Optimization of an siRNA-expression system with an improved hairpin and its significant suppressive effects in mammalian cells

Journal of Gene Medicine

Volumn 6, Issue 7, 2004, Pages 715-723

  Miyagishi, Makoto ^a,b   Sumimoto, Hidetoshi ^c   Miyoshi, Hiroyuki ^d   Kawakami, Yutaka ^c   Taira, Kazunari ^a,b  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^d RIKEN TSUKUBA INSTITUTE   (Japan)

Author keywords

Gene silencing; HIV vector; RNA interference; Tetracycline regulated promoter; U6 promoter

Indexed keywords

DNA FRAGMENT; MICRORNA; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; VIRUS VECTOR;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; GENE INSERTION; GENE MUTATION; GENE SILENCING; GENETIC CODE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; MOLECULAR CLONING; POINT MUTATION; PRIORITY JOURNAL; REPORTER GENE; RNA INTERFERENCE; TRANSCRIPTION REGULATION;

BASE SEQUENCE; DNA PRIMERS; GENE SILENCING; GENES, REPORTER; GENETIC VECTORS; HELA CELLS; HIV; HUMANS; NUCLEIC ACID CONFORMATION; PROMOTER REGIONS (GENETICS); RNA, SMALL INTERFERING; TETRACYCLINE;

MAMMALIA;

EID: 13644269779     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.556     Document Type: Article

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