Non-viral vectors in cancer gene therapy: Principles and progress

Anti-Cancer Drugs

Volumn 12, Issue 4, 2001, Pages 275-304

  Schatzlein, A G ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Cancer; Gene therapy; Non viral vector

Indexed keywords

VIRUS VECTOR;

ANTINEOPLASTIC ACTIVITY; CANCER; CANCER THERAPY; GENE MUTATION; GENE THERAPY; HUMAN; PRIORITY JOURNAL; REVIEW; SUICIDE GENE;

ANIMALS; CLINICAL TRIALS; DRUG CARRIERS; DRUG DELIVERY SYSTEMS; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE TARGETING; GENE THERAPY; GENETIC VECTORS; HUMANS; LIPIDS; NEOPLASMS; PEPTIDES; POLYETHYLENEIMINE; PROTEIN TRANSPORT;

EID: 0034997403     PISSN: 09594973     EISSN: None     Source Type: Journal    
DOI: 10.1097/00001813-200104000-00001     Document Type: Review

References (263)

1. 1. Anderson WF. Human gene therapy. Nature 1998; 392: 25-30.
2. 2. Gene therapy clinical trials. J Gene Med 2000; URL: http://www.wiley.co.uk/genetherapy/clinical
3. 3. Verma IM, Somia N. Gene therapy - promises, problems and prospects. Nature 1997; 389: 239-42.
4. 4. Lehrman S. Virus treatment questioned after gene therapy death. Nature 1999; 517-8.
5. 5. Fox JL. Gene therapy safety issues come to fore. Nat Biotechnol 1999; 17: 1153.
6. 6. Hui KM, Chia TF. Eradication of tumor growth via biolistic transformation with allogeneic MHC genes. Gene Ther 1997; 4: 762-7.
7. 7. Sawamura D, Ina S, Itai K, et al. In vivo gene introduction into keratinocytes using jet injection. Gene Ther 1999; 6: 1785-7.
8. 8. Oshima Y, Sakamoto T, Yamanaka I, Nishi T, Ishibashi T, Inomata H. Targeted gene transfer to corneal endothelium in vivo by electric pulse. Gene Ther 1998; 5: 1347-54.
9. 9. Lauer U, Burgelt E, Squire Z, et al. Shock wave permeabilization as a new gene transfer method. Gene Ther 1997; 4: 710-5.
10. 10. Luo D, Saltzman WM. Synthetic DNA delivery systems. Nat Biotechnol 2000; 18: 33-7.
11. 11. Felgner PL, Barenholz Y, Behr JP, et al. Nomenclature for synthetic gene delivery systems. Hum Gene Ther 1997; 8: 511-2.
12. 12. Pope IM, Poston GJ, Kinsella AR. The role of the bystander effect in suicide gene therapy. Eur J Cancer 1997; 33: 1005-16.
13. 13. Touraine RL, Ishii-Morita H, Ramsey WJ, Blaese RM. The bystander effect in the HSVtk/ganciclovir system and its relationship to gap junctional communication. Gene Ther 1998; 5: 1705-11.
14. 14. Takayama K, Ueno H, Nakanishi Y, et al. Suppression of tumor angiogenesis and growth by gene transfer of a soluble form of vascular endothelial growth factor receptor into a remote organ. Cancer Res 2000; 60: 2169-77.
15. 15. Chen QR, Kumar D, Stass SA, Mixson AJ. Liposomes complexed to plasmids encoding angiostatin and endostatin inhibit breast cancer in nude mice. Cancer Res 1999; 59: 3308-12.
16. h1-like profile of intratumoural cytokine expression. Int J Cancer 1997; 71: 267-74.
17. 17. Marcusson EG, Yacyshyn BR, Shanahan Jr WR, Dean NM. Preclinical and clinical pharmacology of antisense oligonucleotides. Mol Biotechnol 1999; 12: 1-11.
18. 18. Turner PC. Ribozymes. Their design and use in cancer. Adv Exp Med Biol 2000; 465: 303-18.
19. 19. Suzuki T, Anderegg B, Ohkawa T, et al. Adenovirus-mediated ribozyme targeting of Her-2/neu inhibits in vivo growth of breast cancer cells. Gene Ther 2000; 7: 241-8.
20. 20. Cobaleda C, Sanchez-Garcia I. In vivo inhibition by a site-specific catalytic RNA subunit of RNAse P designed against the bcr-abl oncogenic products: a novel approach for cancer treatment. Blood 2000; 95: 731-7.
21. 21. Kasono K, Heike Y, Xiang J, et al. Tetracycline-induced expression of an anti-c-myb single-chain antibody and its inhibitory effect on proliferation of the human leukemia cell line k562. Cancer Gene Ther 2000; 7: 151-9.
22. 22. Cochet O, Kenigsberg M, Delumeau I, et al. Intracellular expression of an antibody fragment-neutralizing p21 ras promotes tumor regression. Cancer Res 1998; 58: 1170-6.
23. 23. Piche A, Rancourt C. A role for intracellular immunization in chemosensitization of tumor cells? Gene Ther 1999; 6: 1202-9.
24. 24. Nielsen LL, Maneval DC. P53 tumor suppressor gene therapy for cancer. Cancer Gene Ther 1998; 5: 52-63.
25. 25. Baselga J. New horizons: gene therapy for cancer. Anti-Cancer Drugs 1999; 10(suppl 1): S39-42.
26. 26. Xu L, Pirollo KF, Tang WH, Rait A, Chang EH. Transferrin-liposome-mediated systemic p53 gene therapy in combination with radiation results in regression of human head and neck cancer xenografts. Hum Gene Ther 1999; 10: 2941-52.
27. 27. Claudio PP, Howard CM, Pacilio C, et al. Mutations in the retinoblastoma-related gene Rb2/p130 in lung tumors and suppression of tumor growth in vivo by retrovirus-mediated gene transfer. Cancer Res 2000; 60: 372-82.
28. 28. Tait DL, Obermiller PS, Hatmaker AR, Redlin-Frazier S, Holt JT. Ovarian cancer BRCA1 gene therapy. Phase I and II trial differences in immune response and vector stability. Clin Cancer Res 1999; 5: 1708-14.
29. 29. Tai YT, Strobel T, Kufe D, Cannistra SA. In vivo cytotoxicity of ovarian cancer cells through tumor-selective expression of the bax gene. Cancer Res 1999; 59: 2121-6.
30. 30. Favrot M, Coll JL, Louis N, Negoescu A. Cell death and cancer: replacement of apoptotic genes and inactivation of death suppressor genes in therapy. Gene Ther 1998; 5: 728-39.
31. 31. Tuting T, Storkus WJ, Lotze MT. Gene-based strategies for the immunotherapy of cancer. J Mol Med 1997; 75: 478-91.
