Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets

Science

Volumn 292, Issue 5520, 2001, Pages 1389-1394

  Lumelsky, Nadya ^a   Blondel, Olivier ^a,c   Laeng, Pascal ^b,d   Velasco, Ivan ^a   Ravin, Rea ^a   McKay, Ron ^a  

^a NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

^b NATIONAL INSTITUTE OF MENTAL HEALTH   (United States)

^c GLAXOSMITHKLINE   (United States)

^d Psychiatric Genomics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DISEASES; GLUCOSE; INSULIN; MEDICAL IMAGING; NEUROLOGY; TOPOLOGY;

VASCULARIZATION;

CELLS;

GLUCOSE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL DIFFERENTIATION; CELL TYPE; CONTROLLED STUDY; DIABETES MELLITUS; EMBRYO; EMBRYO DEVELOPMENT; EMBRYONIC STRUCTURES; INSULIN METABOLISM; INSULIN RELEASE; INSULIN SYNTHESIS; MOUSE; NONHUMAN; PANCREAS ISLET CELL; PATHOGENESIS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; STEM CELL; VASCULARIZATION;

ANIMALS; BIOLOGICAL MARKERS; CALCIUM; CALCIUM SIGNALING; CELL AGGREGATION; CELL DIFFERENTIATION; CELL DIVISION; CYCLIC AMP; DIABETES MELLITUS, EXPERIMENTAL; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; GLUCOSE; HUMANS; INSULIN; ISLETS OF LANGERHANS; ISLETS OF LANGERHANS TRANSPLANTATION; MICE; NEURONS; POTASSIUM; RNA, MESSENGER; STEM CELLS;

ANIMALIA;

EID: 0035906902     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1058866     Document Type: Article

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48. We thank T. Doetschman for gift of E14.1 ES cells and A. Nagy for gift of B5 ES cells. We also thank C. Smith for her help with confocal microscopy and J.-H. Kim for alkaline phosphatase analysis of ES cells. I.V. was supported by a fellowship from the PEW charitable Trust. We thank the National Parkinsons Foundation for their support of our work and C. Gerfen for many positive contributions.