Prolonging cell-free protein synthesis with a novel ATP regeneration system

Biotechnology and Bioengineering

Volumn 66, Issue 3, 1999, Pages 180-188

  Kim, Dong Myung ^a,b   Swartz, James R ^a,b  

^a GENENTECH INC   (United States)

^b Stanford University   (United States)

Author keywords

Acetyl phosphate; Adenosine triphosphate (ATP) regeneration; Cell free protein synthesis; Chloramphenicol acetyl transferase (CAT); Human lymphotoxin; In vitro protein synthesis; Inorganic phosphate; Phosphoenol pyruvate (PEP); Pyruvate; Pyruvate oxidase

Indexed keywords

ADENOSINETRIPHOSPHATE; BATCH CELL CULTURE; CATALYST ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; PHOSPHATES;

CELL-FREE PROTEIN SYNTHESIS; CHLORAMPHENICOL ACETYL TRANSFERASE; PHOSPHOENOL PYRUVATE;

BIOSYNTHESIS;

ADENOSINE KINASE; CHLORAMPHENICOL ACETYLTRANSFERASE; LYMPHOTOXIN; MAGNESIUM ION; PHOSPHATE; PHOSPHOENOLPYRUVATE; PYRUVATE OXIDASE;

ARTICLE; CATALYSIS; CELL FREE SYSTEM; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PROTEIN SYNTHESIS; PROTEIN SYNTHESIS INHIBITION;

ADENOSINE TRIPHOSPHATE; AMINO ACIDS; BIOTECHNOLOGY; CELL-FREE SYSTEM; CHLORAMPHENICOL O-ACETYLTRANSFERASE; ESCHERICHIA COLI; HUMANS; LYMPHOTOXIN-ALPHA; PHOSPHOENOLPYRUVATE; PHOSPHORIC ACID ESTERS; PYRUVATE OXIDASE; PYRUVIC ACID; RECOMBINANT PROTEINS;

EID: 0033404584     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(1999)66:3<180::AID-BIT6>3.0.CO;2-S     Document Type: Article

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