32. 32. Tuting T, Wilson CC, Martin DM, et al. DNA vaccines targeting dendritic cells for the immunotherapy of cancer. Adv Exp Med Biol 1998; 451: 295-304.
33. 33. Gomez-Navarro J, Curiel DT, Douglas JT. Gene therapy for cancer. Eur J Cancer 1999; 35: 867-85.
34. 34. Kouraklis G. Progress in cancer gene therapy. Acta Oncol 1999; 38: 675-83.
35. 35. Lukacs KV, Porter CD, Pardo OE, et al. In vivo transfer of bacterial marker genes results in differing levels of gene expression and tumor progression in immunocompetent and immunodeficient mice. Hum Gene Ther 1999; 10: 2373-9.
36. 36. Hui KM, Ang PT, Huang L, Tay SK. Phase I study of immunotherapy of cutaneous metastases of human carcinoma using allogeneic and xenogeneic MHC DNA-liposome complexes. Gene Ther 1997; 4: 783-90.
37. 37. Pasquini S, Deng H, Reddy S, Giles-Davis W, Ertl H. The effect of CpG sequences on the B cell response to a viral glycoprotein encoded by a plasmid vector. Gene Ther 1999; 6: 1448-55.
38. 38. Li S, Wu SP, Whitmore M, et al. Effect of immune response on gene transfer to the lung via systemic administration of cationic lipidic vectors. Am J Physiol 1999; 276: L796-804.
39. 39. Zelphati O, Liang X, Hobart P, Felgner PL. Gene chemistry: Functionally and conformationally intact fluorescent plasmid DNA. Hum Gene Ther 1999; 10: 15-24.
40. 40. Yew NS, Wang KX, Przybylska M, et al. Contribution of plasmid DNA to inflammation in the lung after administration of cationic lipid:PDNA complexes. Hum Gene Ther 1999; 10: 223-34.
41. 41. Tan Y, Li S, Pitt BR, Huang L. The inhibitory role of CpG immunostimulatory motifs in cationic lipid vector-mediated transgene expression in vivo. Hum Gene Ther 1999; 10: 2153-61.
42. 42. Whitmore M, Li S, Huang L. LPD lipopolyplex initiates a potent cytokine response and inhibits tumor growth. Gene Ther 1999; 6: 1867-75.
43. 43. Freytag SO, Rogulski KR, Paielli DL, Gilbert JD, Kim JH. A novel three-pronged approach to kill cancer cells selectively: concomitant viral, double suicide gene, and radiotherapy. Hum Gene Ther 1998; 9: 1323-33.
44. 44. Mandell RB, Mandell LZ, Link Jr CJ. Radioisotope concentrator gene therapy using the sodium/iodide symporter gene. Cancer Res 1999; 59: 661-8.
45. 131I meta-iodobenzylguanidine. Gene Ther 1999; 6: 1147-52.
46. 6-alkylguanine-DNA-alkyltransferase protects human epithelial and hematopoietic cells against chloroethylating agent toxicity. Hum Gene Ther 1999; 10: 301-10.
47. 6-alkylguanine-DNA-alkyltransferase for engineering resistance of haemopoietic progenitor cells to multiple chemotherapeutic agents. Gene Ther 1999; 6: 1489-93.
48. 48. Zhao JQ, Hoare SF, McFarlane R, et al. Cloning and characterization of human and mouse telomerase RNA gene promoter sequences. Oncogene 1998; 16: 1345-50.
49. 49. Dachs GU, Dougherty GJ, Stratford IJ, Chaplin DJ. Targeting gene therapy to cancer: a review. Oncol Res 1997; 9: 313-25.
50. 50. Connors TA. The choice of prodrugs for gene directed enzyme prodrug therapy of cancer. Gene Ther 1995; 2: 702-9.
51. 51. Niculescu-Duvaz I, Springer CJ. Gene-directed enzyme prodrug therapy: a review of enzyme/prodrug combinations. Expert Opin Invest Drugs 1997; 6: 685-703.
52. 52. Benedetti S, Dimeco F, Pollo B, et al. Limited efficacy of the HSV-tk/GCV system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene. Hum Gene Ther 1997; 8: 1345-53.
53. 53. Elshami AA, Saavedra A, Zhang H, et al. Gap junctions play a role in the 'bystander effect' of the herpes simplex virus thymidine kinase/ganciclovir system in vitro. Gene Ther 1996; 3: 85-92.
54. 54. Ishii-Morita H, Agbaria R, Mullen CA, et al. Mechanism of 'bystander effect' killing in the herpes simplex thymidine kinase gene therapy model of cancer treatment. Gene Ther 1997; 4: 244-51.
55. 55. Touraine RL, Vahanian N, Ramsey WJ, Blaese RM. Enhancement of the herpes simplex virus thymidine kinase/ganciclovir bystander effect and its antitumor efficacy in vivo by pharmacologic manipulation of gap junctions, Hum Gene Ther 1998; 9: 2385-91.
56. 56. Bridgewater JA, Knox RJ, Pitts JD, Collins MK, Springer CJ. The bystander effect of the nitroreductase/CB 1954 enzyme/prodrug system is due to a cell-permeable metabolite. Hum Gene Ther 1997; 8: 709-17.
57. 57. Park JY, Elshami AA, Amin K, Rizk N, Kaiser LR, Albelda SM. Retinoids augment the bystander effect in vitro and in vivo in herpes simplex virus thymidine kinase/ ganciclovir-mediated gene therapy. Gene Ther 1997; 4: 909-17.
58. 58. Ruch RJ. The role of gap junctional intercellular communication in neoplasia. Ann Clin Lab Sci 1994; 24: 216-31.
59. 59. Dilber MS, Phelan A, Aints A, et al. Intercellular delivery of thymidine kinase prodrug activating enzyme by the herpes simplex virus protein, VP22. Gene Ther 1999; 6: 12-21.
60. 60. Feldman AL, Restifo NP, Alexander HR, et al. Antiangiogenic gene therapy of cancer utilizing a recombinant adenovirus to elevate systemic endostatin levels in mice. Cancer Res 2000; 60: 1503-6.
61. 61. Wadman M. NIH under fire over gene-therapy trials. Nature 2000; 403: 237.
62. 62. Wolff JA, Malone RW, Williams P, et al. Direct gene transfer into mouse muscle in vivo. Science 1990; 247: 1465-8.
63. 63. Hickman MA, Malone RW, Lehmann-Bruinsma K, et al. Gene expression following direct injection of DNA into liver. Hum Gene Ther 1994; 5: 1477-83.
64. 64. Yang JP, Huang L. Direct gene transfer to mouse melanoma by intratumor injection of free DNA. Gene Ther 1996; 3: 542-8.
