Embryonic Stem Cells in Tissue Engineering

Handbook of Stem Cells

Volumn 1, Issue , 2004, Pages 737-746

  Levenberg, Shulamit ^a   Khademhosseini, Ali ^b   Langer, Robert ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 33645683482     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-012436643-5/50082-1     Document Type: Chapter

References (132)

1. Niklason L.E., Langer R. Prospects for organ and tissue replacement. JAMA 2001, 285:573-576.
2. Platt J.L. New directions for organ transplantation. Nature 1998, 392:11-17.
3. Petit-Zeman S. Regenerative medicine. Nat. Biotechnol. 2001, 19:201-206.
4. Shamblott M.J., Axelman J., Wang S., Bugg E.M., Littlefield J.W., Donovan P.J., Blumenthal P.D., Huggins G.R., Gearhart J.D. Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc. Natl. Acad. Sci. USA 1998, 95:13,726-13,731.
5. Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M. Embryonic stem cell lines derived from human blastocysts. Science 1998, 282:1145-1147.
6. Nerem R.M. Cellular engineering. Ann. Biomed. Eng. 1991, 19:529-545.
7. Langer R., Vacanti J.P. Tissue engineering. Science 1993, 260:920-926.
8. Till J.E., McCulloch E.A. Hemopoietic stem cell differentiation. Biochim. Biophys. Acta. 1980, 605:431-459.
9. Walter D.H., Dimmeler S. Endothelial progenitor cells: regulation and contribution to adult neovascularization. Herz. 2002, 27:579-588.
10. Edelberg J.M., Tang L., Hattori K., Lyden D., Rafii S. Young adult bone marrow-derived endothelial precursor cells restore aging-impaired cardiac angiogenic function. Circ. Res. 2002, 90:E89-E93.
11. Luttun A., Carmeliet G., Carmeliet P. Vascular progenitors: from biology to treatment. Trends Cardiovasc. Med. 2002, 12:88-96.
12. Kocher A.A., Schuster M.D., Szabolcs M.J., Takuma S., Burkhoff D., Wang J., Homma S., Edwards N.M., Itescu S. Neovascularization of ischemic myocardium by human bone marrow-derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function. Natl. Med. 2001, 7:430-436.
13. Orlic D., Kajstura J., Chimenti S., Jakoniuk I., Anderson S.M., Li B., Pickel J., McKay R., Nadal-Ginard B., Bodine D.M., Leri A., Anversa P. Bone marrow cells regenerate infarcted myocardium. Nature 2001, 410:701-705.
14. Pittenger M.F., Mackay A.M., Beck S.C., Jaiswal R.K., Douglas R., Mosca J.D., Moorman M.A., Simonetti D.W., Craig S., Marshak D.R. Multilineage potential of adult human mesenchymal stem cells. Science 1999, 284:143-147.
15. Brazelton T.R., Rossi F.M., Keshet G.I., Blau H.M. From marrow to brain: expression of neuronal phenotypes in adult mice. Science 2000, 290:1775-1779.
16. Mezey E., Chandross K.J., Harta G., Maki R.A., McKercher S.R. Turning blood into brain: Cells bearing neuronal antigens generated in vivo from bone marrow. Science 2000, 290:1779-1782.
17. Taylor D.A., Atkins B.Z., Hungspreugs P., Jones T.R., Reedy M.C., Hutcheson K.A., Glower D.D., Kraus W.E. Regenerating functional myocardium: improved performance after skeletal myoblast transplantation. Natl. Med. 1998, 4:929-933.
18. Dorfman J., Duong M., Zibaitis A., Pelletier M.P., Shum-Tim D., Li C., Chiu R.C. Myocardial tissue engineering with autologous myoblast implantation. J. Thorac. Cardiovasc. Surg. 1998, 116:744-751.
19. Storch A., Schwarz J. Neural stem cells and Parkinson's disease. J. Neurol. 2002, 249(Suppl. 3):III/30-III/32.
20. Okano H., Yoshizaki T., Shimazaki T., Sawamoto K. Isolation and transplantation of dopaminergic neurons and neural stem cells. Parkinsonism Relat. Disord. 2002, 9:23-28.
