Signaling but not trafficking function of HIV-1 protein Nef is essential for Nef-induced defects in human intrathymic T-cell development

Blood

Volumn 102, Issue 8, 2003, Pages 2925-2932

  Stove, Veronique ^b   Naessens, Evelien ^b   Stove, Christophe ^b   Swigut, Tomek ^b   Plum, Jean ^b   Verhasselt, Bruno ^a  

^a GHENT UNIVERSITY HOSPITAL   (Belgium)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

NEF PROTEIN; P21 ACTIVATED KINASE 2; PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN 1; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ALLELE; ARTICLE; BINDING SITE; CASE REPORT; CELL FUNCTION; CELL MATURATION; CHILD; CONTROLLED STUDY; DISEASE ASSOCIATION; DISEASE COURSE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; HUMAN TISSUE; IMMUNOBIOLOGY; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN STRUCTURE; SIGNAL TRANSDUCTION; SRC HOMOLOGY DOMAIN; STRUCTURE ACTIVITY RELATION; T LYMPHOCYTE; THYMOCYTE; THYMUS;

EID: 0141993006     PISSN: 00064971     EISSN: None     Source Type: Journal    
DOI: 10.1182/blood-2003-03-0833     Document Type: Article

References (63)

1. UNAIDS/WHO. AIDS Epidemic Update: December 2002. Geneva, Switzerland, 2002.
2. + T-cell depletion in HIV disease. Nature. 2001;410:974-979.
3. Plum J, De Smedt M, Verhasselt B, et al. Human T lymphopoiesis: in vitro and in vivo study models. Ann N Y Acad Sci. 2000;917:724-731.
4. Spits H. Development of alphabeta T cells in the human thymus. Nat Rev Immunol. 2002;2:760-772.
5. Bonyhadi ML, Rabin L, Salimi S, et al. HIV induces thymus depletion in vivo. Nature. 1993; 363:728-732.
6. Su L, Kaneshima H, Bonyhadi M, et al. HIV-1-induced thymocyte depletion is associated with indirect cytopathogenicity and infection of progenitor cells in vivo. Immunity. 1995;2:25-36.
7. + cell depletion in human immunodeficiency virus type 1-infected SCID-hu mice suggest direct viral killing of thymocytes in vivo. J Virol. 1997;71:8245-8253.
8. Douek DC, Betts MR, Hill BJ, et al. Evidence for increased T cell turnover and decreased thymic output in HIV infection. J Immunol. 2001;167: 6663-6668.
9. + (T4) lymphocyte depletion. Proc Natl Acad Sci U S A. 1990;87:7727-7731.
10. Valentin H, Nugeyre MT, Vuillier F, et al. Two subpopulations of human triple-negative thymic cells are susceptible to infection by human immunodeficiency virus type 1 in vitro. J Virol. 1994;68: 3041-3050.
11. Deacon NJ, Tsykin A, Solomon A, et al. Genomic structure of an attenuated quasi species of HIV-1 from a blood transfusion donor and recipients. Science. 1995;270:988-991.
12. Kirchhoff F, Greenough TC, Brettler DB, Sullivan JL, Desrosiers RC. Brief report: absence of intact nef sequences in a long-term survivor with non-progressive HIV-1 infection. N Engl J Med. 1995; 332:228-232.
13. Jamieson BD, Aldrovandi GM, Planelles V, et al. Requirement of human immunodeficiency virus type 1 nef for in vivo replication and pathogenicity. J Virol. 1994;68:3478-3485.
14. Aldrovandi GM, Zack JA. Replication and pathogenicity of human immunodeficiency virus type 1 accessory gene mutants in SCID-hu mice. J Virol. 1996;70:1505-1511.
15. Duus KM, Miller ED, Smith JA, Kovalev GI, Su L. Separation of human immunodeficiency virus type 1 replication from nef-mediated pathogenesis in the human thymus. J Virol. 2001;75:3916-3924.
16. Verhasselt B, Naessens E, Verhofstede C, et al. Human immunodeficiency virus nef gene expression affects generation and function of human T cells, but not dendritic cells. Blood. 1999;94: 2809-2818.
17. Skowronski J, Parks D, Mariani R, Altered T cell activation and development in transgenic mice expressing the HIV-1 nef gene. EMBO J. 1993; 12:703-713.
18. Brady HJ, Pennington DJ, Miles CG, Dzierzak EA. CD4 cell surface downregulation in HIV-1 Nef transgenic mice is a consequence of intracellular sequestration. EMBO J. 1993;12:4923-4932.
19. Lindemann D, Wilhelm R, Renard P, Althage A, Zinkernagel R, Mous J. Severe immunodeficiency associated with a human immunodeficiency virus 1 NEF/3′-long terminal repeat transgene. J Exp Med. 1994;179:797-807.