65. 65. Barry ME, Pinto-Gonzalez D, Orson FM, McKenzie GJ, Petry GR, Barry MA. Role of endogenous endonucleases and tissue site in transfection and CpG-mediated immune activation after naked DNA injection. Hum Gene Ther 1999; 10: 2461-80.
66. 66. Niven R, Pearlman R, Wedeking T, et al. Biodistribution of radiolabeled lipid-DNA complexes and DNA in mice. J Pharm Sci 1998; 87: 1292-9.
67. 67. Houk BE, Hochhaus G, Hughes JA. Kinetic modeling of plasmid DNA degradation in rat plasma. PharmSci 1999; 1: http://mc.pharmsci.org/manuscripts/Houk003/frame_ composed.html.
68. 68. Zhang G, Vargo D, Budker V, Armstrong N, Knechtle S, Wolff JA. Expression of naked plasmid DNA injected into the afferent and efferent vessels of rodent and dog livers. Hum Gene Ther 1997; 8: 1763-72.
69. 69. Liu F, Song Y, Liu D. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther 1999; 6: 1258-66.
70. 70. Zhang G, Budker V, Wolff JA. High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA. Hum Gene Ther 1999; 10: 1735-7.
71. 71. Hirano T, Fujimoto J, Ueki T, et al. Persistent gene expression in rat liver in vivo by repetitive transfections using hvj-liposome. Gene Ther 1998; 5: 459-64.
72. 72. Fraser R, Bowler LM, Day WA, Dobbs B, Johnson HD, Lee D. High perfusion pressure damages the sieving ability of sinusoidal endothelium in rat livers. Br J Exp Pathol 1980; 61: 222-8.
73. 73. Budker V, Zhang G, Danko I, Williams P, Wolff J. The efficient expression of intravascularly delivered DNA in rat muscle. Gene Ther 1998; 5: 272-6.
74. 74. Huebner S, Battersby BJ, Grimm R, Cevc G. Lipid-DNA complex formation: reorganization and rupture of lipid vesicles in the presence of DNA as observed by cryoelectron microscopy. Biophys J 1999; 76: 3158-66.
75. 75. Salditt T, Koltover I, Radler JO, Safinya CR, Two-dimensional smectic ordering of linear DNA chains in self-assembled DNA-cationic liposome mixtures. Phys Rev Lett 1997; 79: 2582-5.
76. 76. Radler JO, Koltover I, Salditt T, Safinya CR. Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes. Science 1997; 275: 810-4.
77. 77. Koltover I, Salditt T, Radler JO, Safinya CR. An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery. Science 1998; 281: 78-81.
78. 78. Koltover I, Salditt T, Safinya CR. Phase diagram, stability, and overcharging of lamellar cationic lipid-DNA self-assembled complexes. Biophys J 1999; 77: 915-24.
79. 79. Felgner PL, Gadek TR, Holm M, et al. Lipofection - a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci USA 1987; 84: 7413-7.
80. 80. Felgner PL, Ringold GM. Cationic liposome-mediated transfection. Nature 1989; 337: 387-8.
81. 81. Zabner J. Cationic lipids used in gene transfer. Adv Drug Del Rev 1997; 27: 17-28.
82. 82. Mahato RI, Rolland A, Tomlinson E. Cationic lipid-based gene delivery systems: pharmaceutical perspectives. Pharm Res 1997; 14: 853-9.
83. 83. Li S, Huang L. Nonviral gene therapy: promises and challenges. Gene Ther 2000; 7: 31-4.
84. 84. Lasic DD, Templeton NS. Liposomes in gene-therapy. Adv Drug Del Rev 1996; 20: 221-66.
85. 85. Gao X, Huang L. Cationic liposome-mediated gene transfer. Gene Ther 1995; 2: 710-22.
86. 86. Balasubramaníam RP, Bennett MJ, Aberle AM, Malone JG, Nantz MH, Malone RW. Structural and functional-analysis of cationic transfection lipids - the hydrophobic domain. Gene Ther 1996; 3: 163-72.
87. 87. Kwoh DY, Coffin CC, Lollo CP, et al. Stabilization of poly-L-lysine/DNA polyplexes for in vivo gene delivery to the liver. Biochim Biophys Acta 1999; 1444: 171-90.
88. 88. Lucas P, Milroy DA, Thomas BJ, Moss SH, Pouton CW. Pharmaceutical and biological properties of poly(amino acid)/DNA polyplexes. J Drug Target 1999; 7: 143-56.
89. 89. Plank C, Tang MX, Wolfe AR, Szoka Jr FC. Branched cationic peptides for gene delivery: role of type and number of cationic residues in formation and in vitro activity of DNA polyplexes. Hum Gene Ther 1999; 10: 319-32.
90. 90. Harbottle RP, Cooper RG, Hart SL, et al. An RGD-oligolysine peptide: a prototype construct for integrin-mediated gene delivery. Hum Gene Ther 1998; 9: 1037-47.
91. 91. Erbacher P, Roche AC, Monsigny M, Midoux P. The reduction of the positive charges of polylysine by partial gluconoylation increases the transfection efficiency of polylysine/DNA complexes. Biochim Biophys Acta 1997; 1324: 27-36.
92. 92. Wadhwa MS, Collard WT, Adami RC, McKenzie DL, Rice KG. Peptide-mediated gene delivery: influence of peptide structure on gene expression. Bioconjug Chem 1997; 8: 81-8.
93. 93. Brown MD, Schätzlein AG, Brownlie A, et al. Preliminary characterisation of novel amino acid based polymeric vesicles as gene delivery agents. Bioconjug Chem 2000; 11: 880-91.
94. 94. Tang MX, Szoka FC, The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther 1997; 4: 823-32.
95. 95. Dash PR, Read ML, Barrett LB, Wolfert MA, Seymour LW. Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery. Gene Ther 1999; 6: 643-50.
96. 96. Wolfert MA, Schacht EH, Toncheva V, Ulbrich K, Nazarova O, Seymour LW. Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers. Hum Gene Ther 1996; 7: 2123-33.
97. 97. Katayose S, Kataoka K. Water-soluble polyion complex associates of DNA and poly(ethylene glycol)-poly(L-lysine) block copolymer. Bioconjug Chem 1997; 8: 702-7.
98. 98. Katayose S, Kataoka K. Remarkable Increase in nuclease resistance of plasmid DNA through supramolecular assembly with poly(ethylene glycol)-poly(L-lysine) block copolymer. J Pharm Sci 1998; 87: 160-3.
99. 99. Toncheva V, Wolfert MA, Dash PR, et al. Novel vectors for gene delivery formed by self-assembly of DNA with poly(L-lysine) grafted with hydrophilic polymers. Biochim Biophys Acta 1998; 1380: 354-68.
100. 100. Robinson P, Hawtrey A, Ariatti M, Govender P, Jagganath D. Effect of polyethylene glycol conjugated to DNA-transfecting complexes targeted at the transferrin receptor of hela cells. Drug Del 1997; 4: 115-9.
