Genetic and cellular toxicology of dental resin monomers

Journal of Dental Research

Volumn 85, Issue 10, 2006, Pages 870-877

  Schweikl, H ^a   Spagnuolo, G ^b   Schmalz, G ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Apoptosis; Cell cycle; Dental materials; Genotoxicity; Oxidative stress

Indexed keywords

EID: 33750045758     PISSN: 00220345     EISSN: None     Source Type: Journal    
DOI: 10.1177/154405910608501001     Document Type: Review

References (124)

1. Achanta G, Huang P (2004). Role of p53 in sensing oxidative DNA damage in response to reactive oxygen species-generating agents. Cancer Res 64:6233-6239.
2. Adimoolam S, Ford JM (2003). p53 and regulation of DNA damage recognition during nucleotide excision repair. DNA Repair (Amst) 2:947-954.
3. Alanko K, Susitaival P, Jolanki R, Kanerva L (2004). Occupational skin diseases among dental nurses. Contact Dermatitis 50:77-82.
4. Ames BN (1983). Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science 221:1256-1264.
5. Ashby J (2002). Scientific issues associated with the validation of in vitro and in vivo methods for assessing endocrine disrupting chemicals. Toxicology 181-182:389-397.
6. Bakkenist CJ, Kastan MB (2004). Initiating cellular stress responses. Cell 118:9-17.
7. Barzilai A, Yamamoto K (2004). DNA damage responses to oxidative stress. DNA Repair (Amst) 3:1109-1115.
8. Beinke S, Ley SC (2004). Functions of NF-kappaB1 and NF-kappaB2 in immune cell biology. Biochem J 382(Pt 2):393-409.
9. Besaratinia A, Pfeifer GP (2005). DNA adduction and mutagenic properties of acrylamide. Mutat Res 580:31-40.
10. Boonstra J, Post JA (2004). Molecular events associated with reactive oxygen species and cell cycle progression in mammalian cells. Gene 337:1-13.
11. Borisenko GG, Martin I, Zhao Q, Amoscato AA, Tyurina YY, Kagan VE (2004). Glutathione propagates oxidative stress triggered by myeloperoxidase in HL-60 cells. Evidence for glutathionyl radical-induced peroxidation of phospholipids and cytotoxicity. J Biol Chem 279:23453-23462.
12. Bouillaguet S (2004). Biological risks of resin-based materials to the dentin-pulp complex. Crit Rev Oral Biol Med 15:47-60.
13. Bouillaguet S, Wataha JC, Hanks CT, Ciucchi B, Holz J (1996). In vitro cytotoxicity and dentin permeability of HEMA. J Endod 22:244-248.
14. Cerutti PA (1985). Prooxidant states and tumor promotion. Science 227:375-381.
15. Chang HH, Guo MK, Kasten FH, Chang MC, Huang GF, Wang YL, et al. (2005). Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 26:745-753.
16. Cimpan MR, Matre R, Cressey LI, Tysnes B, Lie SA, Gjertsen BT, et al. (2000a). The effect of heat- and auto-polymerized denture base polymers on clonogenicity, apoptosis, and necrosis in fibroblasts: denture base polymers induce apoptosis and necrosis. Acta Odontol Scand 58:217-228.
17. Cimpan MR, Cressey LI, Skaug N, Halstensen A, Lie SA, Gjertsen BT, et al. (2000b). Patterns of cell death induced by eluates from denture base acrylic resins in U-937 human monoblastoid cells. Eur J Oral Sci 108:59-69.
18. Daniel PT, Schulze-Osthoff K, Belka C, Guner D (2003). Guardians of cell death: the Bcl-2 family proteins. Essays Biochem 39:73-88.
19. De Flora S, Izzotti A, D'Agostini F, Balansky RM (2001). Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis 22:999-1013.
