Apoptotic detection methods - From morphology to gene

Progress in Histochemistry and Cytochemistry

Volumn 38, Issue 3, 2003, Pages 275-339

  Otsuki, Yoshinori ^a   Li, Zhonglian ^a   Shibata, Masa Aki ^a  

^a OSAKA MEDICAL COLLEGE   (Japan)

Author keywords

Apoptosis; Bcl 2; Bcl 2 transcriptional factors; Caspase activity; Confocal laser microscopy; DNA agarose gel electrophoresis; Fas; Fas ligand; Flow cytometry; Immunoelectron microscopy; Immunohistochemistry; Necrosis; Real time Southwestern technique; RT PCR

Indexed keywords

CASPASE; EOSIN; FAS LIGAND; FLUORESCENT DYE; HEMATOXYLIN; PROTEIN BCL 2; TOLONIUM CHLORIDE; TUMOR NECROSIS FACTOR; TUMOR NECROSIS FACTOR RECEPTOR;

AGAR GEL ELECTROPHORESIS; ANTIBODY SPECIFICITY; APOPTOSIS; CELL BUDDING; CELL DEATH; CELL STRUCTURE; CELLULAR DISTRIBUTION; CHROMATIN CONDENSATION; COLORIMETRY; CONFOCAL LASER MICROSCOPY; ENDOMETRIUM; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUOROMETRY; GENE THERAPY; GENE TRANSFER; HUMAN; IMMUNOELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; NECROSIS; NICK END LABELING; NONHUMAN; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; SIGNAL TRANSDUCTION; SOUTHWESTERN BLOTTING; STAINING; TRANSCRIPTION REGULATION; TRANSMISSION ELECTRON MICROSCOPY; WESTERN BLOTTING;

EID: 0038407572     PISSN: 00796336     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0079-6336(03)80002-5     Document Type: Review

References (160)

1. AATSINKI, J. T., LAKKAKORPI, J. T., PIETILÄ, M. E., RAJANIEMI, H. J.: A coupled one-step reverse transcription PCR procedure for generation of full-length open reading frames. - BioTechniques 16, 282-288 (1994).
2. ADAMS, J. M., CORY, S.: The Bcl-2 protein family: arbiters of cell survival. - Science 281, 1322-1326 (1998).
3. AKAO, Y., OTSUKI, Y., KATAOKA, S., ITO, Y., TSUJIMOTO, Y.: Multiple subcellular localization of bcl-2: Detection in nuclear outer membrane, endoplasmic reticulum membrane, and mitochondrial membrane. - Cancer Res. 54, 2468-2471 (1994).
4. ANSALI, B., COATES, P. J., GREENSTEIN, B. D., HALL, P. A.: In situ end-labelling detects DNA strand breaks in apoptosis and other physiological and pathological stages. - J. Pathol. 170, 1-8 (1993).
5. ARAI, H., GORDON, D., NAVEL, E. G., and NAVEL, G. J.: Gene transfer of Fas lignad induces tumor regression in vivo. - Proc. Natl. Acd. Sci. USA 94, 13862-13867 (1997).
6. ARENDS, M. J., MORRIS, R. G., WYLLIE, A. H.: Apoptosis - The role of the endonuclease. - Am. J. Pathol. 136, 593-608 (1990).
7. BAKER, J. R., BRETZ, J. D.: Specificity questions concerning the clone 33 anti-fas ligand antibody. - Cell Death Differ. 7, 8-9 (2000).
8. BELLGRAU, D., GOLD, D., SELAWRY, H., MOORE, J., FRANZUSOFF, A., DUKE, R. C.: A role for CD95 ligand in preventing graft rejection. - Nature 377, 630-632 (1995).
9. BEVERLEY, S. M.: Enzymatic amplification of RNA by PCR (RT-PCR). In: Current Protocols in Molecular Biology. - Wiley & Sons, New York (2001).
10. BURRES, N. S., FRIGO, A., RASMUSSEN, R. R., MCALPINE, J. B.: A colorimetric microassay for the detection of agents that interact with DNA. - J. nat. Prod. 55, 1582-1587 (1992).
11. BURSCH, W., KLEINE, L., TENNISWOOD, M.: The biochemistry of cell death by apoptosis. - Biochem. Cell Biol. 68, 1071-1074 (1990a).
12. BURSCH, W., PAFFE, S., PUTZ, B., SCHULTE-HERAMANN, R.: Determination of the length of the histological stages of apoptosis in normal liver and altered hepatic foci of rats. - Carcinogenesis 11, 847-853 (1990b).
13. CHEN, H. M., BOXER, L. M.: Pi 1 binding sites are negative regulators of bcl-2 expression in pre-B cells. - Mol. Cell. Biol. 15, 3840-3847 (1995).
14. COHEN, G. M.: Caspases: the executioners of apoptosis. - Biochem. J. 326, 1-16 (1997).
15. COLL, J. L., NEGOESCU, A., LOUIS, N., SACHS, L., TENAUD, C., GIRARDOT, V., DEMEINEX, B., BRABILLA, E., BRABILLA, C., FAVROT, M.: Antitumor activity of bax and p53 naked gene transfer in lung cancer: in vivo analysis. - Hum. Gene Ther. 20, 2063-2074 (1998).