101. 101. Choi YH, Liu F, Kim JS, Choi YK, Park JS, Kim SW. Polyethylene glycol-grafted poly-L-lysine as polymeric gene carrier. J Controll Rel 1998; 54: 39-48.
102. 102. Dash PR, Read ML, Fisher KD, et al. Decreased binding to proteins and cells of polymeric gene delivery vectors surface modified with a multivalent hydrophilic polymer and retargeting through attachment of transferrin. J Biol Chem 2000; 275: 3793-802.
103. 103. Zhou XH, Klibanov AL, Huang L. Lipophilic polylysines mediate efficient DNA transfection in mammalian-cells. Biochim Biophys Acta 1991; 1065: 814.
104. 104. Zhou XH, Huang L. DNA transfection mediated by cationic liposomes containing lipopolylysine - characterization and mechanism of action. Biochim Biophys Acta 1994; 1189: 195-203.
105. 105. Kim JS, Kim BI, Maruyama A, Akaike T, Kim SW. A new non-viral DNA delivery vector: the terplex system. J Control Rel 1998; 53: 175-82.
106. 106. Coll JL, Combaret V, Metchler K, et al. In vitro targeting and specific transfection of human neuroblastoma cells by CHCE7 antibody-mediated gene transfer. Gene Ther 1997; 4: 156-61.
107. 107. Shewring L, Collins L, Lightman SL, Hart S, Gustafsson K, Fabre JW. A nonviral vector system for efficient gene transfer to corneal endothelial cells via membrane integrin. Transplantation 1997; 64: 763-9.
108. 108. Pouton CW, Lucas P, Thomas BJ, Uduehi AN, Milroy DA, Moss SH. Polycation-DNA complexes for gene delivery: a comparison of the biopharmaceutical properties of cationic polypeptides and cationic lipids. J Control Rel 1998; 53: 289-99.
109. 109. Sosnowski BA, Gonzalez AM, Chandler LA, Buechler YJ, Pierce GF, Baird A. Targeting DNA to cells with basic fibroblast growth factor (FGF2). J Biol Chem 1996; 271: 33647-53.
110. 110. Curiel DT, Agarwal S, Wagner E, Cotten M. Adenovirus enhancement of transferrin polylysine-mediated gene delivery, Proc Natl Acad Sci USA 1991; 88: 8850-4.
111. 111. Wagner E, Zatloukal K, Cotten M, et al. Coupling of adenovirus to transferrin polylysine DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. Proc Natl Acad Sci USA 1992; 89: 6099-103.
112. 112. Wolfert MA, Seymour LW. Chloroquine and amphipathic peptide helices show synergistic transfection in vitro. Gene Ther 1998; 5: 409-14.
113. 113. Midoux P, Monsigny M. Efficient gene transfer by histidylated polylysine/pDNA complexes. Bioconjug Chem 1999; 10: 406-11.
114. 114. Wu GY, Wu CH. Receptor-mediated in vitro gene transformation by a soluble DNA carrier system. J Biol Chem 1987; 262: 4429-32.
115. 115. Wu CH, Wilson JM, Wu GY. Targeting genes: delivery and persistent expression of a foreign gene driven by mammalian regulatory elements in vivo. J Biol Chem 1989; 264: 169857.
116. 116. Cotten M, Langle-Rouault F, Kirlappos H, et al. Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels. Proc Natl Acad Sci USA 1990; 87: 4033-7.
117. 117. Mahato RI, Takemura S, Akamatsu K, Nishikawa M, Takakura Y, Hashida M. Physicochemical and disposition characteristics of antisense oligonucleotides complexed with glycosylated poly(L-lysine). Biochem Pharmacol 1997; 53: 887-95.
118. 118. Fajac I, Briand P, Monsigny M, Midoux P. Sugar-mediated uptake of glycosylated polylysines and gene transfer into normal and cystic fibrosis airway epithelial cells. Hum Gene Ther 1999; 10: 395-406.
119. 119. Ziady AG, Ferkol T, Gerken T, Dawson DV, Perlmutter DH, Davis PB. Ligand substitution of receptor targeted DNA complexes affects gene transfer into hepatoma cells. Gene Ther 1998; 5: 1685-97.
120. 120. Schaffer DV, Lauffenburger DA. Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery. J Biol Chem 1998; 273: 28004-9.
121. 121. Xu B, Wiehle S, Roth JA, Cristiano RJ. The contribution of poly-L-lysine, epidermal growth factor and streptavidin to EGF/pll/DNA polyplex formation. Gene Ther 1998; 5: 1235-43.
122. 122. Zauner W, Ogris M, Wagner E. Polylysine-based transfection systems utilizing receptor-mediated delivery. Adv Drug Del Rev 1998; 30: 97-113.
123. 123. Hong K, Zheng W, Baker A, Papahadjopoulos D. Stabilization of cationic liposome-plasmid DNA complexes by polyamines and poly(ethylene glycol)-phospholipid conjugates for efficient in vivo gene delivery. FEBS Letters 1997; 400: 233-7.
124. 124. Nishikawa M, Takemura S, Takakura Y, Hashida M. Targeted delivery of plasmid DNA to hepatocytes in vivo: optimization of the pharmacokinetics of plasmid DNA/galactosylated poly(L-lysine) complexes by controlling their physicochemical properties. J Pharmacol Exp Ther 1998; 287: 408-15.
125. 125. Hashida M, Takemura S, Nishikawa M, Takakura Y. Targeted delivery of plasmid DNA complexed with galactosylated poly(L-lysine). J Control Rel 1998; 53: 301-10.
126. 126. Hisayasu S, Miyauchi M, Akiyama K, Gotoh T, Satoh S, Shimada T. In vivo targeted gene transfer into liver cells mediated by a novel galactosyl-D-lysine/D-serine copolymer. Gene Ther 1999; 6: 689-93.
127. 127. Boussif O, Lezoualc'h F, Zanta MA, et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci USA 1995; 92: 7297-301.
128. 128. Suh J, Paik HJ, Hwang BK. Ionization of poly(ethylenimine) and poly(allylamine) at various pHs. Bioorg Chem 1994; 22: 318-27.
129. 129. Godbey W, Wu K, Hirasaki G, Mikos A. Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency. Gene Ther 1999; 6: 1380-8.
130. 130. Diebold SS, Kursa M, Wagner E, Cotten M, Zenke M. Mannose polyethylenimine conjugates for targeted DNA delivery into dendritic cells. J Biol Chem 1999; 274: 19087-94.
131. 131. Goula D, Benoist C, Mantero S, Merlo G, Levi G, Demeneix BA. Polyethylenimine-based intravenous delivery of transgenes to mouse lung. Gene Ther 1998; 5: 1291-5.
