Regeneration of adenosine triphosphate from glycolytic intermediates for cell-free protein synthesis

Biotechnology and Bioengineering

Volumn 74, Issue 4, 2001, Pages 309-316

  Kim, Dong Myung ^a   Swartz, James R ^a  

^a Stanford University   (United States)

Author keywords

Acetylphosphate; Cell free protein synthesis; Chloramphenicol acetyltransferase; Glucose 6 phosphate; Glycolysis; Inorganic phosphate; Phosphoenol pyruvate; Pyruvate

Indexed keywords

COST EFFECTIVENESS; GLUCOSE; PROTEINS; SYNTHESIS (CHEMICAL);

REGENERATION;

ADENOSINETRIPHOSPHATE;

ADENOSINE TRIPHOSPHATE; CHLORAMPHENICOL ACETYLTRANSFERASE; GLUCOSE; GLUCOSE 6 PHOSPHATE; PHOSPHATE; PHOSPHOENOLPYRUVATE; PYRUVIC ACID;

ARTICLE; CELL FREE SYSTEM; ENZYME ACTIVITY; GLYCOLYSIS; INCUBATION TIME; PROTEIN SYNTHESIS; REACTION ANALYSIS;

ADENOSINE TRIPHOSPHATE; AMINO ACIDS; CELL-FREE SYSTEM; ENERGY METABOLISM; GLUCOSE-6-PHOSPHATE; GLYCOLYSIS; KINETICS; PHOSPHOENOLPYRUVATE; PROTEIN BIOSYNTHESIS; PYRUVIC ACID; TIME FACTORS;

EID: 0035921172     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1121     Document Type: Article

References (19)

1. Cell-free synthesis and affinity isolation of proteins on a nanomole scale
2. Preparation of a cell-free protein-synthesizing system from wheat germ
3. A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding
4. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
5. How many EF-Tu molecules participate in aminoacyl-tRNA binding and peptide bond formation in Escherichia coli translation?
6. 32P]ATP in reticulocyte lysate
7. Recent advances in producing and selecting functional proteins by using cell-free translation
8. Cell-free synthesis and acid-selective stable isotope labeling of proteins for NMR analysis
9. A highly efficient cell-free protein synthesis system from E. coli
10. Prolonging cell-free protein synthesis with a novel ATP regeneration system
11. Prolonging cell-free protein synthesis by selective reagent additions
12. Oxalate improves protein synthesis by enhancing ATP supply in a cell-free system derived from Escherichia coli
13. Cleavage of structural proteins during the assembly of the bacteriophage T4
14. Coupled transcription-translation in prokaryotic cell-free systems
15. Continuous gene expression in vitro: The Spirin system
16. Acetyl phosphate as an energy source for bacterial cell-free translation systems
17. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria
18. Identification, localization, and function of the thiamine pyrophosphate and flavin adenine dinucleotide dependent pyruvate oxidase in isolated membrane vesicles of Escherichia coli B
19. The potentials of the in vitro protein biosynthesis system