16. DAIKOKU, E., ITO, Y., OTSUKI, Y.: The induction of apoptosis in ovaries and uteri of bcl-2 deficient mice. - Med. Electron. Micros. 31, 68-76 (1998).
17. DALES, J. P., PALMERINI, F., DEVILARD, E., HASSOUN, J., BIRG, F., XERRI, L.: Caspases: conductors of the cell death machinery in lymphoma cells. - Leuk. Lymphoma 41, 247-253 (2001).
18. DARZYNKIEWICZ, Z., BENDER, E., BURFEIND, P., TRAGANOS, F.: Analysis of apoptosis by flow and laser scanning cytometry, Apoptosis Detection and Assay Methods.-Eaton Publishing, Natick, MA, USA (1998).
19. DARZYNKIEWICZ, Z., BENDER, E., LI, X., GORCZYCA, W., MELAMED, M. R.: Laser-scanning cytometry: a new instrumentation with many applications. - Exp. Cell. Res. 249, 1-12 (1999).
20. DARZYNKIEWICZ, Z., TRAGANOS, F., KAPUSCINSKI, J., STAIANO-COICO, L., MELAMED, M. R.: Accessibility of DNA in situ to various fluorochromes: relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. - Cytometry 5, 355-363 (1984).
21. DONG, L., WANG, W., WANG, F., SSONER, M., REED, J. C., HARIGAI, M., SAMUDIO, I., KLADDE, M. P., VYHLIDAL, C., SAFE, S.: Mechanisms of transcriptional activation of bcl-2 gene expression by 17beta-estradiol in breast cancer cells. - J. Biol. Chem. 274, 32099-32107 (1999).
22. DOUGLAS, R. G., CARL, F. W.: Fas-ligand: Privilege and peril. - Proc. Natl. Acad. Sci. USA 94, 5986-5990 (1997).
23. EID, N. A. S., ITO, Y., LI, Z. L., ABE, H., KUSAKABE, K., SHIBATA, M-A., OTSUKI, Y.: The relationship between apoptosis and splenocyte deletion in rats following ethanol treatment. - Med. Electron. Microsc. 33, 89-95 (2000).
24. EID, N. A. S., ITO, Y., SHIBATA, M-A., KUSAKABE, K., ABE, H., LI, Z. L., HORIGUCHI, T., OTSUKI, Y.: Sertoli cell injury in ethanol-treated rats: role of p53. - Proc. the 7th Int. Congress Androl. 349-355 (2001).
25. EID, N. A. S., SHIBATA, M-A., ITO, Y., KUSAKABE, K., HAMMAD, H., OTSUKI, Y.: Involvement of Fas system and active caspases in apoptotic signaling in testicular germ cells of ethanol-treated rats. - Int. J. Androl. 25, 159-167 (2002).
26. ENARI, M., HASE, A., NAGATA, S.: Apoptosis by cytosolic extract from Fas-activated cells. EMBO. J. 14, 5201-5208 (1995).
27. ENARI, M., TALANIAN, R. V., WONG, W. W., NAGATA, S.: Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis. - Nature 380, 723-726 (1996).
28. FADOK, V. A., VOELKER, D. R., CAMPBELL, P. A., COHEN, J. J., BRATTON, D. L., HENSON, P. M.: Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. - J. Immunol. 148, 2207-2216 (1992).
29. FADOK, V. A., LASZLO, D. J., NOBEL, P. W., WEINSTEIN, L., RICHES, D. W. H., HENSON, P. M. J.: Particle digestibility is required for induction of the phosphatidylserine recognition mechanism used by murine macrophages to phagocytose apoptotic cells. - J. Immunol. 151, 4274-4285 (1993).
30. FARQUHAR, M. G., PALADE, G. E.: The Golgi apparatus (complex)-(1954-1981)-from artifact to center Stage. - J. Cell Biol. 91, 77-103 (1981).
31. FERNANDES-ALNEMRI, T., LITWACK, G., ALNEMRI, E. S.: CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein and mammalian interleukin-1βconverting enzyme. - J. biol. Chem. 269, 30761-30764 (1994).
32. FIEDLER, P., EIBEL, H.: Antibody in Ab33 from Transduction laboratories detects human CD95L in ELISA but not in immunoblots. - Cell Death Differ. 7, 126-128 (2000).
33. FILLE, M., SHANLEY, J. D., ASLANZADEH, J.: Quantitative RT-PCR using a PCR-generated competitive internal standard. - BioTechniques 23, 34-36 (1997).
34. FISHER, R. J., FIVASH, M., CASAS-FINET, J., ERICKSON, J. W., KONDOH, A., BLADEN, S. V., FISHER, C., WATSON, D. K., PAPAS, T.: Real-time DNA binding measurements of the ETS1 recombinant oncoproteins reveal significant kinetic differences between the p42 and p51 isoforms. - Protein Sci. 3, 257-266 (1994).
35. FIVASH, M., TOWLER, E. M., FISHER, R. J.: BIAcore for macromolecular interaction. - Curr. Opin. Biotechnol. 9, 97-101 (1998).
36. FRAMPTON, J., RAMQVIST, T., GRAF T.: v-Myb of E26 leukemia virus up-regulates bcl-2 and suppresses apoptosis in myeloid cells. - Genes Dev. 10, 2720-2731 (1996).