132. 132. Ogris M, Brunner S, Schuller S, Kircheis R, Wagner E. Pegylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther 1999; 6: 595-605.
133. 133. Li S, Tan Y, Viroonchatapan E, Pitt BR, Huang L. Targeted gene delivery to pulmonary endothelium by anti-PECAM antibody. Am J Physiol 2000; 278: L504-11.
134. 134. Plank C, Mechtler K, Szoka Jr FC, Wagner E. Activation of the complement system by synthetic DNA complexes: a potential barrier for intravenous gene delivery. Hum Gene Ther 1996; 7: 1437-46.
135. 135. Zanta MA, Boussif O, Adib A, Behr JP. In vitro gene delivery to hepatocytes with galactosylated polyethylenimine. Bioconjug Chem 1997; 8: 839-44.
136. 136. Bettinger T, Remy JS, Erbacher P. Size reduction of galactosylated PEI/DNA complexes improves lectin-mediated gene transfer into hepatocytes. Bioconjug Chem 1999; 10: 558-61.
137. 137. Diebold SS, Lehrmann H, Kursa M, Wagner E, Cotten M, Zenke M. Efficient gene delivery into human dendritic cells by adenovirus polyethylenimine and mannose polyethylenimine transfection. Hum Gene Ther 1999; 10: 775-86.
138. 138. Erbacher P, Remy JS, Behr JP. Gene transfer with synthetic virus-like particles via the integrin-mediated endocytosis pathway. Gene Ther 1999; 6: 138-45.
139. 139. Kircheis R, Kichler A, Wallner G, et al. Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery. Gene Ther 1997; 4: 409-18.
140. 140. Abdallah B, Hassan A, Benoist C, Goula D, Behr JP, Demeneix BA. A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain; polyethylenimine. Hum Gene Ther 1996; 7: 1947-54.
141. 141. Goula D, Remy JS, Erbacher P, et al. Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system. Gene Ther 1998; 5: 712-7.
142. 142. Lemkine GF, Goula D, Becker N, Paleari L, Levi G, Demeneix BA. Optimisation of polyethylenimine-based gene delivery to mouse brain. J Drug Target 1999; 7: 305-12.
143. 143. Boletta A, Benigni A, Lutz J, Remuzzi G, Soria MR, Monaco L. Nonviral gene delivery to the rat kidney with polyethylenimine. Hum Gene Ther 1997; 8: 1243-51.
144. 144. Ferrari S, Moro E, Pettenazzo A, Behr JP, Zacchello F, Scarpa M. Exgen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo. Gene Ther 1997; 4: 1100-6.
145. 145. Godbey WT, Wu KK, Mikos AG. Size matters: molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle. J Biomed Mater Res 1999; 45: 268-75.
146. 146. Bragonzi A, Boletta A, Biffi A, et al. Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs. Gene Ther 1999; 6: 1995-2004.
147. 147. Coll JL, Chollet P, Brambilla E, Desplanques D, Behr JP, Favrot M. In vivo delivery to tumors of DNA complexed with linear polyethylenimine. Hum Gene Ther 1999; 10: 1659-66.
148. 148. Goula D, Becker N, Lemkine GF, et al. Rapid crossing of the pulmonary endothelial barrier by polyethylenimine/ DNA complexes. Gene Ther 2000; 7: 499-504.
149. 149. Nguyen HK, Lemieux P, Vinogradov SV, et al. Evaluation of polyether-polyethyleneimine graft copolymers as gene transfer agents. Gene Ther 2000; 7: 126-38.
150. 150. Mahato RI. Non-viral peptide-based approaches to gene delivery. J Drug Target 1999; 7: 249-68.
151. 151. Wilke M, Fortunati E, Van DenBroek M, Hoogeveen AT, Scholte BJ. Efficacy of a peptide-based gene delivery system depends on mitotic activity. Gene Ther 1996; 3: 1133-42.
152. 152. Colin M, Harbottle RP, Knight A, et al. Liposomes enhance delivery and expression of an RGD-oligolysine gene transfer vector in human tracheal cells. Gene Ther 1998; 5: 1488-98.
153. 153. Murphy JE, Uno T, Hamer JD, Cohen FE, Dwarki V, Zuckermann RN. A combinatorial approach to the discovery of efficient cationic peptoid reagents for gene delivery. Proc Natl Acad Sci USA 1998; 95: 1517-22.
154. 154. Hart S, Collins L, Gustafsson K, Fabre J. Integrin-mediated transfection with peptides containing arginine-glycine-aspartic acid domains. Gene Ther 1997; 4: 1225-30.
155. 155. Huang CY, Uno T, Murphy JE, et al. Lipitoids-novel cationic lipids for cellular delivery of plasmid DNA in vitro. Chem Biol 1998; 5: 345-54.
156. 156. Schwartz B, Ivanov MA, Pitard B, et al. Synthetic DNA-compacting peptides derived from human sequence enhance cationic lipid-mediated gene transfer in vitro and in vivo. Gene Ther 1999; 6: 282-92.
157. 157. Mislick KA, Baldeschwieler JD. Evidence for the role of proteoglycans in cation-mediated gene transfer. Proc Natl Acad Sci USA 1996; 93: 12349-54.
158. 158. Yang JP, Huang L. Overcoming the inhibitory effect of serum on lipofection by increasing the charge ratio of cationic liposome to DNA. Gene Ther 1997; 4: 950-60.
159. 159. Li S, Rizzo MA, Bhattacharya S, Huang L. Characterization of cationic lipid-protamine-DNA (LPD) complexes for intravenous gene delivery. Gene Ther 1998; 5: 930-7.
160. 160. Crook K, Stevenson BJ, Dubouchet M, Porteous DJ. Inclusion of cholesterol in DOTAP transfection complexes increases the delivery of DNA to cells in vitro in the presence of serum. Gene Ther 1998; 5: 137-43.
161. 161. Semple SC, Chonn A, Cullis PR. Influence of cholesterol on the association of plasma proteins with liposomes. Biochemistry 1996; 35: 2521-5.
162. 162. Oja CD, Semple SC, Chonn A, Cullis PR. Influence of dose on liposome clearance: critical role of blood proteins. Biochim Biophys Acta 1996; 1281: 31-7.
163. 163. Zelphati O, Uyechi LS, Barron LG, Szoka Jr FC. Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells. Biochim Biophys Acta 1998; 1390: 119-33.
164. 164. Wang J, Guo X, Xu Y, Barron L, Szoka Jr FC. Synthesis and characterization of long chain alkyl acyl carnitine esters. Potentially biodegradable cationic lipids for use in gene delivery. J Med Chem 1998; 41: 2207-15.
165. 165. Oupicky D, Konak C, Dash PR, Seymour LW, Ulbrich K. Effect of albumin and polyanion on the structure of DNA complexes with polycation containing hydrophillc nonionic block. Bioconjug Chem 1999; 10: 764-72.
