Quantitative RT-PCR using a PCR-generated competitive internal standard

BioTechniques

Volumn 23, Issue 1, 1997, Pages 34-36

  Fille, Manfred ^a   Shanley, John D ^a   Aslanzadeh, Jaber ^a,b  

^a UNIVERSITY OF CONNECTICUT   (United States)

^b UNIVERSITY OF CONNECTICUT SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; HEPATITIS C; HUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA SYNTHESIS;

HEPATITIS C VIRUS;

EID: 0030758529     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97231bm05     Document Type: Article

References (5)

1. Aslanzadeh, J., B.B. Padilla and J.D. Shanley. 1996. Evaluation of PCR and nested PCR for laboratory diagnosis of hepatitis C virus infection. Mol. Cell. Probes 10:173-178.
2. Clossais Besnard, N. and P.M. Andre. 1994. Automated quantitative determination of hepatitis C virus viremia by reverse transcription-PCR. J. Clin. Microbiol. 32:1887-1893.
3. Gilliland, G., S. Perrin, K. Blanchard and H.F. Bunn. 1990. Analysis of cytokine mRNA and DNA: detection and quantification by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87:2725-2729.
4. Lau, Y.N.J., G.L. Davis, J. Kniffen, K.-P. Qian, M.S. Urdea, C.S. Chan, M. Mizokami, P.D. Neuwald and J.C. Wilber. 1993. Significance of serum hepatitis C virus RNA levels in chronic hepatitis C. Lancet 341: 1501-1504.
5. Manzin, A., P. Bagnarelli, S. Menzo, F. Giostra, M. Brugia, R. Francesconi, F.B. Bianchi and M. Clementi. 1994. Quantitation of hepatitis C virus genome molecules in plasma samples. J. Clin. Microbiol. 32:1939-1944.