Expression and induction potential of cytochromes P450 in human cryopreserved hepatocytes

Drug Metabolism and Disposition

Volumn 33, Issue 7, 2005, Pages 1004-1016

  Roymans, Dirk ^a,c   Annaert, Pieter ^a   Van Houdt, Jos ^a   Weygers, Adri ^a   Noukens, Jan ^a   Sensenhauser, Carlo ^b   Silva, José ^b   Van Looveren, Cis ^a   Hendrickx, Jan ^a   Mannens, Geert ^a   Meuldermans, Willem ^a  

^a JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^b JOHNSON AND JOHNSON PHARMACEUTICAL RESEARCH AND DEVELOPMENT   (United States)

^c Lead Discovery Operations   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; CYTOCHROME P450 1A2; CYTOCHROME P450 2B6; CYTOCHROME P450 2C9; CYTOCHROME P450 2E1; MESSENGER RNA;

ARTICLE; CELL CULTURE; CELL VIABILITY; CRYOPRESERVATION; DONOR; ENZYME ASSAY; ENZYME INDUCTION; HUMAN; HUMAN TISSUE; INCUBATION TIME; LIVER CELL; MEASUREMENT; MONITORING; MORPHOLOGY; NUCLEOTIDE SEQUENCE; PLATING MEDIUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; THAWING;

BASE SEQUENCE; BLOTTING, WESTERN; CELLS, CULTURED; CRYOPRESERVATION; CYTOCHROME P-450 ENZYME SYSTEM; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION PROFILING; HEPATOCYTES; HUMANS;

EID: 22344448841     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: 10.1124/dmd.104.003046     Document Type: Article

References (49)

