A new class of enzyme acting on damaged ribosomes: Ribosomal RNA apurinic site specific lyase found in wheat germ

EMBO Journal

Volumn 18, Issue 22, 1999, Pages 6522-6531

  Ogasawara, Tomio ^a   Sawasaki, Tatsuya ^a   Morishita, Ryo ^a   Ozawa, Akihiko ^a   Madin, Kairat ^a   Endo, Yaeta ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Apurinic site; Cleavage; Lyase; rRNA; Sarcin ricin domain

Indexed keywords

COMPLEMENTARY DNA; DNA; LYASE; RIBOSOME RNA; RICIN; RNA 28S;

AMINO ACID SEQUENCE; APURINIC SITE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DOMAIN; SEQUENCE HOMOLOGY; WHEAT GERM;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, ION EXCHANGE; DNA, COMPLEMENTARY; ENDORIBONUCLEASES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PLANT LECTINS; RECOMBINANT PROTEINS; RIBOSOMES; RICIN; RNA, PLANT; RNA, RIBOSOMAL, 28S; SEEDS; SUBSTRATE SPECIFICITY; TRITICUM;

TRITICUM AESTIVUM;

EID: 0033570907     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/18.22.6522     Document Type: Article

References (34)

1. Barbieri, L., Battelli, M.G. and Stirpe, F. (1993) Ribosome-inactivating proteins from plants. Biochim. Biophys. Acta, 1154, 237-282.
2. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
3. Coligan, J.R., Dunn, B.M., Ploegh, H.L., Speicher, D.W. and Wingfield, P.T. (1995) Current Protocols in Protein Science. John Wiley & Sons, Inc., New York, NY.
4. Correll, C.C., Munishkin, A., Chan, L.-L., Ren, Z., Wool, I.G. and Steitz, T.A. (1998) Crystal structure of the ribosomal RNA domain essential for binding elongation factors. Proc. Natl Acad. Sci. USA, 95, 13436-13441.
5. Endo, Y. and Wool, J.G. (1982) The site of action of α-sarcin on eukaryotic ribosomes. J. Biol. Chem., 257, 9054-9060.
6. Endo, Y. and Tsurugi, K. (1987) RNA N-glycosidase activity of ricin A-chain. J. Biol. Chem., 262, 8128-8130.
7. Endo, Y. and Tsurugi, K. (1988) The RNA N-glycosidase activity of ricin A-chain. J. Biol. Chem., 263, 8735-8739.
8. Endo, Y., Huber, P.W. and Wool, I.G. (1983) The ribonuclease activity of the cytotoxin α-sarcin. J. Biol. Chem., 258, 2662-2667.
9. Endo, Y., Mitsui, K., Motizuki, M. and Tsurugi, K. (1987) The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. J. Biol. Chem., 262, 5908-5912.
10. Endo, Y., Glück, A. and Wool, I.G. (1991) Ribosomal RNA identity elements for ricin A-chain recognition and catalysis. J. Mol. Biol., 221, 193-207.
11. Endo, Y., Otsuzuki, S., Ito, K. and Miura, K. (1992) Production of an enzymatic active protein using a continuous flow cell-free translation system. J. Biotechnol., 25, 221-230.
12. Erickson, A.H. and Blobel, G. (1983) Cell-free translation of messenger RNA in a wheat germ system. Methods Enzymol., 96, 38-50.
13. Fernandez-Puentes, C., Carrasco, L. and Vazquez, D. (1976) Site of action of ricin on the ribosome. Biochemistry, 15, 4364-4369.
14. 2660 of the 23S ribosomal RNA. J. Mol. Biol., 221, 737-743.
15. Hausner, T.-P., Atmadja, J. and Nierhaus, K. (1987) Evidence that the G2661 region of 23S rRNA is located at the ribosomemal binding site of both elongation factors. Biochimie, 69, 911-923.