37. FLEMMING, W.: Uber die Bildung von Richtungsfiguren in Saugethiereiern beim Untergang Graaf'scher Follikel. - Arch. Anat. Entw. Gesch. 221-244 (1885).
38. FRANKLIN, A. L., FILION, W. G.: Acridine orange-methyl green fluorescent staining of nucleoli. Stain. Technol. 56, 343-348 (1981).
39. FRENCH, L. E., HAHNE, M., VIARD, I., RADLGRUBER, G., ZANONE, R., BECHER, K., MULLER, C., TSCHOPP, J.: Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover. - J. Cell Biol. 133, 335-343 (1996).
40. GALLAGHER, S., WINSTON, S. E., FULLER, S. A., HURRELL, G. R.: Immunoblotting and imunodetection, Current Protocols in Molecular Biology.-Wiley & Sons, NY (1997).
41. GALIO, L., BRIQUET, S., COT, S., GUILLET, J. G., VAQUERO, C.: Analysis of interactions between huGATA-3 transcription factor and three GATA regulatory elements of HIV-1 long terminal repeat, by surface plasmon resonance. - Anal. Biochem. 253, 70-77 (1997).
42. GALIO, L., BRIQUET, S., VAQUERO, C.: Real-time study of interactions between a composite DNA regulatory region (HIV-1 LTR NRE) and several transcription factors of nuclear extracts. Biochem Biophys. Res. Commun. 264, 6-13 (1999).
43. GARCIA, I., MARTINOU, I., TSUJIMOTO, Y., MARTINOU, J. C.: Prevention of programmed cell death of sympathetic neurons by the bcl-2 proto-oncogene. - Science 258, 303-304 (1992).
44. GAVRIELI, Y., SHERMAN, Y., BEN-SASSON, S. A.: Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. - J. Cell Biol. 119, 493-501 (1992).
45. GLÜCKSMANN, A.: Cell deaths in normal vertebrate ontogeny. - Biol. Rev. Camb. Philps. Soc. 26, 59-86 (1951).
46. GOBLET, C., PROST, E., WHALEN, R. G.: One-step amplification of transcripts in total RNA using the polymerase chain reaction. - Nucleic Acids Res. 17, 2144 (1989).
47. GOLD, R., SCHMIED, M., ROTHE, G., ZISCHLER, H., WEKERLE, H., LASSMANN, H.: Detection of DNA fragmentation in apoptosis: application of in situ nick translation to cell culture systems and tissue sections. - J. Histochem. Cytochem. 41, 1023-1030 (1993).
48. GOLD, R., SCHMIED, M., GIEGERICH, G., BREITSCHOPF, H., HARTUNG, H. P., TOYKA, K. V., LASSMANN, H.: Differentiation between cellular apoptosis and necrosis by combined use of in situ tailing and nick translation techniques. - Lab. Invest. 71, 219-225 (1994).
49. GOMEZ-MANZANO, C., MITLIANGA, P., FUEYO, J., LEE, H.Y., Hu, M., SPURGERS, K. B., GLASS, T. L., KOUL, D., LIU, T. J., MCDONNELL, T. J., YUNG, W. K.: Transfer of E2F-1 to human glioma cells results in transcriptional up-regulation of Bcl-2. - Cancer Res. 61, 6693-6697 (2001).
50. GONG, J., TRAGANOS, F., DARZYNKIEWICZ, Z.: A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. - Anal. Biochem. 218, 314-319 (1994).
51. GOOSEN, M., BUJARD, H.: Tight control of gene expression in mammalian cells by tetracyclineresponsive promoter. - Proc. Natl. Acad. Sci. USA 89, 5547-5551 (1992).
52. GORCZYCA, W., GONG, J., DARZYNKIEWICZ, Z.: Detection of DNA strand breaks in individual apoptotic cell by the in situ terminal deoxynucleotidyl transferase and nick translation assays. - Cancer Res. 53, 1945-1951 (1993).
53. GRASSILLI, E., SALOMONI, P., PERROTTI, D., FRANCESCHI, C., CALABRETTA, B.: Resistance to apoptosis in CTLL-2 cells overexpressing B-Myb is associated with B-Myb-dependent bcl-2 induction. - Cancer Res. 59, 2451-2456 (1999).
54. GRIFFITH, T. S., BRUNNER, T., FLETCHER, S. M., GREEN, D. R., FERGUSON, T. A.: Fas ligand-induced apoptosis as a mechanism of immune privilege. - Science 270, 1189-1192 (1995).
55. GRIFFITH, T. S., FERGUSON, T. A.: The role of FasL-induced apoptosis in immune privilege. - Immunol. Today 18, 240-244 (1997).
56. GRIFFITHS, G., SIMONS, K.: The trans Golgi network: Sorting at the exit site of the Golgi complex. - Science 234, 438-443 (1986).
57. GURTU, V., Kain, S. R., ZHANG, G.: Fluorometric and colorimetric detection of caspase activity associated with apoptosis. - Anal. Biochem. 251, 98-102 (1997).
58. HAYASHI, R., ITO, Y., MATSUMOTO, K., FUJINO, Y., OTSUKI, Y.: Quantitative differentiation of both free 3′-OH and 5′-OH DNA ends between heat-induced apoptosis and necrosis. - J. Histochem. Cytochem. 46, 1051-1059 (1998).