21. Fricker J. Human neural stem cells on trial for Parkinson's disease. Mol. Med. Today 1999, 5:144.
22. Bruder S.P., Jaiswal N., Ricalton N.S., Mosca J.D., Kraus K.H., Kadiyala S. Mesenchymal stem cells in osteobiology and applied bone regeneration. Clin. Orthop. 1998, 355:S247-S256. (Suppl.).
23. Palacios R., Golunski E., Samaridis J. In vitro generation of hematopoietic stem cells from an embryonic stem cell line. Proc. Natl. Acad. Sci. USA 1995, 92:7530-7534.
24. Bigas A., Martin D.I., Bernstein I.D. Generation of hematopoietic colony-forming cells from embryonic stem cells: synergy between a soluble factor from NIH-3T3 cells and hematopoietic growth factors. Blood 1995, 85:3127-3133.
25. Kaufman D.S., Hanson E.T., Lewis R.L., Auerbach R., Thomson J.A. Hematopoietic colony-forming cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA 2001, 98:10,716-10,721.
26. Hirashima M., Kataoka H., Nishikawa S., Matsuyoshi N. Maturation of embryonic stem cells into endothelial cells in an in vitro model of vasculogenesis. Blood 1999, 93:1253-1263.
27. Rohwedel J., Maltsev V., Bober E., Arnold H.H., Hescheler J., Wobus A.M. Muscle cell differentiation of embryonic stem cells reflects myogenesis in vivo: developmentally regulated expression of myogenic determination genes and functional expression of ionic currents. Dev. Biol. 1994, 164:87-101.
28. Fleischmann M., Bloch W., Kolossov E., Andressen C., Muller M., Brem G., Hescheler J., Addicks K., Fleischmann B.K. Cardiac specific expression of the green fluorescent protein during early murine embryonic development. FEBS Lett. 1998, 440:370-376.
29. Kehat I., Kenyagin-Karsenti D., Snir M., Segev H., Amit M., Gepstein A., Livne E., Binah O., Itskovitz-Eldor J., Gepstein L. Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J. Clin. Invest. 2001, 108:407-414.
30. Schuldiner M., Eiges R., Eden A., Yanuka O., Itskovitz-Eldor J., Goldstein R.S., Benvenisty N. Induced neuronal differentiation of human embryonic stem cells. Brain Res. 2001, 913:201-205.
31. Reubinoff B.E., Itsykson P., Turetsky T., Pera M.F., Reinhartz E., Itzik A., Ben-Hur T. Neural progenitors from human embryonic stem cells. Natl. Biotechnol. 2001, 19:1134-1140.
32. Zhang S.C., Wernig M., Duncan I.D., Brustle O., Thomson J.A. In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Natl. Biotechnol. 2001, 19:1129-1133.
33. Brustle O., Jones K.N., Learish R.D., Karram K., Choudhary K., Wiestler O.D., Duncan I.D., McKay R.D. Embryonic stem cell-derived glial precursors: a source of myelinating transplants. Science 1999, 285:754-756.
34. Buttery L.D., Bourne S., Xynos J.D., Wood H., Hughes F.J., Hughes S.P., Episkopou V., Polak J.M. Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells. Tissue Eng. 2001, 7:89-99.
35. Hamazaki T., Iiboshi Y., Oka M., Papst P.J., Mecham A.M., Zon L.I., Terada N. Hepatic maturation in differentiating embryonic stem cells in vitro. FEBS Lett. 2001, 497:15-19.
36. Lumelsky N., Blondel O., Laeng P., Velasco I., Ravin R., McKay R. Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets. Science 2001, 292:1389-1394.
37. Assady S., Maor G., Amit M., Itskovitz-Eldor J., Skorecki K.L., Tzukerman M. Insulin production by human embryonic stem cells. Diabetes 2001, 50:1691-1697.
38. Bjorklund L.M., Sanchez-Pernaute R., Chung S., Andersson T., Chen I.Y., McNaught K.S., Brownell A.L., Jenkins B.G., Wahlestedt C., Kim K.S., Isacson O. Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson's rat model. Proc. Natl. Acad. Sci. USA 2002, 99:2344-2349.