1. Alexandre E, Viollon-Abadie C, David P, Gandillet A, Coassolo P, Heyd B, Mantion G, Wolf P, Bachellier P, Jaeck D, et al. (2002) Cryopreservation of adult human hepatocytes obtained from resected liver biopsies. Cryobiology 44:103-113.
2. Bjornsson TD, Callaghan JT, Einolf HJ, Fischer V, Gan L, Grimm S, Kao J, King SP, Miwa G, Ni L, et al. (2003) The conduct of in vitro and in vivo drug-drug interaction studies: a Pharmaceutical Research and Manufacturers of America (PhRMA) perspective. Drug Metab Dispos 31:815-832.
3. Carpenter SP, Lasker JM, and Raucy JL (1996) Expression, induction and catalytic activity of the ethanol-inducible cytochrome P450 (CYP2E1) in human fetal liver and hepatocytes. Mol Pharmacol 49:260-268.
4. Chang TK, Yu L, Maurel P, and Waxman DJ (1997) Enhanced cyclophosphamide and ifosfamide activation in primary human hepatocyte cultures: response to cytochrome P-450 inducers and autoinduction by oxazaphosphorines. Cancer Res 57:1946-1954.
5. Cohen LH, van Leeuwen REW, van Thiel GCF, van Pelt JF, and Yap SH (2000) Equally potent inhibitors of cholesterol synthesis in human hepatocytes have distinguishable effects on different cytochrome P450 enzymes. Biopharm Drug Dispos 21:353-364.
6. Curi-Pedrosa R, Daujat M, Pichard L, Ourlin JC, Clair P, Gervot L, Lesca P, Domergue J, Joyeux H, Fourtanier G, et al. (1994) Omeprazole and lansoprazole are mixed inducers of CYP1A and CYP3A in human hepatocytes in primary culture. J Pharmacol Exp Ther 269:384-392.
7. Dilger K, Zheng Z, and Klotz U (1999) Lack of drug interaction between omeprazole, lansoprazole, pantoprazole and theophylline. Br J Clin Pharmacol 48:438-444.
8. Donato MT, Castell JV, and Gomez-Lechon MJ (1995) Effect of model inducers on cytochrome P450 activities of human hepatocytes in primary culture. Drug Metab Dispos 23:553-558.
9. Donato MT, Gómez-Lechón MJ, Jover R, Nakamura T, and Castell JV (1998) Human hepatocyte growth factor down-regulates the expression of cytochrome P450 isozymes in human hepatocytes in primary culture. J Pharmacol Exp Ther 284:760-767.
10. Edwards RJ, Price RJ, Watts PS, Renwick AB, Tredger M, Boobis AR, and Lake BG (2003) Induction of cytochrome P450 enzymes in cultured precision-cut human liver slices. Drug Metab Dispos 31:282-288.
11. El-Sankary W, Bombail V, Gibson GG, and Plant N (2002) Glucocorticoid-mediated induction of CYP3A4 is decreased by disruption of a protein:DNA interaction distinct from the pregnane X receptor response element. Drug Metab Dispos 30:1029-1034.
12. Faucette SR, Wang H, Hamilton GA, Jolley SL, Gilbert D, Lindley C, Yan B, Negishi M, and LeCluyse EL (2004) Regulation of CYP2B6 in primary human hepatocytes by prototypical inducers. Drug Metab Dispos 32:348-358.
13. Ferrini JB, Pichard L, Domergue J, and Maurel P (1997) Long-term primary cultures of adult human hepatocytes. Chem-Biol Interact 107:31-45.
14. Fuhr U (2000) Induction of drug metabolising enzymes, pharmacokinetic and toxicological consequences in humans. Clin Pharmacokinet 38:493-504.
15. Gerbal-Chaloin S, Pascussi J-M, Pichard-Garcia L, Daujat M, Waechter F, Fabre J-M, Carrère N, and Maurel P (2001) Induction of CYP2C genes in human hepatocytes in primary culture. Drug Metab Dispos 29:242-251.
16. Gervot L, Rochat B, Gautier JC, Bohnenstengel F, Kroemer H, de Berardinis V, Martin H, Beaune P, and de Waziers I (1999) Human CYP2B6: expression, inducibility and catalytic activities. Pharmacogenetics 9:295-306.
17. Guillén MI, Donato MT, Jover R, Castell JV, Fabra R, Trullenque R, and Gómez-Lechón MJ (1998) Oncostatin M down-regulates basal and induced cytochromes P450 in human hepatocytes. J Pharmacol Exp Ther 285:127-134.
18. Guillouzo A, Morel F, Langouet S, Maheo K, and Rissel M (1997) Use of hepatocyte cultures for the study of hepatotoxic compounds. J Hepatol 26:73-80.
19. Hengstler JG, Ringel M, Biefang K, Hammel S, Milbert U, Gerl M, Klebach M, Diener B, Platt KL, Böttger T, Steinberg P, and Oesch F (2000a) Cultures with cryopreserved hepatocytes: applicability for studies of enzyme induction. Chem-Biol Interact 125:51-73.
20. Hengstler JG, Utesch D, Steinberg P, Platt KL, Diener B, Ringel M, Swales N, Fischer T, Biefang K, Gerl M, et al. (2000b) Cryopreserved primary hepatocytes as a constantly available in vitro model for the evaluation of human and animal drug metabolism and enzyme induction. Drug Metab Rev 32:81-118.
21. Jang GR and Maurel PJP (2000) Drugs as inducers of metabolic enzymes: rifampicin, dexamethasone and omeprazole, in Metabolic Drug Interactions (Levy RH, Thummel KE, Trager WF, Hansten PD, and Eichelbaum M eds) pp 690-706, Lippincott Williams & Wilkins, Philadelphia.
22. Kern A, Bader A, Pichlmayr R, and Sewing KF (1997) Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochem Pharmacol 54:761-772.
23. Koop DR and Tierney DJ (1990) Multiple mechanism in the regulation of ethanol-inducible cytochrome P450IIE1. Bioessays 12:429-435.
24. Kostrubsky VE, Ramachandran V, Venkataramanan R, Dorko K, Esplen JE, Zhang S, Sinclair JF, Wrighton SA, and Strom SC (1999) The use of human hepatocyte cultures to study the induction of cytochrome P-450. Drug Metab Dispos 27:887-894.
