Development: Insulin staining of ES cell progeny from insulin uptake

Science

Volumn 299, Issue 5605, 2003, Pages 363-

  Rajagopal, Jayaraj ^a   Anderson, William J ^a   Kume, Shoen ^a   Martinez, Olga I ^a   Melton, Douglas A ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE; INSULIN; MESSENGER RNA; ANTIBODY;

DEVELOPMENTAL BIOLOGY;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; EMBRYO; EMBRYO CELL; GENE EXPRESSION; MOUSE; NONHUMAN; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; PROGENY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; APOPTOSIS; CELL LINE; CONFOCAL MICROSCOPY; CYTOLOGY; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM; PANCREAS ISLET; STEM CELL;

ANIMALIA;

ANIMALS; ANTIBODIES; APOPTOSIS; CELL DIFFERENTIATION; CELL LINE; EMBRYO; HUMANS; INSULIN; ISLETS OF LANGERHANS; MICE; MICROSCOPY, CONFOCAL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; STEM CELLS;

EID: 0037449573     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. N. Lumelsky et al., Science 292, 1389 (2001).
2. S. Assady et al., Diabetes 50, 1691 (2001).
3. B. Soria et al., Diabetes 49, 157 (2001).
4. A. Shiroi et al., Stem Cells 20, 284 (2002).
5. Y. Hori et al., Proc. Natl. Acad. Sci. U.S.A. 99, 16105 (2002).
6. V. K. Ramiya et al., Nature Med. 6, 278 (2000).
7. S. Bonner-Weir et al., Proc. Natl. Acad. Sci. U.S.A. 97, 7999 (2000).
8. H. Zulewski et al., Diabetes 50, S21 (2001).
9. N. Benvenisty, O. Yanuka, personal communication.
10. We thank N. Lumelsky and R. McKay for help in reproducing their culture regimen (1) and for comments on the manuscript. We thank R. Perak. N. Benvenisty, and members of the Melton lab. The following individuals kindly provided reagents: C. Wright (R1 Pdx1-lacZ ES cells), D. Bucchini (Ins2-lacZ ES cells). A. McMahon (AV3 ES cells), R. McKay (nestin antibody, E14.1 ES cells), and J. Itskovitz-Eldor (H9 human ES cells). A detailed protocol is available upon request.