Preparative scale cell-free expression systems: New tools for the large scale preparation of integral membrane proteins for functional and structural studies

Methods

Volumn 41, Issue 4, 2007, Pages 355-369

  Schwarz, Daniel ^a   Klammt, Christian ^a   Koglin, Alexander ^a   Löhr, Frank ^a   Schneider, Birgit ^a   Dötsch, Volker ^a   Bernhard, Frank ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

barrel proteins; Cell free expression; Detergent; Detergent micelles; G protein coupled receptors; Integral membrane proteins; Labelling of proteins; Liposomes; Multidrug resistance; NMR spectroscopy; Reconstitution; Solubilization; Stable isotopes; Structural analysis; Transporter

Indexed keywords

DETERGENT; DNA; MEMBRANE PROTEIN;

ARTICLE; CELL FREE SYSTEM; DNA TEMPLATE; ESCHERICHIA COLI; LARGE SCALE PRODUCTION; LIQUID; MICELLE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; STRUCTURE ANALYSIS;

BIOCHEMISTRY; CELL-FREE SYSTEM; DETERGENTS; MEMBRANE PROTEINS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN FOLDING; SOLUBILITY;

EID: 33947171595     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2006.07.001     Document Type: Article

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