166. 166. Yang JP, Huang L. Time-dependent maturation of cationic liposome-DNA complex for serum resistance. Gene Ther 1998; 5: 380-7.
167. 167. Kikuchi A, Aoki Y, Sugaya S, et al. Development of novel cationic liposomes for efficient gene transfer into peritoneal disseminated tumor. Hum Gene Ther 1999; 10: 947-55.
168. 168. McLean JW, Fox EA, Baluk P, et al. Organ-specific endothelial cell uptake of cationic liposome-DNA complexes in mice. Am J Physiol Heart Circ Physiol 1997; 42: H387-404.
169. 169. Jain RK. Delivery of molecular and cellular medicine to solid tumors. Adv Drug Del Rev 1997; 26: 71-90.
170. 170. Hashizume H, Baluk P, Morikawa S, et al. Openings between defective endothelial cells explain tumor vessel leakiness. Am J Pathol 2000; 156: 1363-80.
171. 171. Griesenbach U, Chonn A, Cassady R, et al. Comparison between intratracheal and intravenous administration of liposome-DNA complexes for cystic fibrosis lung gene therapy. Gene Ther 1998; 5: 181-8.
172. 172. Liu F, Qi H, Huang L, Liu D. Factors controlling the efficiency of cationic lipidmediated transfection in vivo via intravenous administration. Gene Ther 1997; 4: 517-23.
173. 173. Hofland HE, Nagy D, Liu JJ, et al. In vivo gene transfer by intravenous administration of stable cationic lipid/ DNA complex. Pharm Res 1997; 14: 742-9.
174. 174. Zhu N, Liggitt D, Liu Y, Debs R. Systemic gene expression after intravenous DNA delivery into adult mice. Science 1993; 261: 209-11.
175. 175. Maeda H. The tumor blood vessel as an ideal target for macromolecular anticancer agents. J Control Rel 1992; 19: 315-24.
176. 176. Yuan F, Leunig M, Huang SK, Berk DA, Papahadjopoulos D, Jain RK. Microvascular permeability and interstitial penetration of sterically stabilized (stealth) liposomes in a human tumor xenograft. Cancer Res 1994; 54: 3352-6.
177. 177. Gabizon A, Isacson R, Libson E, et al. Clinical-studies of liposome-encapsulated doxorubicin. Acta Oncol 1994; 33: 779-786.
178. 178. Vasey PA, Kaye SB, Morrison R, et al. Phase I clinical and pharmacokinetic study of PK I [n-(2-hydroxypropyl)-methacrylamide copolymer doxorubicin]: first member of a new class of chemotherapeutic agents-drug-polymer conjugates. Cancer Research Campaign Phase I/II Committee. Clin Cancer Res 1999; 5: 83-94.
179. 179. Monsky WL, Fukumura D, Gohongi T, et al. Augmentation of transvascular transport of macromolecules and nanoparticles in tumors using vascular endothelial growth factor. Cancer Res 1999; 59: 4129-35.
180. 180. Thurston G, McLean JW, Rizen M, et al. Cationic liposomes target angiogenic endothelial cells in tumors and chronic inflammation in mice. J Clin Invest 1998; 101: 1401-13.
181. 33P]DNA following intravenous administration of DNA/cationic lipid complexes in mice: use of a novel radionuclide approach. J Pharm Sci 1996; 85: 612-8.
182. 182. Song YK, Liu DX. Free liposomes enhance the transfection activity of DNA/lipid complexes in vivo by intravenous administration. Biochim Biophys Acta 1998; 1372: 141-50.
183. 183. Song YK, Liu F, Liu D. Enhanced gene expression in mouse lung by prolonging the retention time of intravenously injected plasmid DNA. Gene Ther 1998; 5: 1531-7.
184. 184. Barron LG, Meyer KB, Szoka FC. Effects of complement depletion on the pharmacokinetics and gene delivery mediated by cationic lipid DNA complexes. Hum Gene Ther 1998; 9: 315-23.
185. 185. Hillery E, Cheng S, Geddes D, Alton E. Effects of altering dosing on cationic liposome-mediated gene transfer to the respiratory epithelium. Gene Ther 1999; 6: 1313-6.
186. 186. Barron LG, Gagne L, Szoka Jr FC. Lipoplex-mediated gene delivery to the lung occurs within 60 minutes of intravenous administration. Hum Gene Ther 1999; 10: 1683-94.
187. 187. Hoet PH, Nemery B. Polyamines in the lung: polyamine uptake and polyamine-linked pathological or toxicological conditions. Am J Physiol 2000; 278: L417-33.
188. 188. Morgan DM. Uptake of polyamines by human endothelial cells. Characterization and lack of effect of agonists of endothelial function. Biochem J 1992; 286: 413-7.
189. 189. Hoet PH, Dinsdale D, Lewis CP, Verbeken EK, Lauweryns JM, Nemery B. Kinetics and cellular localisation of putrescine uptake in human lung tissue. Thorax 1993; 48: 1235-41.
190. 190. Sokol PP, Longenecker KL, Kachel DL, Martin WJd. Mechanism of putrescine transport in human pulmonary artery endothelial cells. J Pharmacol Exp Ther 1993; 265: 60-6.
191. 191. Blume G, Cevc G. Liposomes for the sustained drug release in vivo. Biochim Biophys Acta 1990; 1029: 91-7.
192. 192. Kircheis R, Schuller S, Brunner S, et al. Polycation-based DNA complexes for tumor-targeted gene delivery in vivo. J Gene Med 1999; 1: 111-20.
193. 193. Simoes S, Slepushkin V, Gaspar R, de Lima MC, Duzgunes N. Gene delivery by negatively charged ternary complexes of DNA, cationic liposomes and transferrin or fusigenic peptides. Gene Ther 1998; 5: 955-64.
194. 194. Simoes S, Slepushkin V, Pires P, Gaspar R, de Lima MP, Duzgunes N. Mechanisms of gene transfer mediated by lipoplexes associated with targeting ligands or pH-sensitive peptides. Gene Ther 1999; 6: 1798-807.
195. 195. Templeton NS, Lasic DD, Frederik PM, Strey HH, Roberts DD, Pavlakis GN. Improved DNA: liposome complexes for increased systemic delivery and gene expression. Nat Biotechnol 1997; 15: 647-52.
196. 196. Eastman JS, Scheule RK. Cationic lipid:DNA complexes for the treatment of cystic fibrosis. Curr Opin Mol Ther 1999; 1: 186-96.
197. 197. Marshall J, Yew NS, Eastman JS, Jiang C, Scheule RK, Cheng SH, Cationic lipid-mediated gene delivery to the airways. In: Hung M-c, Huang L, Wagner E, eds. Nonviral vectors for gene therapy. San Diego, CA: Academic Press 1999.