25. Kostrubsky VE, Strom SC, Wood SG, Wrighton SA, Sinclair PR, and Sinclair JF (1995) Ethanol and isopentanol increase CYP3A and CYP2E in primary cultures of human hepatocytes. Arch Biochem Biophys 322:516-520.
26. LeCluyse EL (2001) Human hepatocyte culture systems for the in vitro evaluation of cytochrome P450 expression and regulation. Eur J Pharm Sci 13:343-368.
27. LeCluyse EL, Madan A, Hamilton G, Carroll K, DeHaan R, and Parkinson A (2000) Expression and regulation of cytochrome P450 enzymes in primary cultures of human hepatocytes. J Biochem Mol Toxicol 14:121-159.
28. Li AP, Gorycki PD, Hengstler JG, Kedderis GL, Koebe HG, Rahmani R, de Sousas G, Silva JM, and Skett P (1999) Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel. Chem-Biol Interact 121:117-123.
29. Madan A, Graham AR, Carroll KM, Mudra DR, Alayne Burton L, Krueger LA, Downey AD, Czerwinski M, Forster J, Ribadeneira MD, et al. (2003) Effects of prototypical microsomal enzyme inducers on cytochrome P450 expression in cultured human hepatocytes. Drug Metab Dispos 31:421-431.
30. Maurel P (1996) The use of adult human hepatocytes in primary culture and other in vitro systems to investigate drug metabolism in man. Adv Drug Deliv Rev 22:105-132.
31. Meunier V, Bourrié M, Marti JE, Guillou F, Berger Y, and Fabre G (2000) Expression and induction of CYP1A1/1A2, CYP2A6 and CYP3A4 in primary cultures of human hepatocytes: a 10-year follow-up. Xenobiotica 30:589-607.
32. Morel F, Beaune PH, Ratanasavanh D, Flinois JP, Yang CS, Guengerich FP, and Guillouzo A (1990) Expression of cytochrome P-450 enzymes in cultured human hepatocytes. Eur J Biochem 191:437-444.
33. Niemela O, Parkkila S, Juvonen RO, Viitala K, Gelboin HV, and PasanenM(2000) Cytochromes P450 2A6, 2E1 and 3A and production of protein-aldehyde adducts in the liver of patients with alcoholic and non-alcoholic liver diseases. J Hepatol 33:893-901.
34. Novak RF and Woodcroft KJ (2000) The alcohol-inducible form of cytochrome P450 (CYP 2E1): role in toxicology and regulation of expression. Arch Pharm Res (Seoul) 23:267-282.
35. Ono S, Hatanake T, Hotta H, Tsutsui M, Satoh T, and Gonzales FJ (1995) Chlorzoxazone is metabolised by human CYP1A2 as well as by human CYP2E1. Pharmacogenetics 5:143-150.
36. Parkinson A (2001) Biotransformation of xenobiotics, in Toxicology, the Basic Science of Poisons (Klaassen CD ed) pp 133-224, McGraw-Hill, New York.
37. Pascussi J-M, Gerbal-Chaloin S, Fabre J-M, Maurel P, and Vilarem M-J (2000) Dexamethasone enhances constitutive androstane receptor expression in human hepatocytes: consequences on cytochrome P450 gene regulation. Mol Pharmacol 58:1441-1450.
38. Rae JM, Johnson MD, Lippman ME, and Flockhart DA (2001) Rifampin is a selective, pleiotropic inducer of drug metabolism genes in human hepatocytes: studies with cDNA and oligonucleotide expression arrays. J Pharmacol Exp Ther 299:849-857.
39. Raucy JL, Mueller L, Duan K, Allen SW, Strom S, and Lasker JM (2002) Expression and induction of CYP2C P450 enzymes in primary cultures of human hepatocytes. J Pharmacol Exp Ther 302:475-482.
40. Reinach B, de Sousa G, Dostert P, Ings R, Gugenheim J, and Rahmani R (1999) Comparative effects of rifabutin and rifampicin on cytochromes P450 and UDP-glucuronosyl-transferases expression in fresh and cryopreserved human hepatocytes. Chem-Biol Interact 121:37-48.
41. Roymans D, Van Looveren C, Leone A, Parker JB, McMillian M, Johnson MD, Koganti A, Silber P, Mannens G, and Meuldermans W (2004) Determination of cytochrome P450 1A2 and cytochrome P450 3A4 induction in cryopreserved human hepatocytes. Biochem Pharmacol 67:427-437.
42. Runge D, Kohler C, Kostrubsky VE, Jager D, Lehmann T, Runge DM, May U, Stolz DB, Strom SC, Fleig WE, et al. (2000) Induction of cytochrome P450 (CYP)1A1, CYP1A2 and CYP3A4 but not of CYP2C9, CYP2C19, multidrug resistance (MDR-1) and multidrug resistance associated protein (MRP-1) by prototypical inducers in human hepatocytes. Biochem Biophys Res Commun 273:333-341.
43. Silva JM, Day SH, and Nicoll-Griffith DA (1999) Induction of cytochrome-P450 in cryopreserved rat and human hepatocytes. Chem-Biol Interact 121:49-63.
44. Silva JM, Morin PE, Day SH, Kennedy BP, Payette P, Rushmore T, Yergey JA, and Nicoll-Griffith D (1998) Refinement of an in vitro cell model for cytochrome P450 induction. Drug Metab Dispos 26:490-496.
45. Silva JM and Nicoll-Griffith DA (2002) In vitro models for studying induction of cytochrome P450 enzymes, in Drug-Drug Interactions, Drugs and the Pharmaceutical Sciences (Rodrigues AD ed) pp 189-217, Marcel Dekker, New York.
46. Skett P, Roberts P, and Khan S (1999) Maintenance of steroid metabolism and hormone responsiveness in cryopreserved dog, monkey and human hepatocytes. Chem-Biol Interact 121:65-76.
47. Skett P, Tyson C, Guillouzo A, and Maier P (1995) Report on the international workshop on the use of human liver preparations to study drug metabolism in drug development. Biochem Pharmacol 50:280-285.
48. Wang H, Faucette SR, Gilbert D, Jolley SL, Sueyoshi T, Negishi M, and LeCluyse EL (2003) Glucocorticoid receptor enhancement of pregnane X receptor-mediated CYP2B6 regulation in primary human hepatocytes. Drug Metab Dispos 31:620-630.
49. Worboys PD and Carlile DJ (2000) Implications and consequences of enzyme induction on preclinical and clinical drug development. Xenobiotica 31:539-556.