16. Kawakami, N., Miyake, Y. and Noda, K. (1996) Molecular cloning and expression of a polypeptide that does not accumulate in the embryos of a non-dormant mutant of wheat. In Noda, K. and Mares, D.J. (eds), Pre-Harvest Sprouting in Cereals 1995. Center for Academic Societies Japan, Osaka, Japan, pp. 123-128.
17. Massiah, A.J. and Hartley, M.R. (1995) Wheat ribosome-inactivating proteins: Seed and leaf forms with different specificities and cofactor requirements. Planta, 197, 633-640.
18. 4 catalyzes DNA strand cleavage at aldehydic abasic sites by a syn b-elimination reaction. J. Am. Chem. Soc., 111, 8029-8030.
19. Moazed, D., Robertson, J.M. and Noller, H.F. (1988) Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA. Nature, 334, 362-364.
20. Montanaro, L., Sperti, S., Mattioli, A., Testoni, G. and Stirpe, F. (1975) Inhibition by ricin of protein synthesis in vitro. Biochem. J., 146, 127-131.
21. Nilsson, L. and Nygard, O. (1986) The mechanism of the protein-synthesis elongation cycle in eukaryotes. Eur. J. Biochem., 161, 111-117.
22. Olsnes, S., Fernandez-P, C., Carrasco, L. and Vazquez, D. (1975) Ribosome inactivation by the toxic lectins abrin and ricin. Kinetics of the enzymic activity of the toxin A-chains. Eur. J. Biochem., 60, 281-288.
23. Randerath, E., Yu, C.-T. and Randerath, K. (1972) Base analysis of ribopolynucleotides by chemical tritium labeling: a methodogical study with model nucleosides and purified tRNA species. Anal. Biochem., 48, 172-198.
24. Ready, M.P, Brown, D.T. and Robertus, J.D. (1986) Extracellular localization of pokeweed antiviral protein. Proc. Natl Acad. Sci. USA, 83, 5053-5056.
25. Seeberg, E., Eide, L. and Bjoras, M. (1995) The base excision repair pathway. Trends Biochem. Sci., 20, 391-397.
26. Shaper, N.L. and Grossman, L. (1980) Purification and properties of the human placental apurinic/apyrimidinic endonuclease. Methods Enzymol., 65, 216-224.
27. Stephens, R.S. et al., (1998) Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science, 282, 754-759.
28. Takaiwa, F., Oono, K., Iida, Y. and Sugiura, M. (1985) The complete nucleotide sequence of a rice 25S rRNA gene. Gene, 37, 255-259.
29. Taylor, B.E. and Irvin, J.D. (1990) Depurination of plant ribosomes by pokeweed antiviral protein. FEBS Lett., 273, 144-146.
30. Taylor, S., Massiah, A., Lomonossoff, G., Robert, L.M., Lord, J.M. and Hartley, M.R. (1994) Correlation between the activities of five ribosome-inactivating proteins in depurination of tobacco ribosomes and inhibition of tobacco mosaic virus infection. Plant J., 5, 827-853.
31. Wool, I.G. (1979) The structure and function of eukaryotic ribosomes. Annu. Rev. Biochem., 48, 719-754.
32. Wool, I.G., Glück, A. and Endo, Y. (1992) Ribotoxin recognition of ribosomal RNA and a proposal for the mechanism of translation. Trends Biochem. Sci., 17, 266-269.
33. Yoshinari, S., Yokota, S., Sawamoto, H., Koresawa, S., Tamura, M. and Endo, Y. (1996) Purification, characterization and subcellular localization of a type-1 ribosome-inactivating protein from the sarcocarp of Cucubita pepo. Eur. J. Biochem., 242, 585-591.
34. Yoshinari, S., Koresawa, S., Yokota, S., Sawamoto, H., Tamura, M. and Endo, Y. (1997) Gypsophilin, a new type 1 ribosome-inactivating protein from Gypsophila elegans. Biosci. Biotechnol. Biochem., 61, 324-331.