39. Kim J.H., Auerbach J.M., Rodriguez-Gomez J.A., Velasco I., Gavin D., Lumelsky N., Lee S.H., Nguyen J., Sanchez-Pernaute R., Bankiewicz K., McKay R. Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease. Nature 2002, 418:50-56.
40. Yang Y., Min J.Y., Rana J.S., Ke Q., Cai J., Chen Y., Morgan J.P., Xiao Y.F. VEGF enhances functional improvement of postinfarcted hearts by transplantation of ESC-differentiated cells. J. Appl. Physiol. 2002, 93:1140-1151.
41. Klug M.G., Soonpaa M.H., Koh G.Y., Field L.J. Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts. J. Clin. Invest. 1996, 98:216-224.
42. Min J.Y., Yang Y., Converso K.L., Liu L., Huang Q., Morgan J.P., Xiao Y.F. Transplantation of embryonic stem cells improves cardiac function in postinfarcted rats. J. Appl. Physiol. 2002, 92:288-296.
43. Mummery C., Ward-van Oostwaard D., Doevendans P., Spijker R., van den Brink S., Hassink R., van der Heyden M., Opthof T., Pera M., de la Riviere A.B., Passier R., Tertoolen L. Differentiation of human embryonic stem cells to cardiomyocytes: Role of coculture with visceral endoderm-like cells. Circulation 2003, 107:2733-2740.
44. Kehat I., Gepstein A., Spira A., Itskovitz-Eldor J., Gepstein L. High-resolution electrophysiological assessment of human embryonic stem cell-derived cardiomyocytes: a novel in vitro model for the study of conduction. Circ. Res. 2002, 91:659-661.
45. Soria B., Roche E., Berna G., Leon-Quinto T., Reig J.A., Martin F. Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice. Diabetes 2000, 49:157-162.
46. Yamashita J., Itoh H., Hirashima M., Ogawa M., Nishikawa S., Yurugi T., Naito M., Nakao K. Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors. Nature 2000, 408:92-96.
47. Levenberg S., Golub J.S., Amit M., Itskovitz-Eldor J., Langer R. Endothelial cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA 2002, 99:4391-4396.
48. Uludag H., De Vos P., Tresco P.A. Technology of mammalian cell encapsulation. Adv. Drug Deliv. Rev. 2000, 42:29-64.
49. Vacanti J.P., Langer R. Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation. Lancet. 1999, 354(Suppl. 1):SI32-SI34.
50. Lanza R.P., Langer R.S., Chick W.L. Principles of tissues engineering 1997, Academic Press/R.G. Landes, Berlin.
51. Langer R.S., Vacanti J.P. Tissue engineering: the challenges ahead. Sci. Am. 1999, 280:86-89.
52. Laurencin C.T., Attawia M.A., Elgendy H.E., Herbert K.M. Tissue-engineered bone regeneration using degradable polymers: the formation of mineralized matrices. Bone 1996, 19:93S-99S.
53. Mooney D.J., Baldwin D.F., Suh N.P., Vacanti J.P., Langer R. Novel approach to fabricate porous sponges of poly (D, L-lactic-co-glycolic acid) without the use of organic solvents. Biomaterials 1996, 17:1417-1422.
54. He S., Yaszemski M.J., Yasko A.W., Engel P.S., Mikos A.G. Injectable biodegradable polymer composites based on poly(propylene furmate) crosslinked with poly(ethylene glycol)-dimethacrylate. Biomaterials 2000, 21:2389-2394.
55. Mooney D.J., Mazzoni C.L., Breuer C., McNamara K., Hern D., Vacanti J.P., Langer R. Stabilized polyglycolic acid fibre-based tubes for tissue engineering. Biomaterials 1996, 17:115-124.
56. Friedman C.D., Costantino P.D. Hydroxyapatite cement, a smart biomaterial for craniofacial skeletal tissue engineering. Surg. Technol. Int. 1998, 7:421-423.
57. Pelissier P., Villars F., Mathoulin-Pellissier S., Bareille R., Lafage-Proust M.H., Vilamitjana-Amedee J. Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats. Plast. Reconstr. Surg. 2003, 111:1932-1941.
58. Li S.H., De Wijn J.R., Layrolle P., de Groot K. Synthesis of macroporous hydroxyapatite scaffolds for bone tissue engineering. J. Biomed. Mater. Res. 2002, 61:109-120.
