Variations in the sequence and expression of the Plasmodium falciparum chloroquine resistance transporter (Pfcrt) and their relationship to chloroquine resistance in vitro

Molecular and Biochemical Parasitology

Volumn 136, Issue 2, 2004, Pages 273-285

  Durrand, Valérie ^a   Berry, Antoine ^b   Sem, Rithy ^a,c   Glaziou, Philippe ^a   Beaudou, Joelle ^b   Fandeur, Thierry ^a  

^a INSTITUT PASTEUR DU CAMBODGE   (Cambodia)

^b UNIVERSITÉ DE TOULOUSE   (France)

^c National Malaria Center   (Cambodia)

Author keywords

50 inhibitory concentration; chloroquine; chloroquine resistance; chloroquine susceptibility; CQ; CQR; CQS; Ct; IC50 ; PCR; Pfcrt gene; Plasmodium falciparum; polymerase chain reaction; real time polymerase chain reaction; RT PCR; threshold cycle

Indexed keywords

CARRIER PROTEIN; CHLOROQUINE;

ARTICLE; DNA SEQUENCE; DRUG SENSITIVITY; GENE AMPLIFICATION; GENE MUTATION; HAPLOTYPE; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PHYLOGENY; PLASMODIUM FALCIPARUM; POINT MUTATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; ANTIMALARIALS; BASE SEQUENCE; BIOLOGICAL TRANSPORT, ACTIVE; CAMBODIA; CHLOROQUINE; DNA, COMPLEMENTARY; DNA, PROTOZOAN; DRUG RESISTANCE; GENE EXPRESSION; GENES, PROTOZOAN; HAPLOTYPES; HUMANS; MALARIA, FALCIPARUM; PLASMODIUM FALCIPARUM; POLYMORPHISM, GENETIC; PROTOZOAN PROTEINS; VARIATION (GENETICS);

PLASMODIUM FALCIPARUM;

EID: 2942622405     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2004.03.016     Document Type: Article

References (45)