198. 198. Sawa Y, Kaneda Y, Bai HZ, et al. Efficient transfer of oligonucleotides and plasmid DNA into the whole heart through the coronary artery. Gene Ther 1998; 5: 1472-80.
199. 199. Zou L, Huang L, Hayes R, et al. Liposome-mediated NGF gene transfection following neuronal injury: Potential therapeutic applications. Gene Ther 1999; 6: 994-1005.
200. 200. Lukacs KV, Nakakes A, Atkins CJ, Lowrie DB, Colston MJ. In vivo gene therapy of malignant tumours with heat shock protein-65 gene. Gene Ther 1997; 4: 346-50.
201. 201. Namiki Y, Takahashi T, Ohno T. Gene transduction for disseminated intraperitoneal tumor using cationic liposomes containing non-histone chromatin proteins: cationic liposomal gene therapy of carcinomatosa. Gene Ther 1998; 5: 240-6.
202. 202. Hottiger MO, Dam TN, Nickoloff BJ, Johnson TM, Nabel GJ. Liposome-mediated gene transfer into human basal cell carcinoma. Gene Ther 1999; 6: 1929-35.
203. 203. Mendiratta SK, Quezada A, Matar M, et al. Intratumoral delivery of IL-12 gene by polyvinyl polymeric vector system to murine renal and colon carcinoma results in potent antitumor immunity. Gene Ther 1999; 6: 833-9.
204. 204. Goldman CK, Soroceanu L, Smith N, et al. In vitro and in vivo gene delivery mediated by a synthetic polycationic amino polymer. Nat Biotechnol 1997; 15: 462-6.
205. 205. Lai LW, Moeckel GW, Lien YH. Kidney-targeted liposome-mediated gene transfer in mice. Gene Ther 1997; 4: 426-31.
206. 206. Baranov A, Glazkov P, Kiselev A, et al. Local and distant transfection of MDX muscle fibers with dystrophin and lacZ genes delivered in vivo by synthetic microspheres. Gene Ther 1999; 6: 1406-14.
207. 207. Coonrod A, Li FQ, Horwitz M. On the mechanism of DNA transfection: efficient gene transfer without viruses. Gene Ther 1997; 4: 1313-21.
208. 208. Zabner J, Fasbender AJ, Moninger T, Poellinger KA, Welsh MJ. Cellular and molecular barriers to genetransfer by a cationic lipid. J Biol Chem 1995; 270: 18997-9007.
209. 209. Tseng WC, Haselton FR, Giorgio TD. Transfection by cationic liposomes using simultaneous single cell measurements of plasmid delivery and transgene expression. J Biol Chem 1997; 272: 25641-7.
210. 210. Capecchi MR, High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell 1980; 22: 479-88.
211. 211. Pires P, Simoes S, Nir S, Gaspar R, Duzgunes N, Pedroso de Lima MC. Interaction of cationic liposomes and their DNA complexes with monocytic leukemia cells. Biochim Biophys Acta 1999; 1418: 71-84.
212. 212. Takeuchi K, Ishihara M, Kawaura C, Noji M, Furuno T, Nakanishi M. Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection. FEBS Lett 1996; 397: 207-9.
213. 213. Pollard H, Remy JS, Loussouarn G, Demolombe S, Behr JP, Escande D. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J Biol Chem 1998; 273: 7507-11.
214. 214. Xu Y, Szoka Jr FC. Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection. Biochemistry 1996; 35: 5616-23.
215. 215. Wrobel I, Collins D. Fusion of cationic liposomes with mammalian cells occurs after endocytosis. Biochim Biophys Acta 1995; 1235: 296-304.
216. 216. El Ouahabi A, Thiry M, Pector V, Fuks R, Ruysschaert JM, Vandenbranden M. The role of endosome destabilizing activity in the gene transfer process mediated by cationic lipids. FEBS Lett 1997; 414: 187-92.
217. 217. Boussif O, Zanta MA, Behr JP. Optimized galenics improve in-vitro gene-transfer with cationic molecules up to 1000-fold. Gene Ther 1996; 3: 1074-80.
218. 218. van de Wetering P, Moret EE, Schuurmans-Nieuwenbroek NM, van Steenbergen MJ, Hennink WE. Structure-activity relationships of water-soluble cationic methacrylate/methacrylamide polymers for nonviral gene delivery. Bioconjug Chem 1999; 10: 589-97.
219. 219. Meunier-Durmort C, Picart R, Ragot T, Perricaudet M, Hainque B, Forest C. Mechanism of adenovirus improvement of cationic liposome-mediated gene transfer. Biochim Biophys Acta 1997; 1330: 8-16.
220. 220. Meunier-Durmort C, Grimal H, Sachs LM, Demeneix BA, Forest C. Adenovirus enhancement of polyethylenimine-mediated transfer of regulated genes in differentiated cells. Gene Ther 1997; 4: 808-14.
221. 221. Dunphy EJ, Redman RA, Herweijer H, Cripe TP. Reciprocal enhancement of gene transfer by combinatorial adenovirus transduction and plasmid DNA transfection in vitro and in vivo. Hum Gene Ther 1999; 10: 2407-17.
222. 222. Kaneda Y, Uchida T, Kim J, Ishiura M, Okada Y. The improved efficient method for introducing macromolecules into cells using HVJ (sendai virus) liposomes with gangliosides. Exp Cell Res 1987; 173: 56-69.
223. 223. Ponimaskin E, Bareesel KK, Markgraf K, et al. Sendai virosomes revisited: reconstitution with exogenous lipids leads to potent vehicles for gene transfer. Virology 2000; 269: 391-403.
224. 224. Wagner E, Plank C, Zatloukal K, Cotten M, Birnstiel ML. Influenza-virus hemagglutinin-HA-2 N-terminal fusogenic peptides augment gene-transfer by transferrin polylysine DNA complexes - toward a synthetic virus-like gene-transfer vehicle. Proc Natl Acad Sci USA 1992; 89: 7934-7938.
225. 225. Schoen P, Chonn A, Cullis PR, Wilschut J, Scherrer P. Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles. Gene Ther 1999; 6: 823-32.
226. 226. Mechtler K, Wagner E. Gene transfer mediated by influenza virus peptides: the role of peptide sequences. New J Chem 1997; 21: 105-11.
227. 227. Lechardeur D, Sohn KJ, Haardt M, et al. Metabolic instability of plasmid DNA in the cytosol: A potential barrier to gene transfer. Gene Ther 1999; 6: 482-97.
228. 228. Ross GF, Bruno MD, Uyeda M, et al. Enhanced reporter gene expression in cells transfected in the presence of dmi-2, an acid nuclease inhibitor. Gene Ther 1998; 5: 1244-50.