59. LeGeros R.Z. Properties of osteoconductive biomaterials: calcium phosphates. Clin. Orthop. 2002, 395:81-98.
60. Kreklau B., Sittinger M., Mensing M.B., Voigt C., Berger G., Burmester G.R., Rahmanzadeh R., Gross U. Tissue engineering of biphasic joint cartilage transplants. Biomaterials 1999, 20:1743-1749.
61. Chevallay B., Herbage D. Collagen-based biomaterials as 3D scaffold for cell cultures: applications for tissue engineering and gene therapy. Med. Biol. Eng. Comput. 2000, 38:211-218.
62. Rowley J.A., Madlambayan G., Mooney D.J. Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 1999, 20:45-53.
63. Ohgushi H., Miyake J., Tateishi T. Mesenchymal stem cells and bioceramics: strategies to regenerate the skeleton. Novartis. Found. Symp. 2003, 249:118-127. discussion 127-132, 170-174, 239-241.
64. Cancedda R., Mastrogiacomo M., Bianchi G., Derubeis A., Muraglia A., Quarto R. Bone marrow stromal cells and their use in regenerating bone. Novartis. Found. Symp. 2003, 249:133-143. discussion 143-147, 170-174, 239-241.
65. Sherwood J.K., Riley S.L., Palazzolo R., Brown S.C., Monkhouse D.C., Coates M., Griffith L.G., Landeen L.K., Ratcliffe A. A three-dimensional osteochondral composite scaffold for articular cartilage repair. Biomaterials 2002, 23:4739-4751.
66. Baek C.H., Lee J.C., Jung Y.G., Ko Y.J., Yoon J.J., Park T.G. Tissue-engineered cartilage on biodegradable macroporous scaffolds: cell shape and phenotypic expression. Laryngoscope 2002, 112:1050-1055.
67. Rahman M.S., Tsuchiya T. Enhancement of chondrogenic differentiation of human articular chondrocytes by biodegradable polymers. Tissue Eng. 2001, 7:781-790.
68. Niklason L.E., Langer R.S. Advances in tissue engineering of blood vessels and other tissues. Transplant. Immunol. 1997, 5:303-306.
69. Niklason L.E., Gao J., Abbott W.M., Hirschi K.K., Houser S., Marini R., Langer R. Functional arteries grown in vitro. Science 1999, 284:489-493.
70. Shinoka T., Shum-Tim D., Ma P.X., Tanel R.E., Isogai N., Langer R., Vacanti J.P., Mayer J.E. Creation of viable pulmonary artery autografts through tissue engineering. J. Thorac. Cardiovasc. Surg. 1998, 115:536-545. discussion 545-546.
71. Kim B.S., Nikolovski J., Bonadio J., Smiley E., Mooney D.J. Engineered smooth muscle tissues: regulating cell phenotype with the scaffold. Exp. Cell Res. 1999, 251:318-328.
72. Cui W., Kim D.H., Imamura M., Hyon S.H., Inoue K. Tissue-engineered pancreatic islets: culturing rat islets in the chitosan sponge. Cell Transplant. 2001, 10:499-502.
73. Hasirci V., Berthiaume F., Bondre S.P., Gresser J.D., Trantolo D.J., Toner M., Wise D.L. Expression of liver-specific functions by rat hepatocytes seeded in treated poly(lactic-coglycolic) acid biodegradable foams. Tissue Eng. 2001, 7:385-394.
74. Young C.S., Terada S., Vacanti J.P., Honda M., Bartlett J.D., Yelick P.C. Tissue engineering of complex tooth structures on biodegradable polymer scaffolds. J. Dent. Res. 2002, 81:695-700.
75. Lu L., Yaszemski M.J., Mikos A.G. Retinal pigment epithelium engineering using synthetic biodegradable polymers. Biomaterials 2001, 22:3345-3355.
76. Badiavas E.V., Paquette D., Carson P., Falanga V. Human chronic wounds treated with bioengineered skin: histologic evidence of host-graft interactions. J. Am. Acad. Dermatol. 2002, 46:524-530.
