Structure-function relationships in HIV-1 Nef

EMBO Reports

Volumn 2, Issue 7, 2001, Pages 580-585

  Geyer, Matthias ^a   Fackler, Oliver T ^b   Peterlin, B Matija ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; NEF PROTEIN; PROTEIN P21;

CONTROLLED STUDY; CORRELATION FUNCTION; DOWN REGULATION; HOST CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN PROCESSING; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; REVIEW; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; SIMIAN IMMUNODEFICIENCY VIRUS; STRUCTURE ACTIVITY RELATION; SURVIVAL; VIRUS PATHOGENESIS;

AMINO ACID MOTIFS; BINDING SITES; GENE PRODUCTS, NEF; HIV-1; HUMANS; MODELS, MOLECULAR; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, HIV; SIGNAL TRANSDUCTION; STRUCTURE-ACTIVITY RELATIONSHIP;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES; SIMIAE; SIMIAN IMMUNODEFICIENCY VIRUS;

EID: 0034894379     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1093/embo-reports/kve141     Document Type: Review

References (43)

1. Mutational analysis of HIV-1 Nef: Identification of two mutants that are temperature-sensitive for CD4 downregulation
2. The crystal structure of HIV-1 Nef protein bound to the Fyn kinase SH3 domain suggests a role for this complex in altered T cell receptor signaling
3. Physical interaction of the HIV-1 Nef protein with β-COP, a component of non-clathrin-coated vesicles essential for membrane traffic
4. Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection
5. A dileucine motif in HIV-1 Nef acts as an internalization signal for CD4 downregulation and binds the AP-1 clathrin adaptor
6. The human thioesterase II protein binds to a site on HIV 1 Nef critical for CD4 downregulation
7. HIV-1 Nef protein protects infected primary cells against killing by cytotoxic T lymphocytes
8. Interaction of HIV-1 Nef with the cellular dileucine-based sorting pathway is required for CD4 down-regulation and optimal viral infectivity
9. Activation of Vav by Nef induces cytoskeletal rearrangements and downstream effector functions
10. P21-activated kinase 1 plays a critical role in cellular activation by Nef
11. A possible regulation of negative factor (Nef) activity of human immunodeficiency virus type 1 by the viral protease
12. Domain assembly, surface accessibility and sequence conservation in full length HIV-1 Nef
13. Structure of the anchor-domain of myristoylated and non-myristoylated HIV-1 Nef protein
14. A dileucine motif in HIV-1 Nef is essential for sorting into clathrin-coated pits and for downregulation of CD4
15. The CD4 determinant for downregulation by HIV-1 Nef directly binds to Nef. Mapping of the Nef binding surface by NMR
16. Refined solution structure and backbone dynamics of HIV-1 Nef
17. Mutational analysis of the HIV Nef protein
18. The role of myristoylation in the interactions between human immuno-deficiency virus type 1 Nef and cellular proteins
19. Binding of the c-Raf1 kinase to a conserved acidic sequence within the carboxyl-terminal region of the HIV-1 Nef protein
20. Human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus Nef use distinct but overlapping target sites for downregulation of cell surface CD4
21. Separable functions of Nef disrupt two aspects of T cell receptor machinery: CD4 expression and CD3 signalling
22. HIV-1 Nef plays an essential role in two independent processes in CD4 down-regulation: Dissociation of the CD4-p56(lck) complex and targeting of CD4 to lysosomes
23. Cell-surface expression of CD4 reduces HIV-1 infectivity by blocking Env incorporation in a Nef- and Vpu-inhibitable manner
24. Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in Rhesus macaques
25. Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain
26. Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains
27. Mutation of a conserved residue (D123) required for oligomerization of human immunodeficiency virus type 1 Nef protein abolishes interaction with human thioesterase and results in impairment of Nef biological functions
28. Interactions between HIV-1 Nef and vacuolar ATPase facilitate the internalization of CD4
29. SH3-domain binding function of HIV-1 Nef is required for association with a PAK-related kinase
30. The structural era of endocytosis
31. Bi-cycling the furin pathway: From TGN localization to pathogen activation and embryogenesis
32. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons
33. Producer-cell modification of human immunodeficiency virus type 1: Nef is a virion protein
34. Nef-induced CD4 degradation: A diacidic-based motif in Nef functions as a lysosomal targeting signal through the binding of β-COP in endosomes
35. HIV-1 Nef protein binds to the cellular protein PACS-1 to downregulate class I major histocompatibility complexes
36. Identification of the Nef-associated kinase as p21-activated kinase 2
37. Fatty acylation of proteins: New insights into membrane targeting of myristoylated and palmitoylated proteins
38. Inhibition of HIV-1 progeny virion release by cell-surface CD4 is relieved by expression of the viral Nef protein
39. + viruses but not for downregulation of CD4
40. A conserved domain and membrane targeting of Nef from HIV and SIV are required for association with a cellular serine kinase activity
41. Activation of PAK by HIV and SIV Nef: Importance for AIDS in Rhesus macaques
42. Human immunodeficiency virus type 1 Nef protein is incorporated into virus particles and specifically cleaved by the viral proteinase
43. Induction of Fas ligand expression by HIV involves the interaction of Nef with the T cell receptor ζ chain