Expression library immunization: A road map for discovery of vaccines against infectious diseases

Infection and Immunity

Volumn 73, Issue 11, 2005, Pages 7089-7098

  Talaat, Adel M ^a   Stemke Hale, Katherine ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BCG VACCINE; COMPLEMENTARY DNA; HLA ANTIGEN; IMMUNOLOGICAL ADJUVANT; PLASMID DNA; RNA; VACCINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; BACTERIAL INFECTION; BCG VACCINATION; DNA LIBRARY; DNA MICROARRAY; EXPERIMENTAL MODEL; GENE EXPRESSION; GENOME; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNIZATION; INFECTION; MOUSE; NONHUMAN; OPEN READING FRAME; PARASITOSIS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RECOMBINANT DNA TECHNOLOGY; SHORT SURVEY; TUBERCULOSIS; VIRUS INFECTION;

ANIMALS; COMMUNICABLE DISEASE CONTROL; COMPUTATIONAL BIOLOGY; GENOMIC LIBRARY; GENOMICS; INFECTION; VACCINES;

EID: 27744605276     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.73.11.7089-7098.2005     Document Type: Short Survey

References (96)

1. Alberti, E., A. Acosta, M. E. Sarmiento, C. Hidalgo, T. Vidal, A. Fachado, L. Fonte, L. Izquierdo, J. F. Infante, C. M. Finlay, and G. Sierra. 1998. Specific cellular and humoral immune response in Balb-c mice immunised with an expression genomic library of Trypanosoma cruzi. Vaccine 16:608-612.
2. Almazan, C., K. M. Kocan, D. K. Bergman, J. C. Garcia-Garcia, E. F. Blouin, and J. de la Fuente. 2003. Identification of protective antigens for the control of Ixodes scapularis infestations using cDNA expression library immunization. Vaccine 21:1492-1501.
3. Ariel, N., A. Zvi, H. Grosfeld, O. Gat, Y. Inbar, B. Velan, S. Cohen, and A. Shafferman. 2002. Search for potential vaccine candidate open reading frames in the Bacillus anthracis virulence plasmid pXO1: in silico and in vitro screening. Infect. Immun. 70:6817-6827.
4. Ash, C. 1999. Exploring parasite genomes. Trends Microbiol. 7:10-12.
5. Babiuk, L. A., S. L. Babiuk, B. I. Loehr, and S. van Drunnen Littel-van den Hurk. 2000. Nucleic acid vaccines: research tool or commercial reality. Vet. Immunol. Immunopathol. 76:1-23.
6. Babiuk, L. A., P. J. Lewis, L. van Drunen, S. Tikoo, and X. Liang. 1998. Nucleic acid vaccines: veterinary applications. Curr. Top. Microbiol. Immunol. 226:90-106.
7. Barry, M. A., D. P. Howell, H. A. Andersson, J. L. Chen, and R. A. Singh. 2004. Expression library immunization to discover and improve vaccine antigens. Immunol. Rev. 199:68-83.
8. Barry, M. A., and S. A. Johnston. 1997. Biological features of genetic immunization. Vaccine 15:788-791.
9. Barry, M. A., W. C. Lai, and S. A. Johnston. 1995. Protection against mycoplasma infection using expression-library immunization. Nature 377: 632-4535.
10. Bennett, A. M., R. J. Phillpotts, S. D. Perkins, S. C. Jacobs, and E. D. Williamson. 1999. Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan equine encephalitis virus. Vaccine 18: 588-596.
11. Bonanni, P. 1999. Demographic impact of vaccination: a review. Vaccine 17(Suppl. 3):S120-S125.
12. Bot, A., S. Antohi, S. Bot, A. Garcia-Sastre, and C. Bona. 1997. Induction of humoral and cellular immunity against influenza virus by immunization of newborn mice with a plasmid bearing a hemagglutinin gene. Int. Immunol. 9:1641-1650.
13. Brayton, K. A., M. van der Walt, S. W. Vogel, and B. A. Allsopp. 1998. A partially protective clone from Cowdria ruminantium identified by using a Salmonella vaccine delivery system. Ann. N. Y. Acad. Sci. 849:247-252.
14. Brayton, K. A., S. W. Vogel, and B. A. Allsopp. 1998. Expression library immunization to identify protective antigens from Cowdria ruminantium. Ann. N. Y. Acad. Sci. 849:369-371.
