, Krieglstein J, Kissel T. In vitro cytotoxicity testing of polycations: Influence of polymer structure on cell viability and hemolysis. Biomaterials 2003;24:1121-1131.
8. De Groot CJ, Van Luyn MJA, Van Dijk-Wolthuis WNE, Cadee JA, Plantinga JA, Otter WD, Hennink WE. In vitro biocompatibility of biodegradable dextran-based hydrogels tested with human fibroblasts. Biomaterials 2001;22:1197-1203.
9. Verma IM. A tumultuous year for gene therapy. Mol Ther 2000;2:415, 416.
10. Liu F, Huang L. Development of non-viral vectors for systemic gene delivery. J Controlled Release 2002;78(1-3):259-266.
11. Schatzlein AG. Non-viral vectors in cancer gene therapy: Principles and progress. Anti-Cancer Drugs 2001;12:275-304.
12. Gondsho A, Irie K, Susaki H, Iwasawa H, Okuno S, Sugawara T. Tissue-targetting ability of saccharide-poly(L-lysine) conjugates. Biol Pharma 1994;17:275-282.
13. Ferruti P, Knobloch S, Ranucci E, Gialnasi E, Duncan R. A novel chemical modification of poly-L-lysine reducing toxicity while preserving cationic properties. Proc Int Symp Control Rel Bioact Matter 1997;24:45, 46.
14. Zauner W, Brunner S, Buschle M, Ogris M, Wagner E. Differential behaviour of lipid based and polycation based gene transfer systems in transfecting primary human fibroblasts: A potential role of polylysine in nuclear transport. Biochim Biophys Acta Gen Subj 1999;1428:57-67.
15. Boussif O, Lezoualch F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethyleneimine. Proc Natl Acad Sci USA 1995;92:7297-7301.
16. Iwai M, Harada Y, Tanaka S, Muramatsu A, Mori T, Kashima K, Imanishi J, Mazda O. Polyethylenimine-mediated suicide gene transfer induces a therapeutic effect for hepatocellular carcinoma in vivo by using an Epstein-Barr virus-based plasmid vector. Biochem Biophys Res Commun 2002;291:48-54.
17. Godbey WT, Barry MA, Saggau P, Wu KK, Mikos AG. Poly (ethylenimine)- mediated transfection: A new paradigm for gene delivery. J Biomed Mater Res 2000;51:321-328.
18. Tang MX, Fedemann CT, Szoka FC Jr. In vitro gene delivery by degraded polyamidoamine dendrimers. Bioconjugate Chem 1996;7:703-714.
19. Richardson SCW, Kolbe HVJ, Duncan R. Potential of low molecular mass chitosan as a DNA delivery system: Biocompatibility, body distribution and ability to complex and protect DNA. Int J Pharmaceut 1999;178:231-243.
20. Erbacher P, Zou S, Bettinger T, Steffan AM, Remy JS. Chitosan-based vector/DNA complexes for gene delivery: Biophysical characteristics and transfection ability. Pharmaceut Res 1998;15:1332-1339.
21. Van De Wetering P, Schuurmans-Nieuwenbroek NME, van Streenbergen MJ, Crommelin DJA, Hennink WE. Co-polymer of 2-(dimethylamino)ethyl methacrylate with ethoxytriethylen glycol methacrylate or N-vinyl-pyrroliodone as gene transfer agents. J Controlled Release 2000;64:193-203.
22. Van De Wetering P, Cherng JY, Talsma H, Crommelin DJA, Hennik WE. (2-Dimethylamino)ethyl-methacrylate based copolymers as gene transfer agents. J Controlled Release 1998;53:145-153.
23. Brazeau GA, Attia S, Poxon S, Hughes JA. In vivo myotoxicity of selected cationic macromolecules used in non-viral gene delivery. Pharmaceut Res 1998;15:680-684.
24. Dash PR, Read ML, Barret LB, Wolfert MA, Seymour LW. Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery. Gene Ther 1999; 6:643-650.