20. Dent P, Yacoub A, Fisher PB, Hagan MP, Grant S (2003). MAPK pathways in radiation responses. Oncogene 22:5885-5896.
21. Djavaheri-Mergny M, Javelaud D, Wietzerbin J, Besancon F (2004). NF-kappaB activation prevents apoptotic oxidative stress via an increase of both thioredoxin and MnSOD levels in TNFalpha-treated Ewing sarcoma cells. FEBS Lett 578:111-115.
22. Donovan M, Cotter TG (2004). Control of mitochondrial integrity by Bcl-2 family members and caspase-independent cell death. Biochim Biophys Acta 1644:133-147.
23. Downward J (2004). PI 3-kinase, Akt and cell survival. Semin Cell Dev Biol 15:177-182.
24. Droge W (2002). Free radicals in the physiological control of cell function. Physiol Rev 82:47-95.
25. Engelberg D (2004). Stress-activated protein kinases-tumor suppressors or tumor initiators? Semin Cancer Biol 14:271-282.
26. Engelmann J, Leyhausen G, Leibfritz D, Geurtsen W (2002). Effect of TEGDMA on the intracellular glutathione concentration of human gingival fibroblasts. J Biomed Mater Res 63:746-751.
27. Engelmann J, Janke V, Volk J, Leyhausen G, von Neuhoff N, Schlegelberger B, et al. (2004). Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro. Biomaterials 25:4573-4580.
28. Feinendegen LE (2002). Reactive oxygen species in cell responses to toxic agents. Hum Exp Toxicol 21:85-90.
29. Geurtsen W (2000). Biocompatibility of resin-modified filling materials. Crit Rev Oral Biol Med 11:333-355.
30. Geurtsen W (2003). Toxicology of dental materials and 'clinical experience' [editorial]. J Dent Res 82:500.
31. Geurtsen W, Leyhausen G (2001). Chemical-biological interactions of the resin monomer triethyleneglycol-dimethacrylate (TEGDMA). J Dent Res 80:2046-2050.
32. Goldberg M, Smith AJ (2004). Cells and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering. Crit Rev Oral Biol Med 15:13-27.
33. Gough JE, Downes S (2001). Osteoblast cell death on methacrylate polymers involves apoptosis. J Biomed Mater Res 57:497-505.
34. Haddad JJ (2002). Antioxidant and prooxidant mechanisms in the regulation of redox(y)-sensitive transcription factors. Cell Signal 14:879-897.
35. Haddad JJ (2004). Oxygen sensing and oxidant/redox-related pathways. Biochem Biophys Res Commun 316:969-977.
36. Hanks CT, Strawn SE, Wataha JC, Craig RG (1991). Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 70:1450-1455.
37. Hayes JD, Flanagan JU, Jowsey IR (2005). Glutathione transferases. Annu Rev Pharmacol Toxicol 45:51-88.
38. Heil J, Reifferecheid G, Waldmann P, Leyhausen G, Geurtsen W (1996). Genotoxicity of dental materials. Mutat Res 368:181-194.
39. Hume WR, Gerzina TM (1996). Bioavailability of components of resin-based materials which are applied to teeth. Crit Rev Oral Biol Med 7:172-179.
40. Izumi T, Wiederhold LR, Roy G, Roy R, Jaiswal A, Bhakat KK, et al. (2003). Mammalian DNA base excision repair proteins: their interactions and role in repair of oxidative DNA damage. Toxicology 193:43-65.
41. Janke V, von Neuhoff N, Schlegelberger B, Leyhausen G, Geurtsen W (2003). TEGDMA causes apoptosis in primary human gingival fibroblasts. J Dent Res 82:814-818.
42. Kaina B (2003). DNA damage-triggered apoptosis: critical role of DNA repair, double-strand breaks, cell proliferation and signaling. Biochem Pharmacol 66:1547-1554.
43. Kanerva L (2001). Cross-reactions of multifunctional methacrylates and acrylates. Acta Odontol Scand 59:320-329.
44. Kanerva L, Estlander T, Jolanki R (1994). Occupational skin allergy in the dental profession. Dermatol Clin 12:517-532.