15. Buer, J., and R. Balling. 2003. Mice, microbes and models of infection. Nat. Rev. Genet. 4:195-205.
16. Cello, J., A. V. Paul, and E. Wimmer. 2002. Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template. Science 297:1016-1018.
17. Cohen, D., N. Orr, M. Haim, S. Ashkenazi, G. Robin, M. S. Green, M. Ephros, T. Sela, R. Slepon, I. Ashkenazi, D. N. Taylor, A.-M. Svennerholm, A. Eldad, and J. Shemer. 2000. Safety and immunogenicity of two different lots of the oral, killed enterotoxigenic Escherichia coli-cholera toxin B subunit vaccine in Israeli young adults. Infect. Immun. 68:4492-4497.
18. Cohn, D. L. 1997. Use of the bacille Calmette-Guerin vaccination for the prevention of tuberculosis: renewed interest in an old vaccine. Am. J. Med. Sci. 313:372-376.
19. Cole, S. T. 2002. Comparative mycobacterial genomics as a tool for drug target and antigen discovery. Eur. Respir. J. 20:78S-86S.
20. Comstock, G. W. 1994. Field trials of tuberculosis vaccines: how could we have done them better? Cont. Clin. Trials 94:247-276.
21. Couillin, I., F. Letourneur, P. Lefebvre, J. G. Guillet, and F. Martinon. 2001. DNA vaccination of macaques with several different Nef sequences induces multispecific T cell responses. Virology 279:136-145.
22. De Groot, A. S., A. Bosma, N. Chinai, J. Frost, B. M. Jesdale, M. A. Gonzalez, and W. Martin. 2001. From genome to vaccine: in silico predictions, ex vivo verification. Vaccine 19:4385-4395.
23. De Groot, A. S., and R. Rappuoli. 2004. Genome-derived vaccines. Expert Rev. Vaccines 3:59-76.
24. Delogu, G., A. Li, C. Repique, F. Collins, and S. L. Morris. 2002. DNA vaccine combinations expressing either tissue plasminogen activator signal sequence fusion proteins or ubiquitin-conjugated antigens induce sustained protective immunity in a mouse model of pulmonary tuberculosis. Infect. Immun. 70:292-302.
25. Deml, L., A. Bojak, S. Steck, M. Graf, J. Wild, R. Schirmbeck, H. Wolf, and R. Wagner. 2001. Multiple effects of codon usage optimization on expression and immunogenicity of DNA candidate vaccines encoding the human immunodeficiency virus type 1 Gag protein. J. Virol. 75:10991-11001.
26. Derrick, S. C., C. Repique, P. Snoy, A. L. Yang, and S. Morris. 2004. Immunization with a DNA vaccine cocktail protects mice lacking CD4 cells against an aerogenic infection with Mycobacterium tuberculosis. Infect. Immun. 72:1685-1692.
27. Dietrich, W. F. 2001. Using mouse genetics to understand infectious disease pathogenesis. Genome Res. 11:325-331.
28. Dillon, D. C., M. R. Alderson, C. H. Day, D. M. Lewinsohn, R. Coler, T. Bernent, A. Campos-Neto, Y. A. Skeiky, I. M. Orme, A. Roberts, S. Steen, W. Dalemans, R. Badaro, and S. G. Reed. 1999. Molecular characterization and human T-cell responses to a member of a novel Mycobacterium tuberculosis mtb39 gene family. Infect. Immun. 67:2941-2950.
29. Donnelly, J. J., A. Friedman, J. B. Ulmer, and M. A. Liu. 1997. Further protection against antigenic drift of influenza virus in a ferret model by DNA vaccination. Vaccine 15:865-868.
30. Donnelly, J. J., M. A. Liu, and J. B. Ulmer. 2000. Antigen presentation and DNA vaccines. Am. J. Respir. Crit. Care Med. 162:S190-S193.
31. Doolan, D. L., and S. L. Hoffman. 1997. Pre-erythrocytic-stage immune effector mechanisms in Plasmodium spp. infections. Philos. Trans. R. Soc. Lond. B Biol. Sci. 352:1361-1367.
