Learning from directed evolution: Theoretical investigations into cooperative mutations in lipase enantioselectivity

ChemBioChem

Volumn 5, Issue 2, 2004, Pages 214-223

  Bocola, Marco ^a   Otte, Nikolaj ^a   Jaeger, Karl Erich ^b   Reetz, Manfred T ^a   Thiel, Walter ^a  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Directed evolution; Enantioselectivity; Enzyme catalysis; Molecular dynamics; Molecular modeling

Indexed keywords

ALPHA METHYLDECANOIC ACID; AMINO ACID DERIVATIVE; DECANOIC ACID; ESTER; METHYL GROUP; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

BACTERIAL MUTATION; BINDING AFFINITY; CATALYSIS; CATALYST; COMPLEX FORMATION; CONFERENCE PAPER; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENANTIOMER; ENANTIOSELECTIVITY; ENERGY YIELD; ENZYME MECHANISM; ENZYME SUBSTRATE; GEOMETRY; HYDROGEN BOND; HYDROLYSIS; INTERMETHOD COMPARISON; MOLECULAR DYNAMICS; MOLECULAR EVOLUTION; MOLECULAR INTERACTION; MOLECULAR MECHANICS; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SIMULATION; STEREOCHEMISTRY; STRUCTURE ANALYSIS; TECHNIQUE; WILD TYPE;

CATALYSIS; COMPUTER SIMULATION; CRYSTALLOGRAPHY, X-RAY; DIRECTED MOLECULAR EVOLUTION; ESTERS; HYDROLYSIS; KINETICS; LIPASE; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTATION; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS AERUGINOSA; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 3042531902     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/cbic.200300731     Document Type: Conference Paper

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