45. Karin M, Lin A (2002). NF-kappaB at the crossroads of life and death. Nat Immunol 3:221-227.
46. Karin M, Cao Y, Greten FR, Li ZW (2002). NF-kappaB in cancer: from innocent bystander to major culprit. Nat Rev Cancer 2:301-310.
47. Katoh Y, Iida K, Kang MI, Kobayashi A, Mizukami M, Tong KI, et al. (2005). Evolutionary conserved N-terminal domain of Nrf2 is essential for the Keap1-mediated degradation of the protein by proteasome. Arch Biochem Biophys 433:342-350.
48. Kaufmann T, Schinzel A, Bomer C (2004). Bcl-w(edding) with mitochondria. Trends Cell Biol 14:8-12.
49. Kleinsasser NH, Wallner BC, Harreus UA, Kleinjung T, Folwaczny M, Hickel R, et al. (2004). Genotoxicity and cytotoxicity of dental materials in human lymphocytes as assessed by the single cell microgel electrophoresis (comet) assay. J Dent 32:229-234.
50. Kleinsasser NH, Schmid K, Sassen AW, Harreus UA, Staudenmaier R, Folwaczny M, et al. (2006). Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay. Biomaterials 27:1762-1770.
51. Kyosseva SV (2004). Mitogen-activated protein kinase signaling. Int Rev Neurobiol 59:201-220.
52. Lavin MF, Birrell G, Chen P, Kozlov S, Scott S, Gueven N (2005). ATM signaling and genomic stability in response to DNA damage. Mutat Res 569:123-132.
53. Leevers SJ, Vanhaesebroeck B, Waterfield MD (1999). Signalling through phosphoinositide 3-kinases: the lipids take centre stage. Curr Opin Cell Biol 11:219-225.
54. Lefeuvre M, Bourd K, Loriot MA, Goldberg M, Beaune P, Perianin P, et al. (2004). TEGDMA modulates glutathione transferase P1 activity in gingival fibroblasts. J Dent Res 83:914-919.
55. Lefeuvre M, Amjaad W, Goldberg M, Stanislawski L (2005). TEGDMA induces mitochondrial damage and oxidative stress in human gingival fibroblasts. Biomaterials 26:5130-5137.
56. Levonen AL, Landar A, Ramachandran A, Ceaser EK, Dickinson DA, Zanoni G, et al. (2004). Cellular mechanisms of redox cell signalling: role of cysteine modification in controlling antioxidant defences in response to electrophilic lipid oxidation products. Biochem J 378(Pt 2):373-382.
57. Luchsinger JA, Mayeux R (2004). Dietary factors and Alzheimer's disease. Lancet Neurol 3:579-587.
58. Majno G, Joris I (1995). Apoptosis, oncosis, and necrosis. An overview of cell death. Am J Pathol 146:3-15.
59. Mantellini MG, Botero TM, Yaman P, Dennison JB, Hanks CT, Nör JE (2003). Adhesive resin induces apoptosis and cell-cycle arrest of pulp cells. J Dent Res 82:592-596.
60. Marnett LJ (1994). DNA adducts of alpha, beta-unsaturated aldehydes and dicarbonyl compounds. IARC Sci Publ 125:151-163.
61. Marshall HE, Merchant K, Stamler JS (2000). Nitrosation and oxidation in the regulation of gene expression. FASEB J 14:1889-1900.
62. Mathers J, Fraser JA, McMahon M, Saunders RD, Hayes JD, McLellan LI (2004). Antioxidant and cytoprotective responses to redox stress. Biochem Soc Symp 71:157-176.
63. Mattson MP (2004). Pathways towards and away from Alzheimer's disease. Nature 430:631-639
64. Michelsen VB, Lygre H, Skalevik R, Tveit AB, Solheim E (2003). Identification of organic eluates from four polymer-based dental filling materials. Eur J Oral Sci 111:263-271.