59. Hayat, M. A.: Immunogold-silver staining overview. - J. Histotechnol. 16, 197-199 (1993).
60. HENGARTNER, O. M.: The biochemistry of apoptosis. - Nature 407, 770-771 (2000).
61. HOCHENBERY, D., NUNEZ, G., MILLIMAN, C., SCHRELBER, R. D., KORSMEYER, S. J.: bcl-2 is an inner membrane protein that blocks programmed cell death. - Nature 348, 334-336 (1990).
62. HOCKENBERY, D., ZUTTER, M., HICKER, W., NAHM, M., KORSMEYER, S. J.: Bcl2 protein is topographically restricted in tissues characterized by apoptotic cell death. - Proc. Natl. Acad. Sci. USA 88, 6961-6965 (1991).
63. HUANG, H., CHEVILLE, J. C., PAN, Y., ROCHE, P. C., SCHMIDT, L. J., TINDALL, D. J.: PTEN induces chemosensitivity in PTEN-mutated prostate cancer cells by suppression of Bcl-2 expression. -J. Biol. Chem. 276, 38830-38836 (2001).
64. INOKI, C., ITO, Y., YAMASHITA, H., UEKI, K., KUMAGAI, K., FUKUDA, Y., NINOMIYA, E., NAKAMURA, K., HAYASHI, R., UEKI, K., OTSUKI, Y.: Image analysis and ultrastructural detection of DNA strand breaks in human endometrium by in situ end-labeling techniques. - J. Histotechnol. 20, 321-328 (1997).
65. ITO, Y., OTSUKI, Y.: Localization of apoptotic cells in the human epidermis by an in situ DNA nick end-labeling method using confocal reflectant laser microscopy. - J. Histochem. Cytochem. 46, 783-786 (1998).
66. ITOH, N., YONEHARA, S., ISHII, A., MIZUSHIMA, S. I., SAMEJIMA, M., HASE, A., SETO, Y., NAGATA, S.: The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. - Cell 66, 233-243 (1991).
67. KAYAGAKI, N., KAWASAKI, A., EBATA, T., OHMOTO, H., IKEDA, S., INOUE, S., YOSHINO, K., OKUMURA, K., YAGITA, H.: Metalloproteinase-mediated release of human Fas ligand. -J. Exp. Med. 182, 1777-1783 (1995).
68. KERR, J. F. R., WYLLIE, A. H., CURRIE, A. R.: A basic biological phenomenon with wide-ranging implications in tissue kinetics. - Brit. J. Cancer 26, 239-257 (1972).
69. KIEFER, M. C., BRAUER, M. J., POWERS, V. C., WU, J. J., UMANSKY, S. R., TOMEI, L. D., BARR, P. J.: Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak. - Nature (Lond.) 374, 736-739 (1995).
70. KINGSTON, R. E., CHEN, C. A., OKAYAMA, H., ROSE, J. K.: Transfection of DNA into eukaryotic cells, Current Protocols in Molcular Biology.-Wiley & Sons, NY (1996).
71. KONDO, S., BARNA, B. P., MORIMURA, T., TAKEUCHI, J., YUAN, J., AKBASAK, A., BARTNETT, G. H.: Interleukin-1 beta-converting enzyme mediates cisplatin-induced apoptosis in malignant glioma cells. - Cancer Res. 55, 6166-6171 (1995).
72. KUMAGAI, K., OTSUKI, Y., ITO, Y., SHIBATA, M-A., ABE, H., UEKI, M.: Apoptosis in the normal human amnion at term, independent of Bcl-2 regulation and onset of labour. - Mol. Hum. Reprod. 7, 681-689 (2001).
73. LAERUM, O. R.: Flow cytometry, Encyclopedia of Human Biology.-Academic Press, New York (1991).
74. LEIST, M., SINGLE, B., CASTOLDI, A. F., KUHNLE, S., NOCOTERA, P.: Intracellular adenosine triphosphate (ATP) concentration: A switch in the decision between apoptosis and necrosis. - J. Exp. Med. 185, 1481-1486 (1997).
75. LEIST, M., JÄÄTTELÄ, M.: Four deaths and a funeral: from caspases to alternative mechanisms. Mol. Cell Biol. 2, 589-598 (2001).
76. LEITHAUSER, F., DHEIN, J., MECHTERSHEIMER, G., KORETZ, K., BRUDERLEIN, S., HENNE, C., SCHMIDT, A., DEBARTIN, K. M., KRAMMER, P. H., MOLLER, P.: Constitutive and induced expression of APO-1, a new member of the nerve groth factor/tumor necrosis factor receptor superfamily, in normal and neoplastic cells. - Lab. Invest. 69, 415-429 (1993).
77. LESAULT, A., CASTELLANOS, A., WESTON, K.: c-Myb transcription is activated by protein kinase B (PKB) following interleukin 2 stimulation of T cells and is required for PKB-mediated protection from apoptosis. - Mol. Cell. Biol. 21, 5797-5805 (2001).
78. LESAULT, I., QUANG, C. T., FRAMPTON, J., GHYSDAE, L J.: Direct regulation of Bcl-2 by FLI-1 is involved in the survival of FLI-1-transformed erythroblasts. - EMBO J. 21, 694-703 (2002).
79. LI, P., NIJHAWAN, D., BUDIHARDJO, I., SRINIVASULA, L., AHMAD, S. M., ALNEMRI, E. S.: Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic cascade. - Cell 91, 479-489 (1997).