77. Herson M.R., Mathor M.B., Altran S., Capelozzi V.L., Ferreira M.C. In vitro construction of a potential skin substitute through direct human keratinocyte plating onto decellularized glycerol-preserved allodermis. Artif. Organs 2001, 25:901-906.
78. Griffith L.G., Naughton G. Tissue engineering-current challenges and expanding opportunities. Science 2002, 295:1009-1014.
79. Oberpenning F., Meng J., Yoo J.J., Atala A. De novo reconstitution of a functional mammalian urinary bladder by tissue engineering. Natl. Biotechnol. 1999, 17:149-155.
80. Weber M., Steinert A., Jork A., Dimmler A., Thurmer F., Schutze N., Hendrich C., Zimmerman U. Formation of cartilage matrix proteins by BMP-transfected murine mesenchymal stem cells encapsulated in a novel class of alginates. Biomaterials 2002, 23:2003-2013.
81. Boo J.S., Yamada Y., Okazaki Y., Hibino Y., Okada K., Hata K., Yoshikawa T., Sugiura Y., Ueda M. Tissue-engineered bone using mesenchymal stem cells and a biodegradable scaffold. J. Craniofac. Surg. 2002, 13:231-239. discussion 240-243.
82. Howard D., Partridge K., Yang X., Clarke N.M., Okubo Y., Bessho K., Howdle S.M., Shakesheff K.M., Oreffo R.O. Immunoselection and adenoviral genetic modulation of human osteoprogenitors: in vivo bone formation on PLA scaffold. Biochem. Biophys. Res. Commun. 2002, 299:208-215.
83. Yamada Y., Boo J.S., Ozawa R., Nagasaka T., Okazaki Y., Hata K., Ueda M. Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold. J. Craniomaxillofac. Surg. 2003, 31:27-33.
84. Bensaid W., Triffitt J.T., Blanchat C., Oudina K., Sedel L., Petite H. A biodegradable fibrin scaffold for mesenchymal stem cell transplantation. Biomaterials 2003, 24:2497-2502.
85. Park K.I., Teng Y.D., Snyder E.Y. The injured brain interacts reciprocally with neural stem cells supported by scaffolds to reconstitute lost tissue. Natl. Biotechnol. 2002, 20:1111-1117.
86. Suh H., Song M.J., Park Y.N. Behavior of isolated rat oval cells in porous collagen scaffold. Tissue Eng. 2003, 9:411-420.
87. Lavik E.B., Hrkach J.S., Lotan N., Nazarov R., Langer R. A simple synthetic route to the formation of a block copolymer of poly(lactic-co-glycolic acid) and polylysine for the fabrication of functionalized, degradable structures for biomedical applications. J. Biomed. Mater. Res. 2001, 58:291-294.
88. Lavik E., Teng Y.D., Snyder E., Langer R. Seeding neural stem cells on scaffolds of PGA, PLA, and their copolymers. Methods Mol. Biol. 2002, 198:89-97.
89. Teng Y.D., Lavik E.B., Qu X., Park K.I., Ourednik J., Zurakowski D., Langer R., Snyder E.Y. Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proc. Natl. Acad. Sci. USA 2002, 99:3024-3029.
90. Martin I., Shastri V.P., Padera R.F., Yang J., Mackay A.J., Langer R., Vunjak-Novakovic G., Freed L.E. Selective differentiation of mammalian bone marrow stromal cells cultured on three-dimensional polymer foams. J. Biomed. Mater. Res. 2001, 55:229-235.
91. Levenberg S., Huang N.F., Lavik E., Rogers A.B., Itskovitz-Eldor J., Langer R. Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds. Proc. Natl. Acad. Sci. U. S. A. 2003, 100:12741-12746.
92. Lim F., Sun A.M. Microencapsulated islets as a bioartificial endocrine pancreas. Science 1980, 210:908-910.
93. Sefton M.V., May M.H., Lahooti S., Babensee J.E. Making microencapsulation work: conformal coating, immobilization gels, and in vivo performance. J. Control Release 2000, 65:173-186.
94. Zekorn T., Siebers U., Horcher A., Schnettler R., Zimmermann U., Bretzel R.G., Federlin K. Alginate coating of islets of Langerhans: in vitro studies on a new method for microencapsulation for immunoisolated transplantation. Acta. Diabetol. 1992, 29:41-45.