65. Michiels C, Minet E, Mottet D, Raes M (2002). Regulation of gene expression by oxygen: NF-kappaB and HIF-1, two extremes. Free Radic Biol Med 33:1231-1242.
66. Mjör IA, Ferrari M (2002). Pulp-dentin biology in restorative dentistry. Part 6: Reactions to restorative materials, tooth-restoration interfaces, and adhesive techniques. Quintessence Int 33:35-63.
67. Niki E, Noguchi N (2004). Dynamics of antioxidant action of vitamin E. Acc Chem Res 37:45-51.
68. Nioi P, Hayes JD (2004). Contribution of NAD(P)H:quinone oxidoreductase 1 to protection against carcinogenesis, and regulation of its gene by the Nrf2 basic-region leucine zipper and the arylhydrocarbon receptor basic helix-loop-helix transcription factors. Mutat Res 555:149-171.
69. Noda M, Wataha JC, Kaga M, Lockwood PE, Volkmann KR, Sano H (2002). Components of dentinal adhesives modulate heat shock protein 72 expression in heat-stressed THP-1 human monocytes at sublethal concentrations. J Dent Res 81:265-269.
70. Noda M, Wataha JC, Lockwood PE, Volkmann KR, Kaga M, Sano H (2003). Sublethal, 2-week exposures of dental material components alter TNF-alpha secretion of THP-1 monocytes. Dent Mater 19:101-105.
71. Noda M, Wataha JC, Lewis JB, Kaga M, Lockwood PE, Messer RL, et al. (2005). Dental adhesive compounds alter glutathione levels but not glutathione redox balance in human THP-1 monocytic cells. J Biomed Mater Res B Appl Biomater 73:308-314.
72. Nyberg KA, Michelson RJ, Putnam CW, Weinert TA (2002). Toward maintaining the genome: DNA damage and replication checkpoints. Annu Rev Genet 36:617-656.
73. Ortengren U (2000). On composite resin materials. Degradation, erosion and possible adverse effects in dentists. Swed Dent J Suppl 141:1-61.
74. Pagoria D, Lee A, Geurtsen W (2005). The effect of camphorquinone (CQ) and CQ-related photosensitizers on the generation of reactive oxygen species and the production of oxidative DNA damage. Biomaterials 26:4091-4099.
75. Paranjpe A, Bordador LC, Wang MY, Hume WR, Jewett A (2005). Resin monomer 2-hydroxyethyl methacrylate (HEMA) is a potent inducer of apoptotic cell death in human and mouse cells. J Dent Res 84:172-177.
76. Peutzfeldt A (1997). Resin composites in dentistry: the monomer systems. Eur J Oral Sci 105:97-116.
77. Pfeiffer P, Feldmann E, Odersky A, Kuhfittig-Kulle S, Goedecke W (2005). Analysis of DNA double-strand break repair by nonhomologous end joining in cell-free extracts from mammalian cells. Methods Mol Biol 291:351-371.
78. Pham CG, Bubici C, Zazzeroni F, Papa S, Jones J, Alvarez K, et al. (2004). Ferritin heavy chain upregulation by NF-kappaB inhibits TNFalpha-induced apoptosis by suppressing reactive oxygen species. Cell l19:529-542.
79. Pulgar R, Olea-Serrano MF, Novillo-Fertrell A, Rivas A, Pazos P, Pedraza V, et al. (2000). Determination of bisphenol A and related aromatic compounds released from bis-GMA-based composites and sealants by high performance liquid chromatography. Environ Health Perspect 108:21-27.
80. Qin C, Baba O, Butler WT (2004). Post-translational modifications of sibling proteins and their roles in osteogenesis and dentinogenesis. Crit Rev Oral Biol Med 15:126-136.
81. Quinlan CA, Zisterer DM, Tipton KF, O'Sullivan MI (2002). In vitro cytotoxicity of a composite resin and compomer. Int Endod J 35:47-55.
