Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay

Biomaterials

Volumn 27, Issue 9, 2006, Pages 1762-1770

  Kleinsasser, Norbert H ^a   Schmid, Katharina ^a   Sassen, Andrea W ^a   Harréus, Ulrich A ^b   Staudenmaier, Rainer ^a   Folwaczny, Matthias ^b   Glas, Jürgen ^b   Reichl, Franz Xaver ^b  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Comet assay; Human cells; Lymphocytes; Resin monomers; Salivary glands; Tumorigenesis

Indexed keywords

BIOASSAY; CELLS; DIFFUSION; DNA; HUMAN ENGINEERING; RESINS; TISSUE; TUMORS;

COMET ASSAY; CYTOTOXICITY EFFECTS; GENOTOXIC EFFECTS; RESIN MONOMERS; TISSUE CELLS;

MONOMERS;

2 HYDROXYETHYL METHACRYLATE; DIMETHYL SULFOXIDE; DNA; METHACRYLIC ACID DERIVATIVE; METHYLNITRONITROSOGUANIDINE; MONOMER; RESIN; TRIETHYLENE GLYCOL DIMETHACRYLATE; TRYPAN BLUE; UNCLASSIFIED DRUG; URETHANEDIMETHACRYLATE; XENOBIOTIC AGENT;

ADULT; AGED; ALKALINITY; ARTICLE; CELL VIABILITY; COMET ASSAY; CONTROLLED STUDY; CYTOTOXICITY; DNA STRAND BREAKAGE; DOSE RESPONSE; DRUG EFFECT; DRUG MONITORING; EXCISION REPAIR; FEMALE; GENOTOXICITY; HEALTH STATUS; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; MALE; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SALIVARY GLAND; SAMPLING; STAINING; VOLUNTEER;

AGED; AGED, 80 AND OVER; COMET ASSAY; COMPOSITE RESINS; DENTAL MATERIALS; DNA; DNA DAMAGE; FEMALE; HUMANS; LYMPHOCYTES; MALE; METHACRYLATES; MIDDLE AGED; POLYETHYLENE GLYCOLS; POLYMETHACRYLIC ACIDS; POLYURETHANES; SALIVARY GLANDS;

EID: 28744447404     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2005.09.023     Document Type: Article

References (45)