32. Fachado, A., A. Rodriguez, J. Molina, J. C. Silverio, A. P. Marino, L. M. Pinto, S. O. Angel, J. F. Infante, Y. Traub-Cseko, R. R. Amendoeira, and J. Lannes-Vieira. 2003. Long-term protective immune response elicited by vaccination with an expression genomic library of Toxoplasma gondii. Infect. Immun. 71:5407-5411.
33. Fauci, A. S. 2001. Infectious diseases: considerations for the 21st century. Clin. Infect. Dis. 32:675-685.
34. Fu, T. M., L. M. Guan, A. Friedman, T. L. Schofield, J. B. Ulmer, M. A. Liu, and J. J. Donnelly. 1999. Dose dependence of CTL precursor frequency induced by a DNA vaccine and correlation with protective immunity against influenza virus challenge. J. Immunol. 162:4163-4170.
35. Griffin, J. F. T. 2002. A strategic approach to vaccine development: animal models, monitoring vaccine efficacy, formulation and delivery. Adv. Drug Deliv. Rev. 54:851-861.
36. Guan, Y., B. J. Zheng, Y. Q. He, X. L. Liu, Z. X. Zhuang, C. L. Cheung, S. W. Luo, P. H. Li, L. J. Hang, Y. J. Gan, K. M. Butt, K. L. Wong, K. W. Chan, W. Lim, K. F. Shortridge, K. Y. Yuen, J. S. Peiris, and L. L. Poon. 2002. Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China. Science 302:276-278.
37. Hansson, M., P. A. Nygren, and S. Stahl. 2000. Design and production of recombinant subunit vaccines. Biotechnol. Appl. Biochem. 32:95-107.
38. Hassani, M., M. C. Patel, and L. A. Pirofski. 2004. Vaccines for the prevention of diseases caused by potential bioweapons. Clin. Immunol. 111:1-15.
39. Hassett, D. E., J. Zhang, M. Slifka, and J. L. Whitton. 2000. Immune responses following neonatal DNA vaccination are long-lived, abundant, and qualitatively similar to those induced by conventional immunization. J. Virol. 74:2620-2627.
40. Hauser, H., and S. Y. Chen. 2003. Augmentation of DNA vaccine potency through secretory heat shock protein-mediated antigen targeting. Methods 31:225-231.
41. Hoffman, S. L., D. L. Doolan, M. Sedegah, J. C. Aguiar, R. B. Wang, A. Malik, R. A. Gramzinski, W. R. Weiss, P. Hobart, J. A. Norman, M. Margalith, and R. C. Hedstrom. 1997. Strategy for development of a pre-erythrocytic Plasmodium falciparum DNA vaccine for human use. Vaccine 15: 842-845.
42. Hsieh, M. J., A. P. Junqueira-Kipnis, A. Hoeffer, O. C. Turner, and I. M. Orme. 2004. Incorporation of CpG oligodeoxynucleotide fails to enhance the protective efficacy of a subunit vaccine against Mycobacterium tuberculosis. Vaccine 22:655-659.
43. Ivey, F. D., D. M. Magee, M. D. Woitaske, S. A. Johnston, and R. A. Cox. 2003. Identification of a protective antigen of Coccidioides immitis by expression library immunization. Vaccine 21:4359-4367.
44. Kalinna, B. H. 1997. DNA vaccines for parasitic infections. Immunol. Cell Biol. 75:370-375.
45. Kim, J. J., J. S. Yang, L. K. Nottingham, D. J. Lee, M. Lee, K. H. Manson, M. S. Wyand, J. D. Boyer, K. E. Ugen, and D. B. Weiner. 2001. Protection from immunodeficiency virus challenges in rhesus macaques by multicomponent DNA immunization. Virology 285:204-217.
46. Kindhauser, M. K. 2003. Global defence against the infectious disease threat, p. 1-231. In World Health Organization Report Communicable Diseases 2002, vol. 15. World Health Organization, Geneva, Switzerland.
47. Lazowska, I., L. Trzeciak, R. Godlewska, E. Hennig, K. Jagusztyn-Krynicka, J. Popowski, J. Regula, and J. Ostrowski. 2000. In search of immunogenic Helicobacter pylori proteins by screening of expression library. Digestion 61:14-21.
48. Leclercq, S. Y., and S. C. Oliveira. 2003. Protective immunity induced by DNA-library immunization against an intracellular bacterial infection. J. Drug Target. 11:531-538.
