Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions

Biochemical and Biophysical Research Communications

Volumn 338, Issue 3, 2005, Pages 1346-1352

  Ahn, Jin Ho ^a   Chu, Hun Su ^a   Kim, Tae Wan ^a   Oh, In Seok ^a   Choi, Cha Yong ^a   Hahn, Geun Hee ^b   Park, Chang Gil ^b   Kim, Dong Myung ^b  

^a Seoul National University   (South Korea)

^b Chungnam National University   (South Korea)

Author keywords

Cell free protein synthesis; Endonuclease; Exonuclease; High throughput protein expression; mRNA stability; PCR; rne 131; T7 terminator

Indexed keywords

ENDONUCLEASE; EXONUCLEASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL STRAIN; CELL FREE SYSTEM; DEGRADATION; DNA TEMPLATE; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC STABILITY; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SYNTHESIS;

CELL EXTRACTS; CELL-FREE SYSTEM; ENDORIBONUCLEASES; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; RNA STABILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 27744523608     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.10.094     Document Type: Article

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