80. LOCKSHIN, R. A., WILLIAMS, C. M.: Programmed cell death. II. Endocrine potentiation of the breakdown of the intersegmental muscles of silkmoths. - J. Insect. Physiol. 10, 643-649 (1964).
81. LOCKSLEY, M. R., KILLEEN, N., LENARD, L. M.: The TNF and TNF receptor superfamilies: integrating mammalian biology. - Cell 104, 487-501 (2001).
82. LOS, M., VAN DE CRAEN, M., PENNING, L. C., SCHENK, H., WESTENDROP, M., BAEUERLE, P.A., DROGE, W., KRAMMER, P. H., FIERS, W., SCHULZ-OSTHOFF, K.: Requirement of an ICE/CED3 protease for Fas/APO-1-mediated apoptosis. - Nature 375, 81-83 (1995).
83. MAJNO, G., JORIS, I.: Apoptosis, oncosis, and necrosis. An overview of cell death. - Am. J. Pathol. 146, 3-15 (1995).
84. MARIANI, S. M., MATIBA, B., BAMLER, C., KRAMMEN, P. H.: Regulation of cell surface APO-1/Fas (CD95) ligand expression by metalloproteases. - Eur. J. Immunol. 25, 2303-2307 (1995).
85. MARTIN, S. J., REULTELINGSPERGER, C. P. M., MCGAHON, A. J., RADER, J. A., VAN SCHIE, R. C. A. A., LAFACE, D. M., GREEN, D. R.: Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: Inhibition by overexpression of Bcl-2 and Abl. - J. Exp. Med. 182, 1545-1556 (1995).
86. MAYO, M. W., WANG, C. Y., DROUIN, S. S., MADRID, L. V., MARSHALL, A. F., REED, J.C., WISSMAN, B. E., BALDWIN, A. S.: WT1 modulates apoptosis by transcriptionally upregulating the bcl-2 proto-oncogene. - EMBO J. 18, 3990-4003 (1999).
87. MIGHELI, A., ATTANASIO, A., SHIFFER, D.: Ultrastructural detection of DNA strand breaks in apoptotic neural cells by in situ end-labelling techniques. - J. Pathol. 176, 27-35 (1995).
88. MIYASHITA, T., HARIGAI, M., HANADA, M., REED, J. C.: Identification of a p53-dependent negative response element in the bcl-2 gene. - Cancer Res. 54, 3131-3135 (1994a).
89. MIYASHITA, T., KRAJEWSKI, S., KRAJEWSKA, M., WANG, H. G., LIN, H. K., LIEBERMANN, D. A., HOFFMAN, B., REED, J. C.: Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo. - Oncogene 9, 1799-1805 (1994b).
90. MIYASHITA, T., REED, J.: Tumor suppressor p53 is a regulator of the human bax gene. - Cell 80, 293-299 (1995).
91. MOOLTEN, I.: Drug sensitivity ("suicide") gene for selective cancer gene therapy. - Cancer Gene Ther. 1, 107-112 (1994).
92. MUNDLE, S., GAO, X. Z., KHAN, S., GREGORY, S. A., PRESLER, H. D., RAZA, A.: Two in situ labeling techniques reveal different patterns of DNA fragmentation during spontaneous apoptosis in vivo and induced apoptosis in vitro. - Anticancer Res. 15, 1895-1904 (1995).
93. NAGATA, S., GOLSTEIN, P.: The Fas death factor. - Science 267, 1449-1456 (1995).
94. NAKAMURA, K., ITO, Y., MATSUMOTO, K., DAIKOKU, E., KIYOKANE, K., OTSUKI, Y.: The relationship between apoptosis and keratinization in human epidermis. - Acta. Histochem. Cytoc. 32, 77-83 (1999).
95. NAKAMURA, M., YAGI, H., ISHII, T., KAYABE, S., SOGA, H., GOTOH, S., OGATA, M., ITOH, T.: DNA fragmentation is not the primary event in glucocorticoid-induced thymocyte death in vivo. Eur. J. Immunol. 27, 999-1004 (1997).
96. NAKAMURA, M., YAGI, H., KAYABE, S., ISHII, T., OHTSU, S., GOTOH, T., ITOH, T.: Most thymocytes die in the absence of DNA fragmentation. - Arch. Histol. Cytol. 58, 249-256 (1995).
97. NAKAO, K., OTSUKI, Y., AKAO, Y., ITO, Y., MARUKAWA, O., TACHIBANA, S., KAWAKAMI, M., SASAKI, S.: The synergistic effects of hyperthermia and anticancer drugs on induction of apoptosis. - Med. Electron. Microsc. 33, 44-50 (2000).
98. NICHOLSON, D. W, ALI, A., THORNBERRY, N. A., VAILLANCOURT, J. P., DING, C. K., GALLANT, M., GAREAU, Y., GRIFFIN, P. R., LABELLE, M., LAZEBNIK, Y. A.: Identification and inhibi tion of the ICE/CED-3 protease necessary for mammalian apoptosis. - Nature 376, 37-43 (1995).
99. NICOTERA, P., LEIST, M.: Energy supply and the shape of death in neurons and lymphoid cells. Cell Death Differ. 4, 435-442 (1997).