95. Chicheportiche D., Reach G. In vitro kinetics of insulin release by microencapsulated rat islets: effect of the size of the microcapsules. Diabetologia 1988, 31:54-57.
96. Uludag H., Sefton M.V. Microencapsulated human hepatoma (HepG2) cells: in vitro growth and protein release. J. Biomed. Mater. Res. 1993, 27:1213-1224.
97. Wang L., Sun J., Li L., Harbour C., Mears D., Koutalistras N., Sheil A.G. Factors affecting hepatocyte viability and CYPIA1 activity during encapsulation. Artif. Cells Blood Substit. Immobil. Biotechnol. 2000, 28:215-227.
98. Chandy T., Mooradian D.L., Rao G.H. Evaluation of modified alginate-chitosan-polyethylene glycol microcapsules for cell encapsulation. Artif. Organs 1999, 23:894-903.
99. Wang Y., Wang S.D., Lin S.Z., Chiou A.L., Chen L.K., Chen J.F., Zhou F.C. Transplantation of microencapsulated PC12 cells provides long-term improvement of dopaminergic functions. Chin. J. Physiol. 1997, 40:121-129.
100. Aebischer P., Wahlberg L., Tresco P.A., Winn S.R. Macroencapsulation of dopamine-secreting cells by coextrusion with an organic polymer solution. Biomaterials 1991, 12:50-56.
101. Esposito E., Cortesi R., Nastruzzi C. Gelatin microspheres: influence of preparation parameters and thermal treatment on chemico-physical and biopharmaceutical properties. Biomaterials 1996, 17:2009-2020.
102. Vallbacka J.J., Nobrega J.N., Sefton M.V. Tissue engineering as a platform for controlled release of therapeutic agents: implantation of microencapsulated dopamine producing cells in the brains of rats. J. Control Release 2001, 72:93-100.
103. Soria B., Roche E., Berna G., Leon-Quinto T., Reig J.A., Martin F. Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice. Diabetes 2000, 49:157-162.
104. Ramalho-Santos M., Yoon S., Matsuzaki Y., Mulligan R.C., Melton D.A. "Stemness": transcriptional profiling of embryonic and adult stem cells. Science 2002, 298:597-600.
105. Ivanova N.B., Dimos J.T., Schaniel C., Hackney J.A., Moore K.A., Lemischka I.R. A stem cell molecular signature. Science 2002, 298:601-604.
106. Phillips R.L., Ernst R.E., Brunk B., Ivanova N., Mahan M.A., Deanehan J.K., Moore K.A., Overton G.C., Lemischka I.R. The genetic program of hematopoietic stem cells. Science 2000, 288:1635-1640.
107. Rajagopal J., Anderson W.J., Kume S., Martinez O.I., Melton D.A. Insulin staining of ES cell progeny from insulin uptake. Science 2003, 299:363.
108. Eiges R., Schuldiner M., Drukker M., Yanuka O., Itskovitz-Eldor J., Benvenisty N. Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells. Curr. Biol. 2001, 11:514-518.
109. Zwaka T.P., Thomson J.A. Homologous recombination in human embryonic stem cells. Natl. Biotechnol. 2003, 21:319-321.
110. Chen Y., Li X., Eswarakumar V.P., Seger R., Lonai P. Fibroblast growth factor (FGF) signaling through PI 3-kinase and Akt/PKB is required for embryoid body differentiation. Oncogene 2000, 19:3750-3756.
111. Itskovitz-Eldor J., Schuldiner M., Karsenti D., Eden A., Yanuka O., Amit M., Soreq H., Benvenisty N. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol. Med. 2000, 6:88-95.
112. Magyar J.P., Nemir M., Ehler E., Suter N., Perriard J.C., Eppenberger H.M. Mass production of embryoid bodies in microbeads. Ann. NY Acad. Sci. 2001, 944:135-143.
113. Guan K., Furst D.O., Wobus A.M. Modulation of sarcomere organization during embryonic stem cell-derived cardiomyocyte differentiation. Eur. J. Cell Biol. 1999, 78:813-823.
114. Klinz F., Bloch W., Addicks K., Hescheler J. Inhibition of phosphatidylinositol-3-kinase blocks development of functional embryonic cardiomyocytes. Exp. Cell Res. 1999, 247:79-83.
