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Volumn 15, Issue 2, 2003, Pages 182-189

Antigen receptor selection by editing or downregulation of V(D)J recombination

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; CD3 ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; LYMPHOCYTE ANTIGEN RECEPTOR;

EID: 0037375554     PISSN: 09527915     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0952-7915(03)00008-6     Document Type: Review
Times cited : (65)

References (89)
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    • William J., Euler C., Christensen S., Shlomchik M.J. Evolution of autoantibody responses via somatic hypermutation outside of germinal centers. Science. 297:2002;2066-2070 Autoreactive B cells in IgH transgenic MRL/lpr mice were analyzed by splenic sectioning, microdissection, PCR amplification and L-chain gene sequencing to determine if extrafollicular diversification occurred. The authors found evidence for V gene mutation in cells accumulating at the T cell/ red pulp border, suggesting that hypermutation can occur outside of germinal centers.
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    • Magari M., Sawatari T., Kawano Y., Cascalho M., Wabl M., Kanayama N., Hikida M., Ohmori H. Contribution of light chain rearrangement in peripheral B cells to the generation of high-affinity antibodies. Eur. J. Immunol. 32:2002;957-966 Taking advantage of their observation that RAG expression in peripheral B cells is IL-7 dependent, these investigators tested the effect of anti-IL-7 receptor treatment on affinity maturation and κ/λ usage among antibodies stimulated by immunization of a IgH-chain knock-in mouse with a hapten-protein conjugate. The findings indicate that blocking receptor revision in vivo has a major impact on affinity maturation and suppresses the extent of Igλ antibody production.
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    • H regions tested the notion that the VJκ joins that they often contain were edited after the onset of somatic mutation. Of 265 cells tested, none showed evidence that could unequivocally support the hypothesis, suggesting either that revision made a negligible contribution to diversity or did so only before the onset of somatic mutation
    • H regions tested the notion that the VJκ joins that they often contain were edited after the onset of somatic mutation. Of 265 cells tested, none showed evidence that could unequivocally support the hypothesis, suggesting either that revision made a negligible contribution to diversity or did so only before the onset of somatic mutation.
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    • The tight interallelic positional coincidence that distinguishes T-cell receptor Jα usage does not result from homologous chromosomal pairing during VαJα rearrangement
    • This study analyzed the recombination status of both TCRα chromosomes in a large number of T cell clones. In almost all clones, rearrangement to a similarly positioned J region occurred on both chromosomes. Fluorescence in situ hybridization (FISH) analysis showed that this positional coincidence could not be explained by the physical juxtaposition of homologous Jα regions. Although a trend was seen for V regions, the positional coincidence was not as tight as for J regions. In-frame rearrangements were found on both alleles in approximately 1/3 clones
    • Davodeau F., Difilippantonio M., Roldan E., Malissen M., Casanova J.L., Couedel C., Morcet J.F., Merkenschlager M., Nussenzweig A., Bonneville M., Malissen B. The tight interallelic positional coincidence that distinguishes T-cell receptor Jα usage does not result from homologous chromosomal pairing during VαJα rearrangement. EMBO J. 20:2001;4717-4729 This study analyzed the recombination status of both TCRα chromosomes in a large number of T cell clones. In almost all clones, rearrangement to a similarly positioned J region occurred on both chromosomes. Fluorescence in situ hybridization (FISH) analysis showed that this positional coincidence could not be explained by the physical juxtaposition of homologous Jα regions. Although a trend was seen for V regions, the positional coincidence was not as tight as for J regions. In-frame rearrangements were found on both alleles in approximately 1/3 clones.
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    • This paper provides a comprehensive analysis of V-J joining frequency in mouse thymocytes. The data support a bi-directional and coordinated model of recombination beginning with the most 3′ V regions and 5′ J regions. The paper also showed that the TCR V and J regions become gradually accessible from day 18 in gestation until one day after birth
    • Pasqual N., Gallagher M., Aude-Garcia C., Loiodice M., Thuderoz F., Demongeot J., Ceredig R., Marche P.N., Jouvin-Marche E. Quantitative and qualitative changes in V-J alpha rearrangements during mouse thymocytes differentiation: implication for a limited T cell receptor alpha chain repertoire. J. Exp. Med. 196:2002;1163-1174 This paper provides a comprehensive analysis of V-J joining frequency in mouse thymocytes. The data support a bi-directional and coordinated model of recombination beginning with the most 3′ V regions and 5′ J regions. The paper also showed that the TCR V and J regions become gradually accessible from day 18 in gestation until one day after birth.
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    • + T cells upon superantigen encounter. Because the antigen was introduced, the authors could show that revision takes approximately three weeks to become maximal.
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