Isolation and characterization of viruses related to the SARS coronavirus from animals in Southern China

Science

Volumn 302, Issue 5643, 2003, Pages 276-278

  Guan, Y ^a   Zheng, B J ^a   He, Y Q ^b   Liu, X L ^b   Zhuang, Z X ^b   Cheung, C L ^a   Luo, S W ^a   Li, P H ^a   Zhang, L J ^a   Guan, Y J ^a   Butt, K M ^a   Wong, K L ^a   Chan, K W ^a   Lim, W ^c   Shortridge, K F ^a   Yuen, K Y ^a   Peiris, J S M ^a   Poon, L L M ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b SHENZHEN CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

^c DEPARTMENT OF HEALTH   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

DISEASE CONTROL; GENES;

NUCLEOTIDES;

VIRUSES;

ANIMAL; SEVERE ACUTE RESPIRATORY SYNDROME; VIRUS;

ANIMAL; ARTICLE; CHINA; CONTROLLED STUDY; CORONAVIRUS; HIMALAYAN PALM CIVET; HUMAN; MARKET; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RACCOON DOG; RESPIRATORY TRACT DISEASE; SARS CORONAVIRUS; SEVERE ACUTE RESPIRATORY SYNDROME; VIRUS CHARACTERIZATION; VIRUS INFECTION; VIRUS ISOLATION; VIRUS TRANSMISSION;

AMINO ACID SEQUENCE; ANIMALS; ANIMALS, WILD; ANTIBODIES, VIRAL; BLOTTING, WESTERN; CARNIVORA; CHINA; CORONAVIRUS; CORONAVIRUS INFECTIONS; DISEASE RESERVOIRS; FECES; GENOME, VIRAL; HUMANS; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; NEUTRALIZATION TESTS; NOSE; OPEN READING FRAMES; PHYLOGENY; POLYMORPHISM, GENETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SARS VIRUS; SEQUENCE DELETION; SEQUENCE HOMOLOGY, NUCLEIC ACID; VIRAL ENVELOPE PROTEINS; VIRAL PROTEINS;

CHINA;

ANIMALIA; CANIS FAMILIARIS; CORONAVIRUS; MAMMALIA; NYCTEREUTES PROCYONOIDES; PROCYON LOTOR; RNA VIRUSES; SARS CORONAVIRUS; VIVERRIDAE;

EID: 0141889936     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1087139     Document Type: Article

References (12)

1. World Health Organization (WHO) (www.who.int/csr/sars/enl).
2. WHO, Cumulative Number of Reported Probable Cases of Severe Acute Respiratory Syndrome (SARS) (www. who.int/csr/sars/country/2003_05_20/en/).
3. J. S. M. Peiris et al., Lancet 361, 1319 (2003).
4. T. G. Ksiazek et al., N. Engl. J. Med. 348, 1953 (2003).
5. R. A. Fouchier et al., Nature 423, 240 (2003).
6. N. S. Zhong et al., Lancet, in press.
7. K. Holmes, unpublished observations.
8. M. A. Marra et al., Science 300, 1399 (2003).
9. China species information system (www. chinabiodiversity.com).
10. S. Kumar et al., Bioinformatics 17, 1244 (2001).
11. M. Kimura, J. Mol. Evol. 16, 111 (1980).
12. We thank the Department of Health and Department of Agriculture of Shenzhen Government for facilitating the study. We gratefully acknowledge the encouragement and support of L. C. Tsui, Vice-Chancellor, The University of Hong Kong. We thank X. Y. Zhao from the Department of the Microbiology, The University of Hong Kong, for the excellent technical assistance. We also thank C. C. Hon and F. C. Leung from the Department of Zoology, The University of Hong Kong, and Richard Webby from St. Jude Children's Research Hospital (Memphis, TN, U.S.A.) for assistance in the phylogenetic analysis. We thank K. V. Holmes's laboratory from the Department of Microbiology, University of Colorado Health Sciences Center (Denver, CO, U.S.A.) to validate the animal viral sequences. Supported by research funding from Public Health Research (Grant A195357), the U.S. National Institute of Allergy and Infectious Diseases, the Wellcome Trust (067072/D/02/Z), and SARS research funds from The University of Hong Kong.