1. WHO Expert Committee on Malaria. WHO technical report Series 2000 892 (WHO Genova).
2. Payne D. Spread of chloroquine resistance in Plasmodium falciparum. Parasitol. Today. 3:1987;241-246
3. Su X.-z., Kirkman L.A., Fujioka H., Wellems T.E. Complex polymorphisms in a ∼ 330 kDa protein are linked to chloroquine-resistant P. falciparum in Southeast Asia and Africa. Cell. 91:1997;593-603
4. Fidock D.A., Nomura T., Talley A.K., et al. Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance. Mol Cell. 6:2000;861-871
5. Warhurst D.C. A molecular marker for chloroquine-resistant falciparum malaria. N. Engl. J. Med. 344:2001;299-302
6. Djimde A., Doumbo O.K., Steketee R.W., Plowe C.V. Application of a molecular marker for surveillance of chloroquine-resistant falciparum malaria. Lancet. 358:2001;890-891
7. Sidhu A.B., Verdier-Pinard D., Fidock D.A. Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by Pfcrt mutations. Science. 298:2002;210-213
8. Babiker H.A., Pringle S.J., Abdel-Muhsin A., Mackinnon M., Hunt P., Walliker D. High-level chloroquine resistance in Sudanese isolates of Plasmodium falciparum is associated with mutations in the chloroquine resistance transporter gene pfcrt and the multidrug resistance Gene pfmdr1. J. Infect. Dis. 183:2001;1535-1538
9. Basco L.K., Ringwald P. Analysis of the key pfcrt point mutation and in vitro and in vivo response to chloroquine in Yaounde. Cameroon J. Infect. Dis. 183:2001;1828-1831
10. Djimde A., Doumbo O.K., Cortese J.F., et al. A molecular marker for chloroquine-resistant falciparum malaria. N. Engl. J. Med. 344:2001;257-263
11. Dorsey G., Kamya M.R., Singh A., Rosenthal P.J. Polymorphisms in the Plasmodium falciparum pfcrt and pfmdr-1 genes and clinical response to chloroquine in Kampala, Uganda. J. Infect. Dis. 183:2001;1417-1420
12. Mayor A.G., Gomez-Olive X., Aponte J.J., et al. Prevalence of the K76T mutation in the putative Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene and its relation to chloroquine resistance in Mozambique. J. Infect. Dis. 183:2001;1413-1416
13. Pillai D.R., Labbe A.C., Vanisaveth V., et al. Plasmodium falciparum malaria in Laos: chloroquine treatment outcome and predictive value of molecular markers. J. Infect. Dis. 183:2001;789-795
14. Lim P., Chy S., Ariey F., et al. Pfcrt polymorphism and chloroquine resistance in Plasmodium falciparum strains isolated in Cambodia. Antimicrob. Agents Chemother. 47:2003;87-94
15. Vieira P.P., Das Gracas Alecrim M., Da Silva L.H., Gonzalez-Jimenez I., Zalis M.G. Analysis of the PfCRT K76T mutation in Plasmodium falciparum isolates from the Amazon region of Brazil. J. Infect. Dis. 183:2001;1832-1833
16. Macreadie I., Ginsburg H., Sirawaraporn W., Tilley L. Antimalarial drug development and new targets. Parasitol. today. 16:2000;438-444
17. Desjardins R.E., Canfield C.J., Haynes J.D., Chulay J.D. Quantitative assessment of antimalarial activity in vitro by an automated microdilution technique. Antimicrob. Agents Chemother. 16:1979;710-718
18. Le Bras J., Deloron P. In vitro study of drug sensitivity of Plasmodium falciparum: evaluation of a new semi-micro test. Am. J. Trop. Med. Hyg. 32:1983;447-451
19. Ringwald P., Basco L.K. Comparison of in vivo and in vitro tests of resistance in patients treated with chloroquine in Yaounde, Cameroon. Bull. World Health Organ. 77:1999;34-43
20. Mercereau-Puijalon O., Fandeur T., Bonnefoy S., Jacquemot C., Sarthou J.L. A study of a genomic diversity of Plasmodium falciparum in Senegal. 2. Typing by the use of the polymerase chain reaction. Acta Trop. 49:1991;293-304
21. Overbergh I., Giulietti D., Valckx B., Decallonne B., Bouillon R., Mathieu C. The use of RT reverse transcriptase PCR for the quantification of cytokine gene expression. J. Biomol. Tech. 14:2003;33-43
22. Bustin S.A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J. Mol. Endocrinol. 29:2002;23-39
23. McCutchan T.F., De la Cruz V.F., Lal A.A., Gunderson J.H., Elwood H.J., Sogin M.L. Primary sequences of two small subunit ribosomal RNA genes from Plasmodium falciparum. Mol. Biochem. Parasitol. 28:1988;63-68