25. Choksakulnimitr S, Masusa S, Tokda H, Takakura Y, Hashida M. In vitro cytotoxicity of macromolecules in different cell culture systems. J Controlled Release 1995;34:223-241.
26. Florea BI, Meaney C, Junginger HE, Borchard G. Transfection efficiency and toxicity of polyethylenimine in differentiated Calu-3 and nondifferentiated COS-1 cell cultures. AAPS PharmSci 2002;4:E12.
27. Hill IRC, Garnett MC, Bignotti F, Davis SS. In vitro cytotoxicity of poly(amidoamine)s: Relevance to DNA delivery. Biochim Biophys Acta Gen Subj 1999;1427:161-174.
28. Morgan DML, Larvin VL, Pearson JD. Biochemical characterisation of polycation-induced cytotoxicity to human vascular endothelial cells. J Cell Sci 1989;94:553-559.
29. Gebhart CL, Kabanov AV. Evaluation of polyplexes as gene transfer agents. J Controlled Release 2001;73(2/3):401-416.
30. Kean T, Roth S, Thanou M. Trimethylated chitosans as non-viral gene delivery vectors: Cytotoxicity and transfection efficiency. J Controlled Release 2005;103:643-653.
31. Fischer D, Bieber T, Li Y, Elsässer H-P, Kissel T. A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: Effect of molecular weight on transfection efficiency and cytotoxicity. Pharmaceut Res 1999; 16:1273-1279.
32. Van De Wetering P, Cherng J-Y, Talsma H, Hennink WE. Relation between transfection efficiency and cytotoxicity of poly(2-(dimethylamino)ethyl methacrylate)/plasmid complexes. J Controlled Release 1997;49:59-69.
33. Agarwal A, Unfer R, Mallapragada SK. Novel cationic pentablock copolymers as non-viral vectors for gene therapy. J Controlled Release 2005;103:245-258.
34. Anderson BC, Cox SM, Bloom PD, Sheares W, Mallapragada SK. Synthesis and characterization of diblock and gel forming pentablock copolymers of tertiary amine methacrylates, poly (ethylene glycol) and poly(propylene glycol). Macromolecules 2003;36:1670-1676.
35. Determan MD, Cox JP, Seifert S, Thiyagarajan P, Mallapragada SK. Synthesis and characterization of temperature and pH responsive pentablock copolymers. Polymer 2005;46:6933-6946.
36. Determan MD, Guo L, Thiyagarajan P, Mallapragada SK. Supramolecular self-assembly of multiblock copolymers in aqueous solution. Langmuir 2006;22:1469-1473.
37. Determan MD, Chieh-Tsung L, Thiyagarajan P, Mallapragada SK. Self-assembly of temperature and pH-responsive pentablock copolymers. Polym Mater: Sci Eng 2005;23:808.
38. Edmondson J. A rapid and simple MTT-based spectrophotometric assay for determining drug sensitivity in monolayer cultures. J Tissue Culture Methods 1988;11:15-17.
39. Guess WL, Rosenbluth SA, Schimdt B. Agar diffusion method for toxicity screening of plastics on cultured cell monolayers. J Pharm Sci 1965;54:1545-1547.
40. Koh J-Y, Wie MB, Gwag BJ, Sensi SL, Canzoniero LMT, Demaro J, Csernansky C, Choi DW. Staurosporine-induced neuronal apoptosis. Exp Neurol 1995;135:153-159.
41. Godbey WT, Wu KK, Mikos AG. Poly(ethylenimine) and its role in gene delivery. J Controlled Release 1999;60(2/3):149-160.
42. Miller LJ, Marx J. Apoptosis. Science 1998;281:1301.
43. Lincz LF. Deciphering the apoptotic pathway: All roads lead to death. Immunol Cell Biol 1998;76:1-19.
44. Green DR, Reed JC. Mitochondria and apoptosis. Science 1998; 281:1309-1312.
45. Leroy D, Schmid N, Behr J-P, Filhol O, Pares S, Garin J, Bourgarit JJ, Chambaz EM, Cochet C. Direct identification of a polyamine binding domain on the regulatory subunit of the protein kinase casein kinase 2 by photoaffinity labeling. J Biol Chem 1995;270: 17400-17406.
