siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells

Nucleic Acids Research

Volumn 31, Issue 3, 2003, Pages 981-987

  Kawasaki, Hiroaki ^a,b   Suyama, Eigo ^a,b   Iyo, Mayu ^a   Taira, Kazunari ^a,b  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DICER; MESSENGER RNA; PROTEIN C FOS; PROTEIN C JUN; PUROMYCIN; RECOMBINANT PROTEIN; RNA; UNCLASSIFIED DRUG;

CONTROLLED STUDY; DNA RECOMBINATION; GENE EXPRESSION; GENE INACTIVATION; GENE SILENCING; GENE TARGETING; GENETIC RESISTANCE; HUMAN; HUMAN CELL; IN VITRO STUDY; MAMMAL CELL; ONCOGENE H RAS; PRIORITY JOURNAL; PROTEIN FAMILY; REVIEW; RNA DEGRADATION; RNA STRUCTURE; SEQUENCE HOMOLOGY;

CLONING, MOLECULAR; DRUG RESISTANCE; ENDORIBONUCLEASES; GENE TARGETING; HELA CELLS; HUMANS; PROTO-ONCOGENE PROTEINS C-FOS; PROTO-ONCOGENE PROTEINS C-JUN; PROTO-ONCOGENE PROTEINS P21(RAS); PUROMYCIN; RECOMBINANT PROTEINS; RIBONUCLEASE III; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING;

ANIMALIA; EUKARYOTA; MAMMALIA;

EID: 0037327444     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkg184     Document Type: Review

References (31)

1. Fire,A., Xu,S., Montgomery,M.K., Kostas,S.A., Driver,S.E. and Mello,C.C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391, 806-811.
2. Sharp,P.A. (2001) RNA interference-2001. Genes Dev., 15, 485-490.
3. Hutvagner,G. and Zamore,P.D. (2002) RNAi: nature abhors a double-strand. Curr. Opin. Genet. Dev., 12, 225-232.
4. Hannon,G.J. (2002) RNA interference. Nature, 418, 244-251.
5. Zamore,P., Tuschl,T., Sharp,P. and Bartel,D. (2000) RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21- to 23-nucleotide intervals. Cell, 101, 25-33.
6. Elbashir,S.M., Lendeckel,W. and Tuschl,T. (2001) RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev., 15, 188-200.
7. Bernstein,E., Caudy,A.A., Hammond,S.M. and Hannon,G.J. (2001) Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature, 409, 363-366.
8. Tuschl,T., Zamore,P.D., Lehmann,R., Bartel,D.P. and Sharp,P.A. (1999) Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev., 13, 3191-3197.
9. Hammond,S.M., Bernstein,E., Beach,D. and Hannon,G.J. (2000) An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature, 404, 293-296.
10. Hammond,S.M., Boettcher,S., Caudy,A.A., Kobayashi,R. and Hannon,G.J. (2001) Argonaute 2, a link between genetic and biochemical analysis of RNAi. Science, 293, 1146-1150.
11. Bernstein,E., Denli,A.M. and Hannon,G.J. (2001) The rest is silence. RNA, 7, 1509-1521.
12. Vaucheret,H., Beclin,C. and Fagard,M. (2001) Post-transcriptional gene silencing in plants. J. Cell Sci., 114, 3083-3091.
13. Hammond,S.M., Caudy,A.A. and Hannon,G.J. (2001) Post-transcriptional gene silencing by double-stranded RNA. Nature Rev. Genet., 2, 110-119.
14. Grishok,A. and Mello,C.C. (2002) RNAi (nematodes: Caenorhabditis elegans). Adv. Genet., 46, 339-360.
15. Billy,E., Brondani,V., Zhang,H., Müller,U. and Filipowicz,W. (2001) Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines. Proc. Natl Acad. Sci. USA, 98, 14428-14433.
16. Paddison,P.J., Caudy,A.A. and Hannon,G.J. (2002) Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl Acad. Sci. USA, 99, 1443-1448.
17. Stark,G.R., Kerr,I.M., Williams,B.R., Silverman,R.H. and Schreiber,R.D. (1998) How cells respond to interferons. Annu. Rev. Biochem., 67, 227-264.
18. Elbashir,S.M., Harborth,J., Lendeckel,W., Yalcin,A., Weber,K. and Tuschl,T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in mammalian cell culture. Nature, 411, 494-498.
19. Holen,T., Amarzguioui,M., Wiiger,M.T., Babaie,E. and Prydz,H. (2002) Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor. Nucleic Acids Res., 30, 1757-1766.
20. Yang,D., Buchholz,F., Huang,Z., Goga,A., Chen,C.Y., Brodsky,F.M. and Bishop,J.M. (2002) Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc. Natl Acad. Sci. USA, 99, 9942-9947.
21. Amarasinghe,A.K., Calin-Jageman,I., Harmouch,A., Sun,W. and Nicholson,A.W. (2001) Escherichia coli ribonuclease III: affinity purification of hexahistidine-tagged enzyme and assays for substrate binding and cleavage. Methods Enzymol., 342, 143-158.
22. Matsuda,S., Ichigotani,Y., Okuda,T., Irimura,T., Nakatsugawa,S. and Hamaguchi,M. (2000) Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase. Biochim. Biophys. Acta, 1490, 163-169.
23. Harborth,J., Elbashir,S.M., Bechert,K., Tuschl,T. and Weber,K. (2001) Identification of essential genes in cultured mammalian cells using small interfering RNAs. J. Cell Sci., 114, 4557-4565.
24. Provost,P., Dishart,D., Doucet,J., Frendewey,D., Samuelsson,B. and Radmark,O. (2002) Ribonuclease activity and RNA binding of recombinant human Dicer. EMBO J., 21, 5864-5874.
25. Zhang,H., Kolb,F.A., Brondani,V., Billy,E. and Filipowicz,W. (2002) Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP. EMBO J., 21, 5875-5885.
26. Zuker,M., Mathews,D.H. and Turner,D.H. (1999) Algorithms and thermodynamics for RNA secondary structure prediction: a practical guide. In Barciszewski,J. and Clark,B.F.C. (eds), RNA Biochemistry and Biotechnology. Kluwer Academic, Hingham, MA.
27. Adjei,A.A. (2001) Blocking oncogenic Ras signaling for cancer therapy. J. Natl Cancer Inst., 93, 1062-1074.
28. Miyagishi,M., Hayashi,M. and Taira,K. (2003) Comparison of the suppressive effects of antisense oligonucleotides and siRNAs directed against the same targets in mammalian cells. Antisense Nucleic Acid Drug Dev., in press.
29. Caplen,N.J., Fleenor,J., Fire,A. and Morgan,R.A. (2000) dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for the analysis of RNA interference. Gene, 252, 95-105.
30. Ngo,H., Tschudi,C., Gull,K. and Ullu,E. (1998) Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proc. Natl Acad. Sci. USA, 95, 14687-14692.
31. Sijen,T., Fleenor,J., Simmer,F., Thijssen,K.L., Parrish,S., Timmons,L., Plasterk,R.H. and Fire,A. (2001) On the role of RNA amplification in dsRNA-triggered gene silencing. Cell, 107, 465-476.