115. Maltsev V.A., Ji G.J., Wobus A.M., Fleischmann B.K., Hescheler J. Establishment of β-adrenergic modulation of L-type Ca2+ current in the early stages of cardiomyocyte development. Circ. Res. 1999, 84:136-145.
116. Dang S.M., Kyba M., Perlingeiro R., Daley G.Q., Zandstra P.W. Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems. Biotechnol. Bioeng. 2002, 78:442-453.
117. Wartenberg M., Gunther J., Hescheler J., Sauer H. The embryoid body as a novel in vitro assay system for antiangiogenic agents. Lab Invest. 1998, 78:1301-1314.
118. Carrier R.L., Rupnick M., Langer R., Schoen F.J., Freed L.E., Vunjak-Novakovic G. Perfusion improves tissue architecture of engineered cardiac muscle. Tissue Eng. 2002, 8:175-188.
119. Davisson T., Sah R.L., Ratcliffe A. Perfusion increases cell content and matrix synthesis in chondrocyte three-dimensional cultures. Tissue Eng. 2002, 8:807-816.
120. Vunjak-Novakovic G., Martin I., Obradovic B., Treppo S., Grodzinsky A.J., Langer R., Freed L.E. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. J. Orthop. Res. 1999, 17:130-138.
121. Martin I., Obradovic B., Treppo S., Grodzinsky A.J., Langer R., Freed L.E., Vunjak-Novakovic G. Modulation of the mechanical properties of tissue-engineered cartilage. Biorheology 2000, 37:141-147.
122. Botchwey E.A., Pollack S.R., Levine E.M., Laurencin C.T. Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system. J. Biomed. Mater. Res. 2001, 55:242-253.
123. Margolis L., Hatfill S., Chuaqui R., Vocke C., Emmert-Buck M., Linehan W.M., Duray P.H. Long term organ culture of human prostate tissue in a NASA-designed rotating wall bioreactor. J. Urol. 1999, 161:290-297.
124. Freed L.E., Langer R., Martin I., Pellis N.R., Vunjak-Novakovic G. Tissue engineering of cartilage in space. Proc. Natl. Acad. Sci. USA 1997, 94:13,885-13,890.
125. Li C., Xu Q. Mechanical stress-initiated signal transductions in vascular smooth muscle cells. Cell Signal 2000, 12:435-445.
126. Murphy W.L., Peters M.C., Kohn D.H., Mooney D.J. Sustained release of vascular endothelial growth factor from mineralized poly(lactide-co-glycolide) scaffolds for tissue engineering. Biomaterials 2000, 21:2521-2527.
127. Richardson T.P., Peters M.C., Ennett A.B., Mooney D.J. Polymeric system for dual growth factor delivery. Natl. Biotechnol. 2001, 19:1029-1034.
128. Cook A.D., Hrkach J.S., Gao N.N., Johnson I.M., Pajvani U.B., Cannizzaro S.M., Langer R. Characterization and development of RGD-peptide-modified poly(lactic acid-co-lysine) as an interactive, resorbable biomaterial. J. Biomed. Mater. Res. 1997, 35:513-523.
129. Lanza R.P., Cibelli J.B., Blackwell C., Cristofalo V.J., Francis M.K., Baerlocher G.M., Mak J., Schertzer M., Chavez E.A., Sawyer N., Lansdorp P.M., West M.D. Extension of cell life span and telomere length in animals cloned from senescent somatic cells. Science 2000, 288:665-669.
130. Bhatia S.N., Yarmush M.L., Toner M. Controlling cell interactions by micropatterning in cocultures: hepatocytes and 3T3 fibroblasts. J. Biomed. Mater. Res. 1997, 34:189-199.
131. Bhatia S.N., Balis U.J., Yarmush M.L., Toner M. Probing heterotypic cell interactions: Hepatocyte function in microfabricated cocultures. J. Biomater. Sci. Polym. Ed. 1998, 9:1137-1160.
132. Bhatia S.N., Balis U.J., Yarmush M.L., Toner M. Microfabrication of hepatocytes-fibroblast cocultures: role of homotypic cell interactions. Biotechnol. Prog. 1998, 14.