229. 229. Zauner W, Brunner S, Buschle M, Ogris M, Wagner E, Differential behaviour of lipid based and polycation based gene transfer systems in transfecting primary human fibroblasts: a potential role of polylysine in nuclear transport. Biochim Biophys Acta 1999; 1428: 57-67.
230. 230. Vitiello L, Chonn A, Wasserman JD, Duff C, Worton RG. Condensation of plasmid DNA with polylysine improves liposome-mediated gene transfer into established and primary muscle cells. Gene Ther 1996; 3: 396-404.
231. 231. Chan CK, Jans DA. Enhancement of polylysine-mediated transferrinfection by nuclear localization sequences: polylysine does not function as a nuclear localization sequence. Hum Gene Ther 1999; 10: 1695-702.
232. 232. Utvik JK, Nja A, Gundersen K. DNA injection into single cells of intact mice. Hum Gene Ther 1999; 10: 291-300.
233. 233. Dowty ME, Williams P, Zhang G, Hagstrom JE, Wolff JA. Plasmid DNA entry into postmitotic nuclei of primary rat myotubes. Proc Natl Acad Sci USA 1995; 92: 4572-6.
234. 234. Tseng W, Purvis NB, Haselton FR, Giorgio TD. Cationic liposomal delivery of plasmid to endothelial cells measured by quantitative flow cytometry. Biotechnol Bioeng 1996; 50: 548-54.
235. 235. Fasbender A, Zabner J, Zeiher BG, Welsh MJ. A low rate of cell proliferation and reduced DNA uptake limit cationic lipid-mediated gene transfer to primary cultures of ciliated human airway epithelia. Gene Ther 1997; 4: 1173-80.
236. 236. Mortimer I, Tam P, MacLachlan I, Graham RW, Saravolac EG, Joshi PB. Cationic lipid-mediated transfection of cells in culture requires mitotic activity. Gene Ther 1999; 6: 403-11.
237. 237. Brunner S, Sauer T, Carotta S, Cotten M, Saltik M, Wagner E. Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus. Gene Ther 2000; 7: 401-7.
238. 238. Darquet AM, Rangara R, Kreiss P, et al. Minicircle: an improved DNA molecule for in vitro and in vivo gene transfer. Gene Ther 1999; 6: 209-18.
239. 239. Kreiss P, Cameron B, Rangara R, et al. Plasmid DNA size does not affect the physicochemical properties of lipoplexes but modulates gene transfer efficiency. Nucleic Acids Res 1999; 27: 3792-8.
240. 240. Pante N, Aebi U. Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 1996; 31: 153-99.
241. 241. Ludtke JJ, Zhang G, Sebestyen MG, Wolff JA. A nuclear localization signal can enhance both the nuclear transport and expression of 1 kb DNA. J Cell Sci 1999; 112: 2033-41.
242. 242. Stewart M. Nuclear pore structure and function. Semin Cell Biol 1992; 3: 267-77.
243. 243. Feldherr CM, Akin D. EM visualization of nucleocytoplasmic transport processes. Electron Microsc Rev 1990; 3: 73-86.
244. 244. Darquet AM, Cameron B, Wils P, Scherman D, Crouzet J. A new DNA vehicle for nonviral gene delivery: super-coiled minicircle. Gene Ther 1997; 4: 1341-9.
245. 245. Leahy P, Carmichael GG, Rossomando EF. Transcription from plasmid expression vectors is increased up to 14-fold when plasmids are transfected as concatemers. Nucleic Acids Res 1997; 25: 449-50.
246. 246. Boulikas T. Putative nuclear-localization signals (NLS) in protein transcription factors. J Cell Biochem 1994; 55: 32-58.
247. 247. Aronsohn AI, Hughes JA. Nuclear localization signal peptides enhance cationic liposome-mediated gene therapy. J Drug Target 1998; 5: 163-9.
248. 248. Branden LJ, Mohamed AJ, Smith CI. A peptide nucleic acid-nuclear localization signal fusion that mediates nuclear transport of DNA. Nat Biotechnol 1999; 17: 784-7.
249. 249. Sebestyen MG, Ludtke JJ, Bassik MC, et al. DNA vector chemistry: The covalent attachment of signal peptides to plasmid DNA. Nat Biotechnol 1998; 16: 80-5.
250. 250. Neves C, Escriou V, Byk G, Scherman D, Wils P. Intracellular fate and nuclear targeting of plasmid DNA. Cell Biol Toxicol 1999; 15: 193-202.
251. 251. Zanta MA, Belguise-Valladier P, Behr JP. Gene delivery: a single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus. Proc Natl Acad Sci USA 1999; 96: 91-6.
252. 252. Godbey WT, Wu KK, Mikos AG. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc Natl Acad Sci USA 1999; 96: 5177-81.
253. 253. Dean DA. Import of plasmid DNA into the nucleus is sequence specific. Exp Cell Res 1997; 230: 293-302.
254. 254. Dean DA, Dean BS, Muller S, Smith LC. Sequence requirements for plasmid nuclear import. Exp Cell Res 1999; 253: 713-22.
255. 255. Vacik J, Dean B, Zimmer W, Dean D. Cell-specific nuclear import of plasmid DNA. Gene Ther 1999; 6: 1006-14.
256. 256. Collas P, Husebye H, Alestrom P. The nuclear localization sequence of the SV40 T antigen promotes transgene uptake and expression in Zebrafish embryo nuclei. Transgenic Res 1996; 5: 451-8.
257. 257. Dachs GU, Patterson AV, Firth JD, et al. Targeting gene expression to hypoxic tumor cells. Nat Med 1997; 3: 515-20.
258. 258. Sarvesvaran J, Going JJ, Milroy R, Kaye SB, Keith WN. Is small cell lung cancer the perfect target for antitelomerase treatment? Carcinogenesis 1999; 20: 1649-51.
259. 259. Wolff JA, Ludtke JJ, Acsadi G, Williams P, Jani A. Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle. Hum Mol Genet 1992; 1: 363-9.
260. 260. Davis HL, Millan CL, Watkins SC. Immune-mediated destruction of transfected muscle fibers after direct gene transfer with antigen-expressing plasmid DNA. Gene Ther 1997; 4: 181-8.
261. 261. Stripecke R, Carmen Villacres M, Skelton D, Satake N, Halene S, Kohn D. Immune response to green fluorescent protein: implications for gene therapy. Gene Ther 1999; 6: 1305-12.
262. 262. Vitiello L, Bockhold K, Joshi PB, Worton RG. Transfection of cultured myoblasts in high serum concentration with DODAC:DOPE liposomes. Gene Ther 1998; 5: 1306-13.
263. 263. Battersby BJ, Grimm R, Huebner S, Cevc G. Evidence for three-dimensional interlayer correlations in cationic lipid-DNA complexes as observed by cryo-electron microscopy. Biochim Biophys Acta 1998; 1372: 379-83.