Ile115Leu mutation in the SRSI region of an insect cytochrome P450 (CYP6B1) compromises substrate turnover via changes in a predicted product release channel

Protein Engineering, Design and Selection

Volumn 18, Issue 4, 2005, Pages 191-199

  Wen, Zhimou ^a   Baudry, Jerome ^a   Berenbaum, May R ^a   Schuler, Mary A ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Cytochrome P450 monooxygenases; Insect metabolism of toxins; Molecular modeling; Site directed mutagenesis; Substrate recognition sites

Indexed keywords

AMINO ACIDS; CATALYSIS; ELECTRON TRANSITIONS; METABOLISM; PLANTS (BOTANY); TOXIC MATERIALS;

CYTOCHROME; FURANOCOUMARINS; MUTATION; TOXINS;

ENZYMES;

CYTOCHROME P450; CYTOCHROME P450 6B1; CYTOCHROME P450 REDUCTASE; FUROCOUMARIN DERIVATIVE; HEME; ISOLEUCINE; LEUCINE; METHOXSALEN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; CYTOCHROME P 450 CYP6B1 (BUTTERFLY); CYTOCHROME P-450 CYP6B1 (BUTTERFLY); PSORALEN DERIVATIVE; UNSPECIFIC MONOOXYGENASE;

ARTICLE; BINDING AFFINITY; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GENE MUTATION; INSECT; METABOLISM; MOLECULAR MODEL; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; BUTTERFLY; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; MOLECULAR GENETICS; POINT MUTATION;

INSECTA; PAPILIO POLYXENES;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ARYL HYDROCARBON HYDROXYLASES; BUTTERFLIES; CATALYTIC DOMAIN; ELECTRON TRANSPORT; ISOLEUCINE; LEUCINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; POINT MUTATION; PSORALENS; SUBSTRATE SPECIFICITY;

EID: 18844458856     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzi023     Document Type: Article

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