HIV-1 Nef protein binds to the cellular protein PACS-1 to downregulate class I major histocompatibility complexes

Nature Cell Biology

Volumn 2, Issue 3, 2000, Pages 163-167

  Piguet, Vincent ^a   Wan, Lei ^b   Borel, Christelle ^a   Mangasarian, Aram ^a   Demaurex, Nicolas ^a   Thomas, Gary ^b   Trono, Didier ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CELL PROTEIN; CHIMERIC PROTEIN; FURIN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; MEMBRANE PROTEIN; NEF PROTEIN; PROTEIN PACS 1; UNCLASSIFIED DRUG;

ARTICLE; CELL SURFACE; CONTROLLED STUDY; CYTOPLASM; CYTOTOXIC T LYMPHOCYTE; DOWN REGULATION; GOLGI COMPLEX; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; IMMUNOREGULATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING;

ANIMALS; ANTIGENS, CD4; CAPSID; CARRIER PROTEINS; CELL LINE; DOWN-REGULATION; ENDOCYTOSIS; FURIN; GENE EXPRESSION REGULATION; GENE PRODUCTS, NEF; GOLGI APPARATUS; HISTOCOMPATIBILITY ANTIGENS CLASS I; HIV-1; HUMANS; HYDROGEN-ION CONCENTRATION; MELANOMA; MEMBRANE PROTEINS; OLIGONUCLEOTIDES, ANTISENSE; PROTEIN STRUCTURE, TERTIARY; RATS; RECEPTOR, IGF TYPE 2; RECOMBINANT FUSION PROTEINS; SUBTILISINS; TRANSFECTION; VESICULAR TRANSPORT PROTEINS;

EID: 0033776709     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/35004038     Document Type: Article

References (25)

1. Endocytosis of major histocompatibility complex class I molecules is induced by the HIV-1 Nef protein
2. HIV-1 Nef protein protects infected primary cells against killing by cytotoxic T lymphocytes
3. The SH3 domain-binding surface and an acidic motif in HIV-1 Nef regulate trafficking of class I MHC complexes
4. PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization
5. Mutational analysis of HIV-1 Nef: Identification of two mutants that are temperature-sensitive for CD4 downregulation
6. Cell-surface expression of CD4 reduces HIV-1 infectivity by blocking env incorporation in a nef- and vpu-inhibitable manner
7. Inhibition of HIV-1 progeny virion release by cell-surface CD4 is relieved by expression of the viral nef protein
8. Nef induces CD4 endocytosis: Requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain
9. Nef-induced CD4 degradation: A diacidic-based motif in Nef functions as a lysosomal targeting signal through the binding of β-COP in endosomes
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11. Mechanism of Nef in duced CD4 endocytosis: Nef connects CD4 with the μ chain of adaptor complexes
12. Nef interacts with the μ subunit of clathrin adaptor complexes and reveals a cryptic sorting signal in MHC-I molecules
13. Co-localization of HIV-1 Nef with the AP-2 adaptor protein complex correlates with Nef-induced CD4 down-regulation
14. The HIV-1 Nef protein acts as a connector with sorting pathways in the Golgi and at the plasma membrane
15. Interaction of HIV-1 nef with the cellular dileucine-based sorting pathway is required for CD4 down-regulation and optimal viral infectivity
16. A dileucine motif in HIV-1 Nef acts as an internalization signal for CD4 downregulation and binds the AP-1 clathrin adaptor
17. A dileucine motif in HIV-1 Nef is essential for sorting into clathrin-coated pits and for downregulation of CD4
18. Mechanism of acidification of the trans-Golgi network (TGN). In situ measurements of pH using retrieval of TGN38 and furin from the cell surface
19. Endocytosis
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21. The N-terminus of Nef from HIV-1/SIV associates with a protein complex containing Lck and a serine kinase
22. Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for downregulation of CD4
23. The CD4 determinant for downregulation by HIV-1 Nef directly binds to Nef. Mapping of the Nef binding surface by NMR
24. Viral strategies of immune evasion
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