49. Levine, M. M., J. B. Kaper, D. Hermann, J. Ketley, G. Losonsky, C. O. Tacket, B. Tall, and S. Cryz. 1988. Safety, immunogenicity, and efficacy of recombinant live oral cholera vaccines, CVD 103 and CVD 103-HgR. Lancet ii:467-470.
50. Li, Z., A. Howard, C. Kelley, G. Delogu, F. Collins, and S. Morris. 1999. Immunogenicity of DNA vaccines expressing tuberculosis proteins fused to tissue plasminogen activator signal sequences. Infect. Immun. 67:4780-4786.
51. Liu, M. A., W. McClements, J. B. Ulmer, J. Shiver, and J. Donnelly. 1997. Immunization of non-human primates with DNA vaccines. Vaccine 15:909-912.
52. Locher, C. P., K. F. Sykes, D. J. Blackbourn, and S. A. Johnston. 2002. Immune responses in baboons vaccinated with HIV-2 genetic expression libraries. J. Med. Primatol. 31:323-329.
53. Lowrie, D. B., R. E. Tascon, V. L. Bonato, V. M. Lima, L. H. Faccioli, E. Stavropoulos, M. J. Colston, et al. 1999. Therapy of tuberculosis in mice by DNA vaccination. Nature 400:269-271.
54. Lunn, D. P., G. Soboll, B. R. Schram, J. Quass, M. W. McGregor, R. J. Drape, M. D. Macklin, D. E. McCabe, W. F. Swain, and C. W. Olsen. 1999. Antibody responses to DNA vaccination of horses using the influenza virus hemagglutinin gene. Vaccine 17:2245-2258.
55. Manoutcharian, K., L. I. Terrazas, G. Gevorkian, and T. Govezensky. 1998. Protection against murine cysticercosis using cDNA expression library immunization. Immunol. Lett. 62:131-136.
56. Martin, E., A. T. Kamath, H. Briscoe, and W. J. Britton. 2003. The combination of plasmid interleukin-12 with a single DNA vaccine is more effective than Mycobacterium bovis (bacille Calmette-Guerin) in protecting against systemic Mycobacterium avium infection. Immunology 109:308-314.
57. Melby, P. C., N. Biediger, H. Flores, C. Geldmacher, M. Melendez, G. B. Ogden, J. Uranga, and W. Zhao. 1999. Identification of vaccine candidates against visceral leishmaniasis by expression library immunization. Am. J. Trop. Med. Hyg. 61:379.
58. Melby, P. C., G. B. Ogden, H. Flores, W. Zhao, C. Gelmacher, J. Uranga, N. M. Biediger, S. K. Ahuja, J. Uranga, and M. Melendez. 2000. Identification of vaccine candidates for experimental visceral leishmaniasis by immunization with sequential fractions of a cDNA expression library. Infect. Immun. 68:5595-5602.
59. Merrell, D. S., S. M. Butler, F. Qadri, N. A. Dolganov, A. Alam, M. B. Cohen, S. B. Calderwood, G. K. Schoolnik, and A. Camilli. 2002. Host-induced epidemic spread of the cholera bacterium. Nature 417:642-645.
60. Miquel, A., I. Muller, P. Ferrer, P. D. Valenzuela, and L. O. Burzio. 2003. Immunoresponse of Coho salmon immunized with a gene expression library from Piscirickettsia salmonis. Biol. Res. 36:313-323.
61. Moore, R. J., C. Lenghaus, S. A. Sheedy, and T. J. Doran. 2001. Improved vectors for expression library immunization-application to Mycoplasma hyopneumoniae infection in pigs. Vaccine 20:115-120.
62. Mora, M., D. Veggi, L. Santini, M. Pizza, and R. Rappuoli. 2003. Reverse vaccinology. Drug Discov. Today 8:459-464.
63. Morris, S., C. Kelley, A. Howard, Z. M. Li, and F. Collins. 2000. The immunogenicity of single and combination DNA vaccines against tuberculosis. Vaccine 18:2155-2163.
64. Nagata, T., M. Uchijima, A. Yoshida, M. Kawashima, and Y. Koide. 1999. Codon optimization effect on translational efficiency of DNA vaccine in mammalian cells: analysis of plasmid DNA encoding a CTL epitope derived from microorganisms. Biochem. Biophys. Res. Commun. 261:445-451.