46. Arnold LJJ, Dagan A, Gutheil J, Kaplan NO. Antineoplastic activity of poly(L-lysine) with some ascites tumor cells. Proc Natl Acad Sci USA 1979;76:3246-3250.
47. Vepa S, Scribner WM, Natarajan V. Activation of endothelial cell phospholipase D by polycations. Am J Physiol Lung Cell Mol Physiol 1997;272 (4 Part 1):L608-L613.
48. Rihova B. Biocompatibility of biomaterials: Hemocompatibility, immunocompatiblity and biocompatibility of solid polymeric materials and soluble targetable polymeric carriers. Adv Drug Deliv Rev 1996;21:157-176.
49. Dekie L, Toncheva V, Dubruel P, Schacht EH, Barrett L, Seymour LW. Poly-glutamic acid derivatives as vectors for gene therapy. J Controlled Release 2000;65(1/2):187-202.
50. Ryser HJ. A membrane effect of basic polymers dependent on molecular size. Nature 1967;215:934-936.
51. Singh AK, Kasinath BS, Lewis EJ. Interaction of polycations with cell-surface negative charges of epithelial cells. Biochim Biophys Acta Prot Struct Mol Enzymol 1992;1120:337-342.
52. Morgan DML, Clover J, Pearson JD. Effects of synthetic polycations on leucine incorporation, lactate dehydrogenase release, and morphology of human umbilical vein endothelial cells. J Cell Sci 1988;91:231-238.
53. Batrakova EV, Miller DW, Li S, Alakhov VY, Kabanov AV, Elmquist WF. Pluronic P85 enhances the delivery of digoxin to the brain: In vitro and in vivo studies. J Pharmacol Exp Therapeut 2001;296:551-557.
54. Kostarelos K, Tadros TF, Luckham PF. Physical conjugation of (tri-) block copolymers to liposomes toward the construction of sterically stabilized vesicle systems. Langmuir 1999;15:369-376.
55. Melik-Nubarov NS, Pomaz OO, Dorodnych TY, Badun GA, Ksenofontov AL, Schemchukova OB, Arzhakov SA. Interaction of tumor and normal blood cells with ethylene oxide and propylene oxide block copolymers. FEBS Lett 1999;446:194-198.
56. Batrakova EV, Li S, Vinogradov SV, Alakhov VY, Miller DW, Kabanov AV. Mechanism of pluronic effect on P-glycoprotein efflux system in blood-brain barrier: Contributions of energy depletion and membrane fluidization. J Pharmacol Exp Therapeut 2001;299:483-493.
57. Hannig J, Zhang D, Canaday DJ, Beckett MA, Astumian RD, Weichselbaum RR, Lee RC. Surfactant sealing of membranes permeabilized by ionizing radiation. Radiat Res 2000;154:171-177.
58. Lee RC, Hannig J, Matthews KL, Myerov A, Chen C-T. Pharmaceutical therapies for sealing of permeabilized cell membranes in electrical injuries. Ann N Y Acad Sci 1999;888:266-273.
59. Agren MS. An amorphous hydrogel enhances epithelialisation of wounds. Acta Dermato-Venereol 1998;78:119-122.
60. Cao YL, Lach E, Kim TH, Rodriguez A, Arevalo CA, Vacanti CA. Tissue-engineered nipple reconstruction. Plast Reconstructive Surg 1998;102:2293-2298.
61. Kushibiki T, Tomoshige R, Fukunaka Y, Kakemi M, Tabata Y. In vivo release and gene expression of plasmid DNA by hydrogels of gelatin with different cationization extents. J Controlled Release 2003;90:207-216.
62. Luo D, Woodrow-Mumford K, Belcheva N, Saltzman WM. Controlled DNA delivery systems. Pharmaceut Res 1999;16: 1300-1308.