Directed evolution of epoxide hydrolase from A. radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling

Chemistry and Biology

Volumn 11, Issue 7, 2004, Pages 981-990

  Van Loo, Bert ^a   Spelberg, Jeffrey H Lutje ^a   Kingma, Jaap ^a   Sonke, Theo ^b   Wubbolts, Marcel G ^b   Janssen, Dick B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b DSM Pharmaceutical Products   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

EPOXIDE HYDROLASE; PRIMER DNA;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METHODOLOGY; MUTATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RHIZOBIUM; SITE DIRECTED MUTAGENESIS; STEREOISOMERISM;

BASE SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; EPOXIDE HYDROLASES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; POLYMERASE CHAIN REACTION; RHIZOBIUM; STEREOISOMERISM;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; PROKARYOTA; RHIZOBIUM;

EID: 3342911436     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chembiol.2004.04.019     Document Type: Article

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