65. Orihuela, C. J., J. N. Radin, J. E. Sublett, G. L. Gao, D. Kaushal, and E. I. Tuomanen. 2004. Microarray analysis of pneumococcal gene expression during invasive disease. Infect. Immun. 72:5582-5596.
66. Piedrafita, D., D. M. Xu, D. Hunter, R. A. Harrison, and F. Y. Liew. 1999. Protective immune responses induced by vaccination with an expression genomic library of Leishmania major. J. Immunol. 163:1467-1472.
67. Rainczuk, A., T. Scorza, P. M. Smooker, and T. W. Spithill. 2003. Induction of specific T-cell responses, opsonizing antibodies, and protection against Plasmodium chabaudi adami infection in mice vaccinated with genomic expression libraries expressed in targeted and secretory DNA vectors. Infect. Immun. 71:4506-4515.
68. Revel, A. T., A. M. Talaat, and M. V. Norgard. 2002. DNA microarray analysis of differential gene expression in Boirelia burgdorferi, the Lyme disease spirochete. Proc. Natl. Acad. Sci. USA 99:1562-1567.
69. Robinson, H. L., C. A. Boyle, D. M. Feltquate, M. J. Morin, J. C. Santoro, and R. G. Webster. 1997. DNA immunization for influenza virus: studies using hemagglutinin- and nucleoprotein-expressing DNAs. J. Infect. Dis. 176(Suppl. 1):S50-S55.
70. Rombel, I. T., K. F. Sykes, S. Rayner, and S. A. Johnston. 2002. ORF-FINDER: a vector for high-throughput gene identification. Gene 282:33-41.
71. Rosenthal, S. R., M. Merchlinsky, C. Kleppinger, and K. L. Goldenthal. 2001. Developing new smallpox vaccines. Emerg. Infect. Dis. 7:920-926.
72. Sasaki, S., T. Tsuji, Y. Asakura, J. Fukushima, and K. Okuda. 1998. The search for a potent DNA vaccine against AIDS: the enhancement of immunogenicity by chemical and genetic adjuvants. Anticancer Res. 18:3907-3915.
73. Singh, R. A., and M. A. Barry. 2004. Repertoire and immunofocusing of CD8 T cell responses generated by HIV-1 gag-pol and expression library immunization vaccines. J. Immunol. 173:4387-4393.
74. Singh, R. A., L. Wu, and M. A. Barry. 2002. Generation of genome-wide CD8 T cell responses in HLA-A*0201 transgenic mice by an HIV-1 ubiquitin expression library immunization vaccine. J. Immunol. 168:379-391.
75. Skeiky, Y. A., P. J. Ovendale, S. Jen, M. R. Alderson, D. C. Dillon, S. Smith, C. B. Wilson, I. M. Orme, S. G. Reed, and A. Campos-Neto. 2000. T cell expression cloning of a Mycobacterium tuberculosis gene encoding a protective antigen associated with the early control of infection. J. Immunol. 165: 7140-7149.
76. Smith, C., P. J. R. Day, and M. R. Walker. 1993. Generation of cohesive ends on PCR products by UDG-mediated excision of dU, and application for cloning into restriction digest-linearized vectors. PCR Methods Appl. 2:328-332.
77. Smooker, P. M., Y. Y. Setiady, A. Rainczuk, and T. W. Spithill. 2000. Expression library immunization protects mice against a challenge with virulent rodent malaria. Vaccine 18:2533-2540.
78. Snyder, J. A., B. J. Haugen, E. L. Buckles, C. V. Lockatell, D. E. Johnson, M. S. Donnenberg, R. A. Welch, and H. L. T. Mobley. 2004. Transcriptome of uropathogenic Escherichia coli during urinary tract infection. Infect. Immun. 72:6373-6381.
79. Stemke-Hale, K., B. Kaltenboeck, F. J. DeGraves, K. F. Sykes, J. Huang, C.-H. Bu, and S. A. Johnston. 2005. Screening the whole genome of a pathogen in vivo for individual protective antigens. Vaccine 23:3016-3025.
80. Stephen, J. A., A. M. Talaat, and M. J. McGuire. 2002. Genetic immunization: what's in a name? Arch. Med. Res. 33:325-329.
