Mutational analysis of HIV-1 Nef: Identification of two mutants that are temperature-sensitive for CD4 downregulation

Virology

Volumn 217, Issue 1, 1996, Pages 293-300

  Aiken, Christopher ^a,b   Krause, Lisa ^a   Chen, Yen Liang ^a   Trono, Didier ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; NEF PROTEIN;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE SENSITIVE MUTANT; VIRUS MUTANT;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0029924038     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1996.0116     Document Type: Article

References (34)

1. Ahmad, N., and Venkatesan, S. (1988). Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR Science 241, 1481-1485.
2. Aiken, C., and Trono, D. (1995). Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis. J. Virol. 69, 5048-5056.
3. Aiken, C., Konner, J., Landau, N. R., Lenburg, M. E., and Trono, D. (1994). Nef induces CD4 endocytosis: Requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain. Cell 76, 853-864.
4. Anderson, S. J., Lenburg, M., Landau, N. R., and Garcia, J. V. (1994). The cytoplasmic domain of CD4 is sufficient for its down-regulation from the cell surface by human immunodeficiency virus type 1 Nef. J. Virol. 68, 3092-3101.
5. Chowers, M. Y., Spina, C. A., Kwoh, T. J., Fitch, N. J. S., Richman, D. D., and Guatelli, J. C. (1994). Optimal infectivity in vitro of human immunodeficiency virus type 1 requires an intact nef gene. J. Virol. 68, 2906-2914.
6. Chowers, M. Y., Pandori, M. W., Spina, C. A., Richman, D. D., and Guatelli, J. C. (1995). The growth advantage conferred by HIV-1 nef is determined at the level of viral DNA formation and is independent of CD4 downregulation. Virology 212, 451-457.
7. de Ronde, A., Klaver, B., Keulen, W., Smit, L., and Goudsmit, J. (1992). Natural HIV-1 Nef accelerates virus replication in primary human lymphocytes. Virology 188, 391-395.
8. Garcia, J. V., and Miller, A. D. (1991). Serine phosphorylation-independent downregulation of cell-surface CD4 by nef. Nature 350, 508-511.
9. Gibbs, C. S., and Zoller, M. J. (1991). Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions. J. Biol. Chem. 266, 8923-8931.
10. Goldsmith, M. A., Warmerdam, M. T., Atchison, R. E., Miller, M. D., and Greene, W. C. (1995). Dissociation of the CD4 downregulation and viral infectivity enhancement functions of human immunodeficiency virus type 1 Nef. J. Virol. 69, 4112-4121.
11. Guy, B., Kieny, M. P., Riviere, Y., Le Peuch, C., Dott, K., Girard, M., Montagnier, L., and Lecocq, J. P. (1987). HIV F/3́ orf encodes a phosphorylated GTP-binding protein resembling an oncogene product. Nature 330, 266-269.
12. Hammes, S. R., Dixon, E. P., Malim, M. H., Cullen, B. R., and Greene, W. C. (1989). Nef protein of human immunodeficiency virus type 1: Evidence against its role as a transcriptional inhibitor. Proc. Natl. Acad. Sci. USA 86, 9549-9553.
13. Harris, M. P., and Neil, J. C. (1994). Myristoylation-dependent binding of HIV-1 Nef to CD4. J. Mol. Biol. 241, 136-142.
14. Jamieson, B. D., Aldrovandi, G. M., Planelles, V., Jowett, J. B. M., Gao, L., Bloch, L. M., Chen, I. S. Y., and Zack, J. A. (1994). Requirement of human immunodeficiency virus type 1 nef for in vivo replication and pathogenicity. J. Virol. 68, 3478-3485.
15. Kestler, H. W., III, Ringler, D. J., Mori, K., Panicali, D. L., Sehgal, P. K., Daniel, M. D., and Desrosiers, R. C. (1991). Importance of the nef gene for maintenance of high virus loads and for development of AIDS. Cell 65, 651-662.
