Early detection of apoptosis using a fluorescent conjugate of annexin V

BioTechniques

Volumn 23, Issue 3, 1997, Pages 525-531

  Zhang, Guohong ^a   Gurtu, Vanessa ^a   Kain, Steven R ^a   Yan, Guochen ^b  

^a CLONTECH Laboratories Inc   (United States)

^b SUGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLOHEXIMIDE; DACTINOMYCIN; DNA FRAGMENT; ETOPOSIDE; FLUORESCEIN ISOTHIOCYANATE; LIPOCORTIN 5; PHOSPHATIDYLSERINE; STAUROSPORINE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN;

ANIMALIA; MAMMALIA;

EID: 0030883765     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97233pf01     Document Type: Article

References (32)

1. Andree, H.A.M., C.P.M. Reutelingsperger, R. Hauptmann, H.C. Hemker, W.T. Hermens and G.M. Willems. 1990. Binding of vascular anticoagulant a (VACa) to planar phospholipid bilayers. J. Biol. Chem. 265:4923-4928.
2. Arends, M.J. and A.H. Wyllie. 1991. Apoptosis: mechanism and roles in pathology. Int. Rev. Exp. Pathol. 32:223-254.
3. Baffy, G., T. Miyashita, J.R. Williamson and J.C. Reed. 1993. Apoptosis induced by withdrawal of interleukin-3 (IL-3) from an IL-3-dependent hematopoietic cell line is associated with repartitioning of intracellular calcium and is blocked by enforced Bcl-2 oncoprotein production. J. Biol. Chem. 268:6511-6519.
4. Cohen, J.J., R.C. Duke, V.A. Fadok and K.S. Sellins. 1992. Apoptosis and programmed cell death in immunity. Annu. Rev. Immunol. 10:267-293.
5. Duvall, E., A.H. Wyllie and R.G. Morris. 1985. Microphage recognition of cells undergoing programmed cell death (apoptosis). Immunology 56:351-358.
6. Facchinetti, A., L. Tessarollo, M. Mazzocchi, R. Kingston, D. Collavo and G. Biasi. 1991. An improved method for the detection of DNA fragmentation. J. Immunol. Methods 136:1251-1256.
7. Fadok, V.A., D.J. Laszlo, P.W. Nobel, L. Weinstein, D.W.H. Riches and P.M.J. Henson. 1993. Particle digestibility is required for induction of the phosphatidylserine recognition mechanism used by murine macrophages to phagocytose apoptotic cells. J. Immunol. 151:4274-4285.
8. Fadok, V.A., J.S. Savill, C. Haslett, D.L. Bratton, D.E. Doherty, P.A. Campbell and P.M. Henson. 1992. Different populations of macrophages use either the vitronectin receptor or the phosphatidylserine receptor to recognize and remove apoptotic cells. J. Immunol. 149:4029-4035.
9. Fadok, V.A., D. Voelker, P.A. Campbell, J.J. Cohen, D.L. Bratton and P.M. Henson. 1992. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by microphages. J. Immunol. 148:2207-2216.
10. Homburg, C.H.E., M. de Haas, A.E.G.Kr. von dem Borne, A.J. Verhoeven, C.P.M. Reutelingsperger and D. Roos. 1995. Human neutrophils lose their surface FcγRIII and acquire annexin V binding sites during apoptosis in vitro. Blood 85:532-540.
11. Koopman, G., C.P.M. Reutelingsperger, G.A.M. Kuijten, R.M.J. Keehnen, S.T. Pals and M.H.J. van Oers. 1994. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84:1415-1420.
12. Martin, S.J. and D.R. Green. 1995. Apoptosis and cancer: the failure of controls on cell death and cell survival. Crit. Rev. Oncol./Hematol. 18:137-153.
13. Martin, S.J., C.P.M. Reutelingsperger, A.J. McGahon, J.A. Rader, R.C.A.A. van Schie, D.M. LaFace and D.R. Green. 1995. Earlier distribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J. Exp. Med. 182:1545-1556.
