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Volumn 16, Issue 2, 1999, Pages 355-358

A modified procedure for fast purification of T7 RNA polymerase

Author keywords

[No Author keywords available]

Indexed keywords

COLUMN CHROMATOGRAPHY; ENZYME PURIFICATION; ESCHERICHIA COLI; RNA POLYMERASE;

EID: 0033166764     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1083     Document Type: Article
Times cited : (56)

References (11)
  • 2
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    • Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro
    • Sampson J. R., Uhlenbeck O. C. Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro. Proc. Natl. Acad. Sci. USA. 85:1988;1033-1037.
    • (1988) Proc. Natl. Acad. Sci. USA , vol.85 , pp. 1033-1037
    • Sampson, J.R.1    Uhlenbeck, O.C.2
  • 5
    • 0022493193 scopus 로고
    • Transcription by T7 RNA polymerase is not zinc-dependent and is abolished on amidomethylation of cysteine-347
    • King G. C., Martin C. T., Pham T. T., Coleman J. E. Transcription by T7 RNA polymerase is not zinc-dependent and is abolished on amidomethylation of cysteine-347. Biochemistry. 25:1986;36-40.
    • (1986) Biochemistry , vol.25 , pp. 36-40
    • King, G.C.1    Martin, C.T.2    Pham, T.T.3    Coleman, J.E.4
  • 6
    • 0023840230 scopus 로고
    • Omp T encodes Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification
    • Grodberg J., Dunn J. J. omp T encodes Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification. J. Bacteriol. 170:1988;1245-1253.
    • (1988) J. Bacteriol. , vol.170 , pp. 1245-1253
    • Grodberg, J.1    Dunn, J.J.2
  • 7
    • 0025748046 scopus 로고
    • Synthesis and purification of large amounts of RNA oligonucleotides
    • Wyatt R. W., Chastain M., Puglisi J. D. Synthesis and purification of large amounts of RNA oligonucleotides. BioTechniques. 11:1991;764-769.
    • (1991) BioTechniques , vol.11 , pp. 764-769
    • Wyatt, R.W.1    Chastain, M.2    Puglisi, J.D.3
  • 8
    • 0030852441 scopus 로고    scopus 로고
    • Constructing and expression of a modular gene encoding bacteriophage T7 RNA polymerase
    • Arnaud N., Cheynet V., Orid G., Mandrand B., Mallet F. Constructing and expression of a modular gene encoding bacteriophage T7 RNA polymerase. Gene. 199:1997;149-156.
    • (1997) Gene , vol.199 , pp. 149-156
    • Arnaud, N.1    Cheynet, V.2    Orid, G.3    Mandrand, B.4    Mallet, F.5
  • 10
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 11
    • 0023651444 scopus 로고
    • Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates
    • Milligan J. F., Groebe D. R., Witherell G. W., Uhlenbeck O. C. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 15:1987;8783-8798.
    • (1987) Nucleic Acids Res. , vol.15 , pp. 8783-8798
    • Milligan, J.F.1    Groebe, D.R.2    Witherell, G.W.3    Uhlenbeck, O.C.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.