24. Hermsen C.C., Telgt D.S.C., Linders E.H.P., et al. Detection of Plasmodium falciparum malaria parasites in vivo by real-time quantitative PCR. Mol. Biochem. Parasitol. 118:2001;247-251
25. Blair P.L., Witney A., Haynes J.D., Moch J.K., Carucci D.J., Adams J.H. Transcripts of developmentally regulated Plasmodium falciparum genes quantified by real-time RT-PCR. Nucleic Acids Res. 30:2002;2224-2231
26. Schmittgen T.D., Zakrajsek B.A., Mills A.G., Gorn V., Singer M.J., Reed M.W. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Anal. Biochem. 285:2000;194-204
27. -ΔΔCt method. Methods. 25:2001;402-408
28. Holder A.A., Lockyer M.J., Odink K.J., et al. Primary structure of the precursor to the three major antigens of Plasmodium falciparum merozoïtes. Nature. 317:1985;270-273
29. Smythe J.A., Peterson M.G., Coppel R.L., Saul A.J., Kemp D.J., Anders R.F. Structural diversity in the 45-kilodalton merozoïte surface antigen of Plasmodium falciparum. Mol. Biochem. Parasitol. 39:1990;227-234
30. Chen N., Kyles D.E., Pasay C., et al. Pfcrt allelic types with two novel amino acid mutations in chloroquine resistant Plasmodium falciparum isolates from philippines. Antimicrob. Agents Chemother. 47:2003;3500-3505
31. Thaithong S., Ranford-Cartwright L.C., Siripoon N., et al. Plasmodium falciparum: genes mutations and amplification of dihydrofolate reductase genes in parasites grown in vitro in presence of pyrimethamine. Exp. Parasitol. 98:2001;59-70
32. Eyles D.E., Hoo C.C., Warren M.C., Sandosham A.A. Plasmodium falciparum resistant to chloroquine in Cambodia. Am. J. Trop. Med. Hyg. 12:1963;840-843
33. Singhasivanon P. Mekong Malaria. Malaria, multi-drug resistance and economic development in the greater Mekong subregion of Southeast Asia. Southeast Asian J. Trop. Med. Public Health. 30(Suppl. 4):1999;1-101
34. Ariey F., Duchemin J.B., Robert V. Metapopulation concepts applied to falciparum malaria and their impacts on the emergence and spread of chloroquine resistance. Inf. Gen. Evol. 60:2002;1-8
35. Wootton J.C., Feng X., Ferdig M.T., et al. Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum. Nature. 418:2002;320-323
36. Durand R., Jafari S., Vauzelle J., Delabre J.F., Jezic Z., Le Bras J. Analysis of pfcrt point mutation and chloroquine susceptibility in isolates of Plasmodium falciparum. Mol. Biochem. Parasitol. 114:2001;95-102
37. Thomas H., Coley H.M. Overcoming multidrug resistance in cancer: an update on the clinical strategy of inhibiting P-glycoprotein. Cancer Control. 10:2003;159-165
38. Cowman A.F., Galatis D., Thompson J.K. Selection for mefloquine resistance in Plasmodium falciparum is linked to amplification of the pfmdr1 gene and cross-resistance to halofantrine and quinine. Proc. Natl. Acad. Sci. U.S.A. 91:1994;1143-1147
39. Waller K.L., Muhle R.A., Ursos L.M., et al. Chloroquine resistance modulated in vitro by expression levels of the Plasmodium falciparum chloroquine resistance transporter (PfCRT). J. Biol Chem. 278:2003;33593-33601
40. Foote S.J., Kyle D.E., Martin R.K., et al. Severals alleles of the multidrug resistance gene are closely linked to chloroquine resistance in Plasmodium falciparum. Nature. 345:1990;255-258
41. McCutcheon K.R.G., Freese J.A., Frean J.A., Sharp B.L., Markus M.B. Two mutations in the multidrug-resistance gene homologue of Plasmodium falciparum, pfmdr1, are not useful predictors of in vivo or in vitro chloroquine resistance in southern Africa. Trans. R. Soc. Trop. Med. Hyg. 93:1999;300-302
42. Reed M.B., Saliba K.J., Caruana S.R., Kirk K., Cowman A.F. Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum. Nature. 403:2000;906-909
43. Awad-el-Kariem F.M., Miles M.A., Warhurst D.C. Chloroquine-resistant Plasmodium falciparum isolates from the Sudan lack two mutations in the pfmdr1 gene thought to be associated with chloroquine resistance. Trans. R. Soc. Trop. Med. Hyg. 86:1992;587-589
44. Basco L.K., de Pecoulas P.E., Le Bras J., Wilson C.M. Plasmodium falciparum: molecular characterisation of multidrug-resistant Cambodian isolates. Exp. Parasitol. 82:1996;97-103
45. Mu J., Ferdig M.T., Feng X., et al. Multiple transporters associated with malaria parasite responses to chloroquine and quinine. Mol. Microbiol. 49:2003;977-989