81. Sturniolo, T., E. Bono, J. Y. Ding, L. Raddrizzani, O. Tuereci, U. Sahin, M. Braxenthaler, F. Gallazzi, M. P. Protti, F. Sinigaglia, and J. Hammer. 1999. Generation of tissue-specific and promiscuous HLA ligand databases using DNA microarrays and virtual HLA class II matrices. Nat. Biotechnol. 17: 555-561.
82. Suarez, D. L., M. L. Perdue, N. Cox, T. Rowe, C. Bender, J. Huang, and D. E. Swayne. 1998. Comparisons of highly virulent H5N1 influenza A viruses isolated from humans and chickens from Hong Kong. J. Virol. 72:6678-6688.
83. Sykes, K. F., and S. A. Johnston. 1999. Genetic live vaccines mimic the antigenicity but not pathogenicity of live viruses. DNA Cell Biol. 18:521-531.
84. Sykes, K. F., and S. A. Johnston. 1999. Linear expression elements: a rapid, in vivo, method to screen for gene functions. Nat. Biotechnol. 17:355-359.
85. Sykes, K. F., M. G. Lewis, B. Squires, and S. A. Johnston. 2002. Evaluation of SIV library vaccines with genetic cytokines in a macaque challenge. Vaccine 20:2382-2395.
86. Tacket, C. O., M. B. Sztein, G. A. Losonsky, S. S. Wasserman, J. P. Nataro, R. Edelman, D. Pickard, G. Dougan, S. N. Chatfield, and M. M. Levine. 1997. Safety of live oral Salmonella typhi vaccine strains with deletions in htrA and aroC aroD and immune response in humans. Infect. Immun. 65:452-456.
87. Talaat, A. M., P. Hunter, and S. A. Johnston. 2000. Genome-directed primers for selective labeling of bacterial transcripts for DNA microarray analysis. Nat. Biotechnol. 18:679-682.
88. Talaat, A. M., R. Lyons, S. T. Howard, and S. A. Johnston. 2004. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Proc. Natl. Acad. Sci. USA 101:4602-4607.
89. Talaat, A. M., R. Lyons, and S. A. Johnston. 2001. A combination vaccine confers full protection against co-infections with influenza, herpes simplex and respiratory syncytial viruses. Vaccine 20:538-544.
90. Ulmer, J. B., R. R. Deck, C. M. DeWitt, T. M. Fu, J. J. Donnelly, M. J. Caulfield, and M. A. Liu. 1997. Expression of a viral protein by muscle cells in vivo induces protective cell-mediated immunity. Vaccine 15:839-841.
91. United States National Intelligence Council. 2000. The global infectious disease threat and its implications for the United States. Environ. Change Secur. Proj. Rep. Summer:33-65.
92. van den Hurk, S. V., V. Gerdts, B. I. Loehr, R. Pontarollo, R. Rankin, R. Uwiera, and L. A. Babiuk. 2000. Recent advances in the use of DNA vaccines for the treatment of diseases of farmed animals. Adv. Drug Deliv. Rev. 43:13-28.
93. Wang, Q. M., S. H. Sun, Z. L. Hu, M. Yin, C. J. Xiao, and J. C. Zhang. 2004. Improved immunogenicity of a tuberculosis DNA vaccine encoding ESAT6 by DNA priming and protein boosting. Vaccine 22:3622-3627.
94. Wang, R. B., D. L. Doolan, T. P. Le, R. C. Hedstrom, K. M. Coonan, Y. P. Charoenvit, T. R. Jones, P. Hobart, et al. 1998. Induction of antigen-specific cytotoxic T lymphocytes in humans by a malaria DNA vaccine. Science 282:476-480.
95. Yero, C. D., F. R. Pajon, M. E. Caballero, A. K. Cobas, H. Y. Lopez, M. M. Farinas, B. S. Gonzales, and D. A. Acosta. 2005. Immunization of mice with Neisseria meningitidis serogroup B genomic expression libraries elicits functional antibodies and reduces the level of bacteremia in an infant rat infection model. Vaccine 23:932-939.
96. Zagursky, R. J., and D. Russell. 2001. Bioinformatics: use in bacterial vaccine discovery. BioTechniques 31:636-640.