16. Kim, S., Ikeuchi, K., Byrn, R., Groopman, J., and Baltimore, D. (1989). Lack of a negative influence on viral growth by the nef gene of human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA 86, 9544-9548.
17. Kunkel, T. A. (1985). Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82, 488-492.
18. Luciw, P. A., Cheng-Mayer, C., and Levy, J. A. (1987). Mutational analysis of the human immunodeficiency virus: The orf-B region down-regulates virus replication. Proc. Natl. Acad. Sci. USA 84, 1434-1438.
19. Mariani, R., and Skowronski, J. (1993). CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals. Proc. Natl. Acad. Sci. USA 90, 5549-5553.
20. Miller, M. D., Warmerdam, M. T., Gaston, I., Greene, W. C., and Feinberg, M. B. (1994). The human immunodeficiency virus-1 nef gene product: A positive factor for viral infection and replication in primary lymphocytes and macrophages. J. Exp. Med. 179, 101-113.
21. Miller, M. D., Warmerdam, M. T., Page, K. A., Feinberg, M. B., and Greene, W. C. (1995). Expression of the human immunodeficiency virus type 1 (HIV-1) nef gene during HIV-1 production increases progeny particle infectivity independently of gp160 or viral entry. J. Virol. 69, 579-584.
22. Niederman, T. M. J., Thielan, B. J., and Ratner, L. (1989). Human immunodeficiency virus type 1 negative factor is a transcriptional silencer. Proc. Natl. Acad. Sci. USA 86, 1128-1132.
23. Ren, R., Mayer, B. J., Cicchetti, P., and Baltimore, D. (1993). Identification of a ten-amino acid proline-rich SH3 binding site. Science 259, 1157-1161.
24. Rhee, S. S., and Marsh, J. W. (1994). Human immunodeficiency virus type 1 nef-induced down-modulation of CD4 is due to rapid internalization and degradation of surface CD4. J. Virol. 68, 5156-5163.
25. + viruses but not down-regulation of CD4. EMBO J. 14, 484-491.
26. Sawai, E. T., Baur, A. S., Peterlin, B. M., Levy, J. A., and Cheng-Mayer, C. (1995). A conserved domain and membrane targeting of Nef from HIV and SIV are required for association with a cellular serine kinase activity. J. Biol. Chem. 270, 15307-15314.
27. Schwartz, O., Marechal, V., Danos, O., and Heard, J.-M. (1995). Human immunodeficiency virus type 1 Nef increases the efficiency of reverse transcription in the infected cell. J. Virol. 69, 4053-4059.
28. Shugars, D. C., Smith, M. S., Glueck, D. H., Nantermet, P. V., Seillier-Moiseiwitsch, F., and Swanstrom, R. (1993). Analysis of human immunodeficiency virus type 1 nef gene sequences present in vivo. J. Virol. 67, 4639-4650.
29. Spina, C. A., Kwoh, T. J., Chowers, M. Y., Guatelli, J. C., and Richman, D. D. (1994). The importance of nef in the induction of human immunodeficiency virus type 1 replication from primary quiescent CD4 lymphocytes. J. Exp. Med. 179, 115-123.
30. Terwilliger, E. F., Langhoff, E., Gabuzda, D., Zazopoulos, E., and Haseltine, W. A. (1991). Allelic variation in the effects of the nef gene on replication of human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA 88, 10971-10975.
31. 3 receptors. Cell 65, 1255-1266.
32. Yu, H., Chen, J. K., Feng, S., Dalgarno, D. C., Brauer, A. W., and Schreiber, S. L. (1994). Structural basis for the binding of proline-rich peptides to SH3 domains. Cell 76, 933-945.
33. Zazopoulos, E., and Haseltine, W. A. (1992). Mutational analysis of the human immunodeficiency virus type 1 Eli Nef function. Proc. Natl. Acad. Sci. USA 89, 6634-6638.
34. Zazopoulos, E., and Haseltine, W. A. (1993). Disulfide bond formation in the human immunodeficiency virus type 1 Nef protein. J. Virol. 67, 1676-1680.