100. NISHIMURA, Y., HIRABAYASHI, Y., MATSUZAKI, Y., MUSETTE, P., ISHII, A., NAKAUCHI, H., INOUE, T., YONEHARA, S.: In vivo analysis of Fas antigen-mediated apoptosis: effects of agonistic antimouse Fas mAb on thymus, spleen and liver. - Int. Immunol. 9, 307-316 (1977).
101. NISHIMURA, S., ADACHI, M., ISHIDA, T., MATSUNAGA, T., UCHIDA, H., HAMADA, H., IMAI, K.: Adenovirus-mediated transfection of caspase-8 augments anoikis and inhibits peritoneal dissemination of human gastric carcinoma cells. - Cancer Res. 61, 7009-7014 (2001).
102. NO, D., YAO, T. P., EVANS, R. M.: Ecdysone-inducible gene expression in mammalian cells and transgenic mice. - Proc. Natl. Acad. Sci., USA 93, 3346-3351 (1996).
103. O'CONNELL, J., O'SULLIVAN, G. C., COLLINS, J. K., SHANAHAN, F.: The Fas counterattack: Fasmediated T cell killing by colon cancer cells expressing Fas ligand. - J. Exp. Med. 184, 1075-1082 (1996).
104. OLTAVI, Z., MILLMANN, C., KORSMEYER, S.: Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. - Cell 74, 609-619 (1993).
105. ORTH, K., DIXIT, V. M.: Bik and Bax induce apoptosis downstream of CrmA but upstream of inhibitor of apoptosis. - J. Biol. Chem. 272, 8841-8844 (1997).
106. OTSUKI, Y.: Various methods of apoptosis detection. - Acta Histochem. Cytochem. 33, 235-241 (2000).
107. OTSUKI, Y., MISAKI, O., SUGIMOTO, O., ITO, Y., TSUJIMOTO, Y., AKAO, Y.: Cyclic bcl-2 gene expression in human uterine endometrium during menstrual cycle. - Lancet 344, 28-29 (1994).
108. PARDO, O. E., ARCARO, A., SALERNO, G., RAGUZ, S., DOWNWARD, J., SECKL, M. J.: Fibroblast growth factor-2 induces translational regulation of Bcl-XL and Bcl-2 via a MEK-dependent pathway: correlation with resistance to etoposide-induced apoptosis. - J. Biol. Chem. 277, 12040-12046 (2002).
109. PARK, H. J.: Effects of intracellular pH on apoptosis in HL-60 human leukemia cells. - Yonsei Med. J. 36, 473-479 (1995).
110. PATTOROSSI, A., BATTAGLIA, A., MAGGIANO, A., MALINCONICO, P., ANDREOCCI, L., MANCUSO, S., SCAMBIA, G.: Laser scanning cytometry can complement the flow cytometric DNA analysis in paraffin-embedded cancer samples: a paradigmatic case. - Cytometer 42, 123-125 (2000).
111. POTTEN, C. S.: The significance of spontaneous and induced apoptosis in the gastrointestinal tract of mice. - Cancer Metastasis Rev. 11, 179-195 (1992).
112. POWELL, L. M., WAILLIS, S. C., PEASE, R. J., EDWARDS, Y. H., KNOTTT, T. J., SCOTT, J.: A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine. - Cell 50, 831-840 (1987).
113. PUGAZHENTHI, S., MILLER, E., SABLE, C., YOUNG, P., HEIDENREICH, K. A., BOXER, L. M., REUSCH, J. E.: Insulin-like growth factor-I induces bcl-2 promoter through the transcription factor cAMP-response element-binding protein. - J. Biol. Chem. 274, 27529-27535 (1999).
114. PUGAZHENTHI, S., NESTEROVA, A., SABLE, C., HEIDENREICH, K. A., BOXER, L. M., HEASLEY, L. E., REUSCH, J. E.: Akt/protein kinase B up-regulates Bcl-2 expression through cAMP-response element-binding protein. - J. Biol. Chem. 275, 10761-10766 (2000).
115. QIN, J. Z., ZHANG, C. L., KAMARASHEV, J., DUMMER, R., BRUG, G., DOBBELING, U.: Interleukin7 and interleukin-15 regulate the expression of the bcl-2 and c-myb genes in cutaneous T-cell lymphoma cells. - Blood 98, 2778-2783 (2001).
116. RAO, R. V., HERMEL, E., CASTRO-OBREGON, S., DEL RIO, G., ELLERBY, L. M., ELLERBY, H. M., BREDESEN, D. E.: Coupling endoplasmic reticulum stress to the cell death program. - J. Biol. Chem. 276, 33869-33874 (2001).
117. RECKLINGHAUSEN, F., von: Untersuchungen uber Rachitis und Osteomalacie. - Verlag Gustav Fischer, Jena (1910).
118. REED, J. C.: Bcl-2 and the regulation of programmed cell death. - J. Cell Biol. 124, 1-6 (1994).
119. ROMERO, F., MARTINEZ, A. C., CAMMONIS, J., REBOLLO, A.: Aiolos transcription factor controls cell death in T cells by regulating Bcl-2 expression and its cellular localization. - EMBO J. 18, 3419-3430 (1999).
120. ROTHBARTH, P. H., HENDRIKS-STURKENBOOM, I., PLOEM, J. S.: Identification of monocytes in suspensions of mononuclear cells. - Blood 48, 139-147 (1976).