1. N.H. Kleinsasser, B.C. Wallner, U.A. Harréus, T. Kleinjung, M. Folwaczny, and R. Hickel Genotoxicity and cytotoxicity of dental materials in human lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay J Dent 32 2004 229 234
2. P.L. Horn-Ross, B.M. Ljung, and M. Morrow Environmental factors and the risk of salivary gland cancer Epidemiology 8 1997 414 419
3. U.A. Harréus, P. Baumeister, B.C. Wallner, A. Berghaus, and N.H. Kleinsasser Karzinogene und kokarzinogene Effekte von Metallen und Ethylalkohol in humanen Speicheldrüsenzellen HNO 53 2005 155 162
4. W. Geurtsen Substances released from dental resin composites and glass ionomer cements Eur J Oral Sci 106 1998 687 695
5. W. Spahl, H. Budzikiewicz, and W. Geurtsen Determination of leachable components from four commercial dental composites by gas and liquid chromatography/mass spectrometry J Dent 26 1998 137 145
6. U. Ortengren, H. Wellendorf, S. Karlsson, and I.E. Ruyter Water sorption and solubility of dental composites and identification of monomers released in an aqueous environment Oral Rehab 12 2001 1106 1115
7. A. Hensten-Pettersen Skin and mucosal reactions associated with dental materials Eur J Oral Sci 106 1998 707 712
8. G. Schmalz The biocompatibility of non-amalgam dental filling materials Eur J Oral Sci 106 1998 696 706
9. W. Geurtsen Biocompatibility of resin-modified filling materials Crit Rev Oral Biol Med 11 2000 333 355
10. J. Tanaka, T. Hashimoto, J.W. Stansbury, J.M. Antonucci, and K. Suzuki Polymer properties on resins composed of UDMA and methacrylates with the carboxyl group Dent Mater J 20 2001 206 215
11. T.M. Gerzina, and W.R. Hume Effect of dentine on release of TEGDMA from resin composite in vitro J Oral Rehab 21 1994 463 468
12. T.M. Gerzina, K. Picker, A. Hood, and W. Hume Toxicity and quantitative analysis of TEGDMA and composite resin eluates J Dent Res 70 S 1991 424
13. F.X. Reichl, J. Durner, R. Hickel, K.H. Kunzelmann, A. Jewett, and M.Y. Wang Distribution and excretion of TEGDMA in guinea pigs and mice J Dent Res 80 2001 1412 1415
14. Maier H, Sennewald E. Plattenepithelkarzinome. Risikofaktoren für Plattenepithelkarzinome im Kopf-Hals-Bereich: Ergebnisse der Heidelberger Fallkontrollstudien. Hauptverband der gewerblichen Berufsgenossenschaften, Sankt Augustin, 1994.
15. IARC Wood dust IARC Monogr Eval Carcinog Risks Hum 62 1995 35 215
16. J. Singh, D.L. Carlisle, D.E. Pritchard, and S.R. Patierno Chromium-induced genotoxicity and apoptosis: relationship to chromium carcinogenesis [review] Oncol Rep 5 1998 1307 1318
17. O. Kleinsasser, and H.G. Schroeder Adenocarcinomas of the inner nose after exposure to wood dust. Morphological findings and relationships between histopathology and clinical behavior in 79 cases Arch Otorhinolaryngol 245 1988 1 15
18. Jacob P. Wirkung von Pestiziden und Metallen an menschlichem Parotisgewebe - eine experimentelle Studie von Pentachlorophenol, Lindan, Cadmiumsulfat und Natriumdichromat. Dissertation zur Erlangung des Doktorgrades der Medizin der Medizinischen Fakultät der Universität Ulm, 2001.
19. N.H. Kleinsasser, E.R. Kastenbauer, H. Weissacher, R.K. Muenzenrieder, and U.A. Harréus Phthalates demonstrate genotoxicity on human mucosa of the upper aerodigestive tract Environ Mol Mutagen 35 2000 9 12
20. A.R. Collins The comet assay for DNA damage and repair: principles, applications and limitations Mol Biotechnol 53 2005 155 162
21. V.J. McKelvey-Martin, M.H. Green, P. Schmezer, B.L. Pool-Zobel, M.P. De Meo, and A. Collins The single cell electrophoreses assay: a European review Mutat Res 288 1993 47 63
22. E. Rojas, M.C. Lopez, and M. Valverde Single cell electrophoreses assay: methodology and applications J Chromatogr B Biomed Sci Appl 722 1999 225 254
23. S. Cotelle, and J.F. Ferard Comet assay in genetic ecogenotoxicology: a review Environ Mol Mutagen 34 1999 246 255
24. R.R. Tice, E. Agurell, D. Anderson, B. Burlinson, A. Hartmann, and H. Kobayashi Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing Environ Mol Mutagen 35 2000 206 221
25. N.H. Kleinsasser, B.C. Wallner, E.R. Kastenbauer, R.K. Muenzenrieder, and U.A. Harréus Comparing genotoxic sensitivities of peripheral lymphocytes and mucosa of the upper aerodigestive tract of human donors using the Comet assay Mutat Res 467 2000 21 30
26. H.J. Phillips Dye exclusion test for cell viability P.F. Kruse M.K. Patterson Tissue culture methods and applications 1973 Academic Press New York 406 408
27. N. Rajaee-Behbahani, U. Müller-Vogt, R. Gliniorz, E. Engelhardt, H. Bartsch, and P. Schmezer Effect of long-term cryopreservation on mutagen sensitivity and DNA repair capacity of peripheral human lymphocytes J Cancer Res Clin Oncol 125 S 1999 32
28. P.L. Olive, R.E. Durand, J. Le Riche, I.A. Olivotto, and S.M. Jackson Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancer Cancer Res 53 1993 733 736
29. Y. Finer, F. Jaffer, and J.P. Santerre Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites Biomaterials 25 2004 1787 1793
30. B.A. Lin, F. Jaffer, M.D. Duff, Y.W. Tang, and J.P. Santerre Identifying enzyme activities within human saliva which are relevant to dental resin composite biodegradation Biomaterials 26 2005 4259 4264
31. U. Kedjarune, N. Charoenworaluk, and S. Koontongkaew Release of MMA from heat-cured and autopolymerized resins: cytotoxicity testing related to residual monomer Aust Dent J 44 1999 25 30
32. W.R. Hume, and T.M. Gerzina Bioavailabilty of components of resin-based materials which are applied to teeth Crit Oral Biol Med 7 1996 172 179
33. S. Baker, S.C. Brooks, and D.M. Walker The release of residual monomeric methyl methacrylate from acrylic appliances in the human mouth: an assay for monomer in saliva J Dent Res 67 1988 1295 1299
34. P. Khalichi, D.G. Cvitkovitch, and J.P. Santerre Effect of composite resin biodegradation products on oral streptococcal growth Biomaterials 25 2004 5467 5472
35. I. Sideridou, D.S. Achilias, and E. Kyrikou Thermal expansion characteristics of light-cured dental resins and resin composites Biomaterials 25 2004 3087 3097
36. L.A. Hussain, S.H. Dickens, and R.L. Bowen Shear bond strength of experimental methacrylated beta-cyclodextrin-based formulations Biomaterials 26 2005 3973 3979
37. U.I. Walther, I.I. Siagian, S.C. Walther, F.X. Reichl, and R. Hickel Antioxidative vitamins decrease cytotoxicity of HEMA and TEGDMA in cultured cell lines Arch Oral Biol 49 2004 125 131
38. T.H. Huang, H. Lee, and C.T. Kao Evaluation of the genotoxicity of zinc oxide eugenol-based, calcium hydroxide-based, and epoxy resin-based root canal sealers by comet assay J Endod 12 2001 744 748
39. H. Schweikl, I. Altmannsberger, N. Hanser, K.A. Hiller, C. Bolay, and G. Brockhoff The effect of triethylene glykol dimethacrylate on the cell cycle of mammalian cells Biomaterials 26 2005 4111 4118
40. L. Henderson, A. Wolfreys, J. Fedyk, C. Bourner, and S. Windebank The ability of the Comet assay to discriminate between genotoxins and cytotoxins Mutagenesis 13 1998 89 95
41. P.L. Olive, and J.P. Banath Multicell spheroid response to drugs predicted with the comet assay Cancer Res 57 1997 5528 5533
42. H. Schweikl, and G. Schmalz Glutaraldehyde-containing dentin bonding agents are mutagens in mammalian cells in vitro J Biomed Mater Res 36 1997 284 288
43. H. Schweikl, G. Schmalz, and T. Spruss The induction of micronuclei in vitro by unpolymerized resin monomers J Dent Res 80 2001 1615 1620
44. H. Schweikl, and G. Schmalz Triethylene glyco dimethacrylate induces large deletions in the hprt gene of V79 cells Mutat Res 438 1999 71 78
45. S. Schwengberg, H. Bohlen, N. Kleinsasser, K. Kehe, M. Seiss, and U.I. Walther In vitro embryotoxicity assessment with dental restorative materials J Dent 33 2005 49 55