Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM)

Journal of the American Chemical Society

Volumn 125, Issue 38, 2003, Pages 11476-11477

  DeSantis, Grace ^a   Wong, Kelvin ^a   Farwell, Bob ^a   Chatman, Kelly ^a   Zhu, Zoulin ^a   Tomlinson, Geoff ^a   Huang, Hongjun ^a   Tan, Xuqiu ^a   Bibbs, Lisa ^a   Chen, Pei ^a   Kretz, Keith ^a   Burk, Mark J ^a  

^a DIVERSA CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 HYDROXYGLUTARYL NITRILE; 4 CYANO 3 HYDROXYBUTYRIC ACID; ATORVASTATIN; BUTYRIC ACID DERIVATIVE; HYDROXYNITRILE LYASE; NITRILASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; DNA DETERMINATION; DNA LIBRARY; DRUG SYNTHESIS; ENANTIOSELECTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE SITE SATURATION MUTAGENESIS; HYDROLYSIS; MUTAGEN TESTING; NONHUMAN; REACTION ANALYSIS;

AMINO ACID SUBSTITUTION; AMINOHYDROLASES; HYDROXYBUTYRATES; MUTAGENESIS, SITE-DIRECTED; STEREOISOMERISM;

EID: 0141645603     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja035742h     Document Type: Article

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