High level cell-free expression and specific labeling of integral membrane proteins

European Journal of Biochemistry

Volumn 271, Issue 3, 2004, Pages 568-580

  Klammt, Christian ^a   Löhr, Frank ^a   Schäfer, Birgit ^a   Haase, Winfried ^b   Dötsch, Volker ^a   Rüterjans, Heinz ^a   Glaubitz, Clemens ^a   Bernhard, Frank ^a  

^a GOETHE UNIVERSITY   (Germany)

^b MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

Author keywords

Amino acid specific labeling; Cell free expression; Integral membrane proteins; Multidrug transporter; Solution NMR

Indexed keywords

AMINO ACID; AMINO ACID TRANSPORTER; CELL EXTRACT; CYSTEINE; DETERGENT; ETHIDIUM; GLYCOPROTEIN P; HYBRID PROTEIN; LIPID; LIPOSOME; MEMBRANE PROTEIN; NITROGEN 15; PROTEIN EMRE; PROTEIN SUGE; PROTEIN TEHA; PROTEIN YFIK; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; CELL FREE SYSTEM; CIRCULAR DICHROISM; CONTROLLED STUDY; ELECTRON MICROSCOPY; ESCHERICHIA COLI; FLUORESCENCE; FREEZE FRACTURE; GENE CONTROL; GENETIC TRANSCRIPTION; ISOTOPE LABELING; MICELLE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN SYNTHESIS; RNA TRANSLATION; SOLUBILITY; SOLUBILIZATION;

BASE SEQUENCE; CELL-FREE SYSTEM; CIRCULAR DICHROISM; DETERGENTS; DNA PRIMERS; ESCHERICHIA COLI PROTEINS; FREEZE FRACTURING; MEMBRANE PROTEINS; MICROSCOPY, ELECTRON; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; PROTEOLIPIDS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0842265773     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2003.03959.x     Document Type: Article

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