20. Hanna Z, Kay DG, Rebai N, Guimond A, Jothy S, Jolicoeur P. Nef harbors a major determinant of pathogenicity for an AIDS-like disease induced by HIV-1 in transgenic mice. Cell. 1998; 95:163-175.
21. Arold ST, Baur AS. Dynamic Nef and Nef dynamics: how structure could explain the complex activities of this small HIV protein. Trends Biochem Sci. 2001;26:356-363.
22. Arold S, Hoh F, Domergue S, et al. Characterization and molecular basis of the oligomeric structure of HIV-1 nef protein. Protein Sci. 2000;9: 1137-1148.
23. Garcia JV, Miller AD. Serine phosphorylation-independent downregulation of cell-surface CD4 by nef. Nature. 1991;350:508-511.
24. Schwartz O, Marechal V, Le Gall S, Lemonnier F, Heard JM. Endocytosis of major histocompatibility complex class I molecules is induced by the HIV-1 Nef protein. Nat Med. 1996;2:338-342.
25. Harris M. HIV: a new role for Nef in the spread of HIV. Curr Biol. 1999;9:R459-R461.
26. Geyer M, Fackler OT, Peterlin BM. Structure-function relationships in HIV-1 Nef. EMBO Rep, 2001;2:580-585.
27. Mariani R, Skowronski J. CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals. Proc Natl Acad Sci U S A. 1993;90:5549-5553.
28. Aiken C, Konner J, Landau NR, Lenburg ME, Trono D. Nef induces CD4 endocytosis: requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain. Cell. 1994;76:853-864.
29. Mangasarian A, Piguet V, Wang JK, Chen YL, Trono D. Nef-induced CD4 and major histocompatibility complex class I (MHC-I) down-regulation are governed by distinct determinants: N-terminal alpha helix and proline repeat of Nef selectively regulate MHC-I trafficking. J Virol. 1999;73:1964-1973.
30. Piguet V, Wan L, Borel C, et al. HIV-1 Nef protein binds to the cellular protein PACS-1 to downregulate class I major histocompatibility complexes. Nat Cell Biol. 2000;2:163-167.
31. + viruses but not for down-regulation of CD4. EMBO J. 1995;14:484-491.
32. Greenberg M, DeTulleo L, Rapoport I, Skowronski J, Kirchhausen T. A dileucine motif in HIV-1 Nef is essential for sorting into clathrin-coated pits and for downregulation of CD4, Curr Biol. 1998;8:1239-1242.
33. Greenberg ME, Iafrate AJ, Skowronski J. The SH3 domain-binding surface and an acidic motif in HIV-1 Nef regulate trafficking of class I MHC complexes. EMBO J. 1998;17;2777-2789.
34. Iafrate AJ, Bronson S, Skowronski J. Separable functions of Nef disrupt two aspects of T cell receptor machinery: CD4 expression and CD3 signaling. EMBO J. 1997;16:673-684.
35. Sawai ET, Baur AS, Peterlin BM, Levy JA, Cheng Mayer C. A conserved domain and membrane targeting of Nef from HIV and SIV are required for association with a cellular serine kinase activity. J Biol Chem. 1995;270:15307-15314.
36. Liu LX, Heveker N, Fackler OT, et al. Mutation of a conserved residue (D123) required for oligomerization of human immunodeficiency virus type 1 Nef protein abolishes interaction with human thioesterase and results in impairment of Nef biological functions. J Virol. 2000;74:5310-5319.
37. Bresnahan PA, Yonemoto W, Ferrell S, Williams-Herman D, Geleziunas R, Greene WC. A dileucine motif in HIV-1 Nef acts as an internalization signal for CD4 downregulation and binds the AP-1 clathrin adaptor. Curr Biol. 1998;8:1235-1238.
38. Craig HM, Pandori MW, Guatelli JC. Interaction of HIV-1 Nef with the cellular dileucine-based sorting pathway is required for CD4 down-regulation and optimal viral infectivity. Proc Natl Acad Sci U S A. 1998;95:11229-11234.
39. Aiken C, Krause L, Chen YL, Trono D. Mutational analysis of HIV-1 Nef: identification of two mutants that are temperature-sensitive for CD4 downregulation. Virology. 1996;217:293-300.
40. Taghon T, Stolz F, De Smedt M, et al. HOX-A10 regulates hematopoietic lineage commitment: evidence for a monocyte-specific transcription factor. Blood. 2002;99:1197-1204.
41. Verhasselt B, De Smedt M, Verhelst R, Naessens E, Plum J. Retrovirally transduced CD34++ human cord blood cells generate T cells expressing high levels of the retroviral encoded green fluorescent protein marker in vitro. Blood. 1998;91: 431-440.
