Structure and hydride transfer mechanism of a moderate thermophilic dihydrofolate reductase from Bacillus stearothermophilus and comparison to its mesophilic and hyperthermophilic homologues

Biochemistry

Volumn 44, Issue 34, 2005, Pages 11428-11439

  Kim, Hui Sun ^a   Damo, Steven M ^a   Lee, Seok Yong ^a   Wemmer, David ^a   Klinman, Judith P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOMES; CLONING; COMPLEXATION; HYDRIDES; ISOTOPES; THERMAL EFFECTS; X RAY ANALYSIS;

ELEVATED TEMPERATURES; KINETIC MECHANISMS; MOLECULAR MASS; SECOND-ORDER REACTION;

ENZYMES;

DIHYDROFOLATE REDUCTASE; DIHYDROFOLIC ACID; HYDROGEN; ISOTOPE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ALKALINITY; AMINO ACID SEQUENCE; ARTICLE; CALCULATION; CHROMOSOME; COMPLEX FORMATION; ENTHALPY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; GEOBACILLUS STEAROTHERMOPHILUS; HIGH TEMPERATURE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REACTION ANALYSIS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; STEADY STATE; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; THERMOPHILIC BACTERIUM; X RAY ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; BACILLUS STEAROTHERMOPHILUS; CLONING, MOLECULAR; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; HUMANS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY; TETRAHYDROFOLATE DEHYDROGENASE;

ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; LACTOBACILLUS CASEI; THERMOTOGA MARITIMA;

EID: 23944518876     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi050630j     Document Type: Article

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