121. SAHARA, S., AOTA, M., EGUCHI, Y., IMAMOTO, N., YONEDA, Y., TSUJIMOTO, Y.: Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. - Nature 401, 168-172 (1999).
122. SALOMONI, P., PERROTTI, D., MARTINEZ, R., FRANCESCHI, C., CALABRETTA, B.: Resistance to apoptosis in CTLL-2 cells constitutively expressing c-Myb is associated with induction of BCL-2 expression and Myb-dependent regulation of bcl-2 promoter activity. - Proc Natl. Acad. Sci. USA 94, 3296-3301 (1997).
123. SALZMAN, G. C., SINGHAM, S. B., JOHNSTON, R. G., BOHREN, C. F.: Light scattering and cytometry. In: Flow Cytometry and Sorting.-Wiley-Liss, New York (1990).
124. SAUNDERS, J. W., FALLON, J. F.: Cell death in morphogenesis. - In: Major problems in developmental biology, pp.289-314. - Academic Press, New York (1967).
125. SELLNER, L. N., COELEN, R. J., MACKENZIE, J. S.: Reverse transcriptase inhibits Taq polymerase activity. - Nucleic Acid Res. 20, 1487-1490 (1992).
126. SETO, M., JAEGER, U., HOCKETT, R. D., GRANINGER, W., BENNETT, S., GOLDMAN, P., KORSMEYER, S. J.: Alternative promoters and exons, somatic mutation and deregulation of the Bcl-2-Ig fusion gene in lymphoma. - EMBO J. 7, 123-131 (1988).
127. SCOPSI, L., LARSSON, L. I., BASTHOLM, L., NIELSEN, M. M.: Silver-enhanced colloidal gold probes as markers for scanning electron microscopy. - Histochemistry 86, 35-41 (1986).
128. SHERWOOD, S. W., SCHIMKE, R. T.: Cell cycle analysis of apoptosis using flow cytometry. - Methods Cell Biol. 46, 77-97 (1995).
129. SHIMUZU, S., EGUCHI, Y., ITO, Y., HASEWAGA, J., YAMABE, K., OTSUKI, Y., MATSUDA, H., TSUJIMOTO, Y.: Induction of apoptosis as well as necrosis by hypoxia and predominant prevention of apoptosis by Bcl-2 and Bcl-XL. - Cancer Res. 56, 2161-2166 (1996).
130. SHIBATA, M-A., LIU, M.-L., KNUDSON, M. C., SHIBATA, E., YOSHIDOME, K., BANDY, T., KORSMEYER, S. J., GREEN, J. E.: Haploid loss of bax leads tp accelerated mammary tumor development in C3(1)/SV40-TAg transgenic mice: reduction in protective apoptotic response at the preneoplastic stage. - EMBO J. 18, 2692-2701 (1999).
131. SHIBATA, M-A., MORIMOTO, J., OTSUKI, Y.: Suppression of murine mammary carcinoma growth and metastasis by HSVtk/GCV gene therapy using in vivo electroporation. - Cancer Gene Ther. 9, 16-27 (2002).
132. SHIBATA, M-A., YOSHIDOME, K., SHIBATA, E., JORYCK, C. L., GREEN, J. E.: Suppression of mammary carcinoma growth in vitro and in vivo by inducible expression of the Cdk inhibitor p21. - Cancer Gene Ther. 8, 23-35 (2001).
133. SHIOKAWA, D., OHYAMA, H., YAMADA, T., TAKAHASHI, K., TANUMA, S.: Identification of an endonuclease responsible for apoptosis in rat thymocytes. - Eur. J. Biochem. 226, 23-30 (1994).
134. SMITH, D., SIEG, S., KAPLAN, D.: Technical Note: Aberrant detection of cell surface Fas ligand with anti-peptide antibodies. -J. Immunology 160, 4159-4160 (1998).
135. SMITH, M. D., ENSOR, E. A., COFFIN, R. S., BOXER, L. M., LATCHMAN, D. S.: Bcl-2 transcription from the proximal P2 promoter is activated in neuronal cells by the Brn-3a POU family transcription factor. - J. Biol. Chem. 273, 16715-16722 (1998).
136. SMITH, M. D., MELTON, L. A., ENSOR, E. A., PACKHAM, G., ANDERSON, P., KINLOCH, R. A., LATCHMAN, D. S.: Brn-3a activates the expression of Bcl-x(L) and promotes neuronal survival in vivo as well as in vitro. - Mol. Cell. Neurosci. 17, 460-470 (2001).
137. SUDA, T., OKAZAKI, T., NAITO, Y., YOKOTA, T., ARAI, N., OZAKI, S., NAKAO, K., NAGATA, S.: Expression of the Fas ligand in cells of T cell lineage. - J. Immunol. 154, 3806-3813 (1995).
138. SUZUKI, H., FUKUYAMA, K., EPSTEIN, W.L.: Changes in nuclear DNA and RNA during epidermal keratinization. - Cell Tissue Res. 184, 155-167 (1977)
139. TAMATANI, M., CHE, Y. H., MATSUZAKI, H., OGAWA, S., OKADA, H., MIYAKEE, S., MIZUNO, T., TOYAHAMA, M.: Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappaB activation in primary hippocampal neurons. -J. Biol. Chem. 274, 8531-8538 (1999).