14. McConkey, D.J. and S. Orrenius. 1995. Calcium and cyclosporine A in the regulation of apoptosis. Curr. Top. Microbiol. Immunol. 200:95-105.
15. Morris, R.G., A.D. Hargreaves, E. Duval and A.H. Wyllie. 1984. Hormone-induced death. II. Surface changes in thymocytes undergoing apoptosis. Am. J. Pathol. 115:426-431.
16. Newman, S.L., J.E. Henson and P.M. Henson. 1982. Phagocytosis of senescent neutrophils by human monocyte-derived macrophages and rabbit inflammatory macrophages. J. Exp. Med. 156:430-442.
17. Nicoletti, I., G. Migliorati, M.C. Pagliacci, F. Grignani and C. Riccardi. 1991. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometer. J. Immunol. Methods 139:271-276.
18. Op den Kamp, J.A.F. 1979. Lipid asymmetry in membranes. Annun. Rev. Biochem. 48:47-68.
19. Ormerod, M.G., X.M. Sun, D. Brown, R.T. Snowden and G.M. Cohen. 1993. Quantification of apoptosis and necrosis by flow cytometry. Acta Oncol. 32:417-423.
20. Pytela, R., M.D. Pierschbaccher and E.A. Ruoslahti. 1985. A 125/115 kDa cell surface receptor specific for vitronectin interacts with the Arg-Gly-Asp adhesion sequence derived from fibronectin. Proc. Natl. Acad. Sci. USA 82:5766-5770.
21. Reutelingsperger, C.P.M., G. Hornstra and H.C. Hemker. 1985. Isolation and partial purification of a novel anticoagulant from arteries of human umbilical cord. Eur. J. Biochem. 151:625-629.
22. Sarraf, C.E. and I.D. Bowen. 1988. Proportions of mitotic and apoptotic cells in a range of untreated experimental tumors. Cell Tissue Kinet. 21:45-49.
23. Savill, J.S., V. Fadok, P.M. Henson and C. Haslett. 1993. Phagocytic recognition of cells undergoing apoptosis. Immunol. Today 14:131-136.
24. Savill, J.S., P.M. Henson and C. Haslett. 1989. Phagocytosis of aged human neutrophils by macrophages is mediated by a novel "charge-sensitive" recognition mechanism. J. Clin. Invest. 84:1518-1527.
25. Schmid, I., C.H. Uittenbogaart and J.V. Giorgi. 1994. Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry. Cytometry 15:12-17.
26. Tait, J.F., D. Gibson and K. Fujikawa. 1989. Phospholipid binding properties of human placental anticoagulant protein-I, a member of the lipocortin family. J. Biol. Chem. 264:7944-7949.
27. Telford, E.G., L.E. King and P.J. Fraker. 1991. Evaluation of glucocorticoid-induced DNA fragmentation in mouse thymocytes by flow cytometry. Cell Prolif. 24:447-459.
28. Telford, E.G., L.E. King and P.J. Fraker. 1992. Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13:137-142.
29. Thiagarajan, P. and J.F. Tait. 1990. Binding of annexin V/placental anticoagulant protein I to platelets. Evidence for phosphatidylserine exposure in the procoagulant response of activated platelets. J. Biol. Chem. 265:17420-17423.
30. Vermes, I., C. Haanen, H. Steffens-Nakken and C. Reutelingsperger. 1995. A novel assay for apoptosis flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled annexin V. J. Immunol. Methods 184:39-51.
31. Wijsman, J.H., R.R. Jonker, R. Keijzer, C.J.H. Van de Velde, C.J. Cornelisse and J.H. Van Dierendonck. 1993. A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA. J. Histochem. Cytochem. 41:7-12.
32. Wyllie, A.H., J.F.R. Kerr and A.R. Currie. 1980. Cell death: the significance of apoptosis. Int. Rev. Cytol. 68:251-306.