82. Rahman I, MacNee W (2000). Regulation of redox glutathione levels and gene transcription in lung inflammation: therapeutic approaches. Free Radic Biol Med 28:1405-1420.
83. Rueggeberg FA (2002). From vulcanite to vinyl, a history of resins in restorative dentistry. J Prosthet Dent 87:364-379.
84. Saelens X, Festjens N, Vande Walle L, van Gurp M, van Loo G, Vandenabeele P (2004). Toxic proteins released from mitochondria in cell death. Oncogene 23:2861-2874.
85. Sagrista ML, Garcia AE, Africa De Madariaga M, Mora M (2002). Antioxidant and pro-oxidant effect of the thiolic compounds N-acetyl-L-cysteine and glutathione against free radical-induced lipid peroxidation. Free Radic Res 36:329-340.
86. Sakon S, Xue X, Takekawa M, Sasazuki T, Okazaki T, Kojima Y, et al. (2003) NF-kappaB inhibits TNF-induced accumulation of ROS that mediate prolonged MAPK activation and necrotic cell death. EMBO J 22:3898-3909.
87. Samuelsen JT, Dahl JE, Karlsson S, Morisbak E, Becher R (2006). Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK. Dent Mater, available online 20 January [E-pub ahead of print].
88. Sancar A, Lindsey-Boltz LA, Unsal-Kacmaz K, Linn S (2004). Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints. Annu Rev Biochem 73:39-85.
89. Santerre JP, Shajii L, Leung BW (2001). Relation of dental composite formulations to their degradation and the release of hydrolyzed polymeric-resin-derived products. Crit Rev Oral Biol Med 12:136-151.
90. Schmalz G (1998). The biocompatibility of non-amalgam dental filling materials. Eur J Oral Sci 106(2 Pt 2):696-706.
91. Schmalz G (2002). Materials science: biological aspects. J Dent Res 81:660-663.
92. Schmitz ML, Mattioli I, Buss H, Kracht M (2004). NF-kappaB: a multifaceted transcription factor regulated at several levels. ChemBioChem 5:1348-1358.
93. Schuster GS, Caughman GB, Rueggeberg FA (2000). Changes in cell phospholipid metabolism in vitro in the presence of HEMA and its degradation products. Dent Mater 16:297-302.
94. Schweikl H, Schmalz G (1999). Triethylene glycol dimethacrylate induces large deletions in the hprt gene of V79 cells. Mutat Res 438:71-78.
95. Schweikl H, Schmalz G, Rackebrandt K (1998). The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells. Mutat Res 415:119-130.
96. Schweikl H, Schmalz G, Spruss T (2001). The induction of micronuclei in vitro by unpolymerized resin monomers. J Dent Res 80:1615-1620.
97. Schweikl H, Hiller KA, Bolay C, Kreissl M, Kreismann W, Nusser A, et al. (2005a). Cytotoxic and mutagenic effects of dental composite materials. Biomaterials 26:1713-1719.
98. Schweikl H, Altmannberger I, Hanser N, Hiller KA, Bolay C, Brockhoff G, et al. (2005b). The effect of triethylene glycol dimethacrylate on the cell cycle of mammalian cells. Biomaterials 26:4111-4118.
99. Schweikl H, Hartmann A, Hiller KA, Spagnuolo G, Bolay C, Brockhoff G, et al (2006). Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. Dent Mater, available online 7 August 2006.
100. Shackelford RE, Kaufmann WK, Paules RS (1999). Cell cycle control, checkpoint mechanisms, and genotoxic stress. Environ Health Perspect 107(Suppl 1):5-24.
101. Shackelford RE, Kaufmann WK, Paules RS (2000). Oxidative stress and cell cycle checkpoint function. Free Radic Biol Med 28:1387-1404.
102. Solomon JJ (1994). DNA adducts of lactones, sultones, acylating agents and acrylic compounds. IARC Sci Publ 125:179-198.