140. TANAKA, M., SUDA, T., HAZE, K., NAKAMURA, N., SATO, K., KIMURA, F., MOTOYOSHI, K., MIZUKI, M., TAGAWA, S., OHGA, S., HATAKE, K., DRUMMOND, A. H., NAGATA, S.: Fas ligand in human serum. - Nature Med. 2, 317-322 (1996).
141. TANUMA, S., SHIOKAWA, D.: Multiple forms of nuclear deoxyribonuclease in rat thymocytes. Biochem. Bioph. Res. Cmm. 203, 789-797 (1994).
142. TAYLOR, D., BADIANI, P., WESTON, K.: A dominant interfering Myb mutant causes apoptosis in T cells. - Genes Dev. 10, 2732-2744 (1996)
143. TEWARI, M., QUAN, L. T., O'ROURKE, K., DESNOYERS, S., ZENG, Z., BEIDLER, D. R., POIRIER, G. G., SALVESEN, G. S., DIXIT, V. M.: Yama/CPP32b, a mammalian homolog of CED-3, is a crmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose)polymerase. - Cell 81, 801-809 (1995).
144. THIRY, M.: In situ nick translation at the electron microscopic level: a tool for studying the location of DNase I-sensitive regions within the cell. - J. Histochem. Cytochem. 39, 871-874 (1991).
145. TILLY, J. L., HSUEH, J. W.: A microscale autoradiographic analysis of apoptotic DNA fragmentation. -J. Cell. Physiol. 131, 1063-1068 (1992).
146. TSUJIMOTO, Y., CROCE, C. M.: Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. - Proc. Natl. Acad. Sci. USA 83, 5214-5218 (1986).
147. TSUJIMOTO, Y.: Stress-resistance conferred by high level of bcl-2 αprotein in human B lymphoblastoid cell. - Oncogene 4, 1331-1336 (1989).
148. UMEMURA, S., KAWAI, K., OSAMURA, Y., TSUTSUMI, Y.: Antigen retrieval for bcl-2 in formalin-fixed, paraffin-embedded sections. - Pathol. Int. 45, 103-107 (1995).
149. VANDER-HEIDEN, M. G., CHANDEL, N. S., WILLIAMSON, E. K., SCHUMACKER, P. T., THOMPSON, C. B.: Bcl-XL regulates the membrane potential and volume homeostasis of mitochondria. Cell 91, 627-637 (1997).
150. VERDAGUER, E., PUBILL, D., RIMBAU, V., JIMENEZ, A., ESCUBEDO, E., CAMARASA, J., PALLAS, M., CAMINS, A.: Evaluation of neuronal cell death by laser scanning cytometry. - Brain Res. Protoc. 9, 41-48 (2002).
151. VERHEAGEN, S., MCGOWAN, A. J., BROPHY, A. R., FERNANDE, R. S., COTTER, T. G.: Inhibition of apoptosis by antioxidants in the human HL-60 leukemia cell line. - Biochem. Pharmacol. 50, 1021-1029 (1996).
152. VERMES, I., HAANEN, C., STEFFENS-NAKKE, H., REUTELINGSPERGER, C.: A novel assay for apoptosis flow cytometric detection of phosphatidylserine espression on early apoptotic cells using fluorescein-labeled annexin V. - Immunol. Methods 184, 39-51 (1995).
153. WANG, R.-F., CAO, W.-W., JOHNSON, M. G.: A simplified, single tube, single buffer system for RNA-PCR. - BioTechniques 12, 702-704 (1992).
154. WIJSMAN, J. H., JONKER, R. R., KEIJZER, R., VAN DE VELDE, C. J., CORNELISSE, C. J., VAN DIERENDONCK, J. H.: A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA. - J. Histochem. Cytochem. 41, 7-12 (1993).
155. XIANG, J., CHAO, D. T., KORSMEYER, S. J.: BAX-induced cell death may not require interleukin 1 beta-converting enzyme-like proteases. - Proc. Natl. Acd. Sci., USA 93, 14559-14563 (1996).
156. YAMASHITA, H., OTSUKI, Y., ITO, Y., MATSUMOTO, K., UEKI, K., UEKI, M.: Fas ligand, Fas antigen and Bcl-2 expression in human endometrium during the menstrual cycle. - Mol. Hum. Reprod. 5, 358-364 (1999).
157. YONEHARA, S.: Effects of anti-Fas antibodies on lymphocytes and other organs: preparation of original and new monoclonal antibodies and amelioration of systemic autoimmune disease. Int. Rev. Immunol. 18, 329-345 (1999).
158. ZHANG, G., GURTU, V., KAIN, S. R., YAN, G.: Early detection of apoptosis using a fluorescense conjugate of annexin V. - BioTechniques 23, 525-531 (1997).
159. ZHANG, G., GURTU, V., SPENCER, C., MA, J.-T., KAIN, S.: Detection of caspase activity associated with apoptosis using fluorometric and colorimetric methods. In: Apoptosis Detection and Assay Methods.-Eaton Publishing, Natick, MA (USA) 1998.
160. ZHOU, Z., SUN, X., KANG, X. J.: Ethanol-induced apoptosis in mouse liver: Fas- and cytochrome c-mediated caspase-3 activation pathway. - Am. J. Pathol. 159, 329-338 (2001).