Production of enantiopure styrene oxide by recombinant Escherichia coli synthesizing a two-component styrene monooxygenase

Biotechnology and Bioengineering

Volumn 69, Issue 1, 2000, Pages 91-100

  Panke, Sven ^a,b   Wubbolts, Marcel G ^a,c   Schmid, Andreas ^a   Witholt, Bernard ^a  

^a ETH ZURICH   (Switzerland)

^b DSM RESEARCH   (Netherlands)

^c DSM RESOLVE   (Netherlands)

Author keywords

Enantiopure building blocks; Recombinant biocatalysis; Two liquid phase culture

Indexed keywords

BATCH CELL CULTURE; BIOCATALYSTS; ESCHERICHIA COLI; GENES; GLUCOSE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MAGNESIUM COMPOUNDS; OXIDATION; OXIDES; SOLVENTS; SYNTHESIS (CHEMICAL); YEAST;

APOLAR CARRIER SOLVENT; ENANTIOPURE BUILDING BLOCKS; MAGNESIUM SULFATE; PHASE FRACTION; PLASMID; RECOMBINANT BIOCATALYSIS; SHAKE FLASK EXPERIMENTS; STYRENE MONOOXYGENASE; STYRENE OXIDE; TWO LIQUID PHASE FED BATCH PROCESS;

STYRENE;

STYRENE OXIDE; UNSPECIFIC MONOOXYGENASE;

ARTICLE; BIOENGINEERING; CATALYSIS; ENANTIOMER; ENZYME MECHANISM; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; NONHUMAN; OXIDATION; PARTITION COEFFICIENT; PSEUDOMONAS; STEREOSPECIFICITY;

BASE SEQUENCE; DNA PRIMERS; EPOXY COMPOUNDS; ESCHERICHIA COLI; FERMENTATION; OXYGENASES; RECOMBINATION, GENETIC; STEREOISOMERISM;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS OLEOVORANS; PSEUDOMONAS SP.;

EID: 0034609021     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000705)69:1<91::AID-BIT11>3.0.CO;2-X     Document Type: Article

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