103. Spagnuolo G, Galler K, Schmalz G, Cosentino C, Rengo S, Schweikl H (2004a). Inhibition of phosphatidylinositol 3-kinase amplifies TEGDMA-induced apoptosis in primary human pulp cells. J Dent Res 83:703-707.
104. Spagnuolo G, Mauro C, Leonardi A, Santillo M, Paterno R, Schweikl H, et al. (2004b). NF-kappaB protection against apoptosis induced by HEMA. J Dent Res 83:837-842.
105. Spagnuolo G, D'Antò V, Cosentino C, Schmalz G, Schweikl H, Rengo S (2006). Effect of N-acetyl-L-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts. Biomaterials 27:1803-1809.
106. Stanislawski L, Lefeuvre M, Bourd K, Soheili-Majd E, Goldberg M, Perianin A (2003). TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. J Biomed Mater Res A 66:476-482.
107. Tanoue T, Nishida E (2003). Molecular recognitions in the MAP kinase cascades. Cell Signal 15:455-462.
108. Thorbum A (2004). Death receptor-induced cell killing. Cell Signal 16:139-144.
109. Torres M, Forman HJ (2003). Redox signaling and the MAP kinase pathways. Biofactors 17:287-296.
110. Volk J, Engelmann J, Leyhausen G, Geurtsen W (2006). Effects of three resin monomers on the cellular glutathione concentration of cultured human gingival fibroblasts. Dent Mater 22:499-505.
111. Volkel W, Colnot T, Csanady GA, Filser JG, Dekant W (2002). Metabolism and kinetics of bisphenol A in humans at low doses following oral administration. Chem Res Toxicol 15:1281-1287.
112. Wada H, Tarumi H, Imazato S, Narimatsu M, Ebisu S (2004). In vitro estrogenicity of resin composites. J Dent Res 83:222-226.
113. Wataha JC, Rueggeberg FA, Lapp CA, Lewis JB, Lockwood PE, Ergle JW, et al. (1999). In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva. Clin Oral Investig 3:144-149.
114. Walther UI, Siagian II, Walther SC, Reichl FX, Hickel R (2004). Antioxidative vitamins decrease cytotoxicity of HEMA and TEGDMA in cultured cell lines. Arch Oral Biol 49:125-131.
115. Watson WH, Yang X, Choi YE, Jones DP, Kehrer JP (2004). Thioredoxin and its role in toxicology. Toxicol Sci 78:3-14.
116. Wenger RH (2000). Mammalian oxygen sensing, signalling and gene regulation. J Exp Biol 203(Pt 8):1253-1263.
117. West KA, Castillo SS, Dennis PA (2002). Activation of the PI3K/Akt pathway and chemotherapeutic resistance. Drug Resist Updat 5:234-248.
118. Winter K, Pagoria D, Geurtsen W (2005). The effect of antioxidants on oxidative DNA damage induced by visible-light-irradiated camphorquinone/N,N- dimemyl-p-toluidine. Biomaterials 26:5321-5329.
119. Wu G, Fang YZ, Yang S, Lupton JR, Turner ND (2004). Glutathione metabolism and its implications for health. J Nutr 134:489-492.
120. Yoshii E (1997). Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J Biomed Mater Res 37:517-524.
121. Yu C, Rahmani M, Almenara J, Sausville EA, Dent P, Grant S (2004). Induction of apoptosis in human leukemia cells by the tyrosine kinase inhibitor adaphostin proceeds through a RAF-1/MEK/ERK- and AKT-dependent process. Oncogene 23:1364-1376.
122. Zafarullah M, Li WQ, Sylvester J, Ahmad M (2003). Molecular mechanisms of N-acetylcysteine actions. Cell Mol Life Sci 60:6-20.
123. Zecca L, Youdim MB, Riederer P, Connor JR, Crichton RR (2004). Iron, brain ageing and neurodegenerative disorders. Nat Rev Neurosci 5:863-873.
124. Zhivotovsky B (2004). Apoptosis, necrosis and between. Cell Cycle 3:64-66.