Functional antibody production using cell-free translation: Effects of protein disulfide isomerase and chaperones

Nature Biotechnology

Volumn 15, Issue 1, 1997, Pages 79-84

  Ryabova, Lyubov A ^a,b,c   Desplancq, Dominique ^a,d   Spirin, Alexander S ^b   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

^c FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH   (Switzerland)

^d CNRS   (France)

Author keywords

Cell free translation system; Disulfide isomerase; Molecular chaperones; Protein folding; Single chain antibody

Indexed keywords

ANTIBODY; CHAPERONE; DISULFIDE; GLUTATHIONE; HEMAGGLUTININ; PROTEIN DISULFIDE ISOMERASE;

ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; DISULFIDE BOND; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; IMMUNOBLOTTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN FOLDING; RADIOIMMUNOASSAY; RNA TRANSLATION;

AMINO ACID SEQUENCE; ANTIBODIES; ANTIBODY FORMATION; BLOTTING, WESTERN; ESCHERICHIA COLI; IMMUNOGLOBULIN FRAGMENTS; ISOMERASES; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; PROTEIN BIOSYNTHESIS; PROTEIN DISULFIDE-ISOMERASE; RECOMBINANT PROTEINS;

EID: 0031021109     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt0197-79     Document Type: Article

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