Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: Comparison of endpoint and real-time methods

Analytical Biochemistry

Volumn 285, Issue 2, 2000, Pages 194-204

  Schmittgen, Thomas D ^a   Zakrajsek, Brian A ^a   Mills, Alan G ^b   Gorn, Vladimir ^b   Singer, Michael J ^b   Reed, Michael W ^b  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b Nanogen Inc USA   (United States)

Author keywords

Endpoint PCR; Gene expression; mRNA stability; Real time PCR; SYBR green; TaqMan

Indexed keywords

CELL CULTURE; DYES; FIBROBLASTS; GENE EXPRESSION; PROBES; PROTEINS; TRANSCRIPTION;

ENDPOINT PCR; MRNA STABILITY; REAL-TIME PCR; SYBR GREEN; TAQMAN;

POLYMERASE CHAIN REACTION;

BETA GLOBIN; MESSENGER RNA; MYC PROTEIN; PROTEIN C FOS;

3' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; MOUSE; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA TRANSCRIPTION;

ANIMALIA; RODENTIA;

EID: 0034668118     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4753     Document Type: Article

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