42. Bahnson AB, Dunigan JT, Baysal BE, et al. Centrifugal enhancement of retroviral mediated gene transfer. J Virol Methods. 1995;54:131-143.
43. Kienzle N, Freund J, Kalbitzer HR, Mueller Lantzsch N. Oligomerization of the Nef protein from human immunodeficiency virus (HIV) type 1. Eur J Biochem. 1993;214:451-457.
44. Fujii Y, Otake K, Fujita Y, et al. Clustered localization of oligomeric Nef protein of human immunodeficiency virus type 1 on the cell surface. FEBS Lett. 1996;395:257-261.
45. Renkema GH, Manninen A, Mann DA, Harris M, Saksela K. Identification of the Nef-associated kinase as p21-activated kinase 2. Curr Biol. 1999; 9:1407-1410.
46. Arora VK, Molina RP, Foster JL, et al. Lentivirus Nef specifically activates Pak2. J Virol. 2000;74: 11081-11087.
47. Manninen A, Huotari P, Hiipakka M, Renkema GH, Saksela K. Activation of NFAT-dependent gene expression by Nef: conservation among divergent Nef alleles, dependence on SH3 binding and membrane association, and cooperation with protein kinase C-theta. J Virol. 2001;75:3034-3037.
48. Walk SF, Alexander M, Maier B, Hammarskjold ML, Rekosh DM, Ravichandran KS. Design and use of an inducibly activated human immunodeficiency virus type 1 Nef to study immune modulation. J Virol. 2001;75:834-843.
49. Benson RE, Sanfridson A, Ottinger JS, Doyle C, Cullen BR. Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection. J Exp Med. 1993; 177:1561-1566.
50. Ross TM, Oran AE, Cullen BR. Inhibition of HIV-1 progeny virion release by cell-surface CD4 is relieved by expression of the viral Nef protein. Curr Biol. 1999;9:613-621.
51. Lama J, Mangasarian A, Trono D. Cell-surface expression of CD4 reduces HIV-1 infectivity by blocking Env incorporation in a Nef- and Vpu-inhibitable manner. Curr Biol. 1999;9:622-631.
52. Lundquist CA, Tobiume M, Zhou J, Unutmaz D, Aiken C. Nef-mediated downregulation of CD4 enhances human immunodeficiency virus type 1 replication in primary T lymphocytes. J Virol. 2002;76:4625-4633.
53. + individuals. Blood. 1998;92:198-206.
54. Livingstone WJ, Moore M, Innes D, Bell JE, Simmonds P. Frequent infection of peripheral blood CD8-positive T-lymphocytes with HIV-1. Edinburgh Heterosexual Transmission Study Group. Lancet. 1996;348:649-654.
55. Flamand L, Crowley RW, Lusso P, Colombini-Hatch S, Margolis DM, Gallo RC. Activation of CD8+ T lymphocytes through the T cell receptor turns on CD4 gene expression: implications for HIV pathogenesis. Proc Natl Acad Sci U S A. 1998;95:3111-3116.
56. Bosselut R, Feigenbaum L, Sharrow SO, Singer A. Strength of signaling by CD4 and CD8 coreceptor tails determines the number but not the lineage direction of positively selected thymocytes. Immunity. 2001;14:483-494.
57. Stoddart CA, Geleziunas R, Ferrell S, et al. Human immunodeficiency virus type 1 Nef-mediated downregulation of CD4 correlates with Nef enhancement of viral pathogenesis. J Virol. 2003; 77:2124-2133.
58. Fackler OT, Baur AS. Live and let die: Nef functions beyond HIV replication. Immunity. 2002;16: 493-497.
59. Grzesiek S, Bax A, Clore GM, et al. The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase. Nat Struct Biol. 1996;3:340-345.
60. Lee CH, Saksela K, Mirza UA, Chait BT, Kuriyan J. Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain. Cell. 1996;85:931-942.
61. Hanna Z, Weng X, Kay DG, Poudrier J, Lowell C, Jolicoeur P. The pathogenicity of human immunodeficiency virus (HIV) type 1 Nef in CD4C/HIV transgenic mice is abolished by mutation of its SH3-binding domain, and disease development is delayed in the absence of Hck. J Virol. 2001; 75:9378-9392.
62. Manninen A, Renkema GH, Saksela K. Synergistic activation of NFAT by HIV-1 nef and the Ras/MAPK pathway. J Biol Chem. 2000;275:16513-16517.
63. Kosugi A, Zuniga Pflucker JC, Sharrow SO, Kruisbeek AM, Shearer GM. Effect of cyclosporin A on lymphopoiesis, II: developmental defects of immature and mature thymocytes in fetal thymus organ cultures treated with cyclosporin A. J Immunol. 1989;143:3134-3140.