Down-regulation of PIK3CG, a catalytic subunit of phosphatidylinositol 3-OH kinase, by CpG hypermethylation in human colorectal carcinoma

Clinical Cancer Research

Volumn 8, Issue 12, 2002, Pages 3824-3831

  Semba, Shuho ^a   Itoh, Nanami ^a   Ito, Masafumi ^a   Youssef, Emile M ^b   Harada, Masaru ^a   Moriya, Toshiyuki ^a   Kimura, Wataru ^a   Yamakawa, Mitsunori ^a  

^a YAMAGATA UNIVERSITY   (Japan)

^b UNIVERSITY OF TEXAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 MORPHOLINO 8 PHENYLCHROMONE; 5 AZA 2' DEOXYCYTIDINE; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B;

ARTICLE; CANCER CELL; CANCER GROWTH; CANCER INVASION; CARCINOGENESIS; CATALYSIS; CELL LINE; CELL PROLIFERATION; COLORECTAL CARCINOMA; CONTROLLED STUDY; DOWN REGULATION; DRUG EFFECT; GENE; GENE OVEREXPRESSION; GENE SILENCING; GROWTH INHIBITION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; METASTASIS; METHYLATION; PIK3CG GENE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ADULT; AGED; AGED, 80 AND OVER; AZACITIDINE; CHROMONES; CHROMOSOMES, HUMAN, PAIR 7; COLORECTAL NEOPLASMS; CPG ISLANDS; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; DNA METHYLATION; DNA, NEOPLASM; DOWN-REGULATION; ENZYME INHIBITORS; FEMALE; HUMANS; MALE; MIDDLE AGED; MORPHOLINES; PHOSPHORYLATION; PROTEIN SUBUNITS; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-AKT; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, NEOPLASM; TUMOR CELLS, CULTURED;

EID: 0036898614     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Toker, A., and Cantley, L. C. Signalling through the lipid products of phosphoinositide 3-OH kinase. Nature (Lond.), 387: 673-676, 1997.
2. Chang, H. W., Aoki, M., Fruman, D., Auger, K. R., Bellacosa, A., Tsichlis, P. N., Cantley, L. C., Roberts, T. M., and Vogt, P. K. The retroviral oncogene p3k is homologous to the gene encoding for the catalytic subunit of phosphoinositide 3-kinase. Science (Wash. DC). 276: 1848-1850, 1997.
3. Franke, T. F., Kaplan, D. R., and Cantley, L. C. P13K: downstream AKTion blocks apoptosis. Cell, 88: 435-437, 1997.
4. Cantley, L. C., and Neel, B. G. New insight into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway. Proc. Natl. Acad. Sci. USA, 96: 4240-4245, 1999.
5. Itoh, N., Semba, S., Ito, M., Takeda, H., Kawata, S., and Yamakawa, M. Phosphorylation of Akt/PKB is required for suppression of cancer cell apoptosis and tumor progression in human colorectal carcinoma. Cancer (Phila.), in press, 2002.
6. Sakurada, A., Suzuki, A., Sato, M., Yamakawa, H., Orikasa, K., Uyeno, S., Ono, T., Ohuchi, N., Fujimura, S., and Horii, A. Infrequent genetic alterations of the PTEN/MMAC1 gene in Japanese patients with primary cancer of the breast, lung, pancreas, kidney, and ovary. Jpn. J. Cancer Res., 88: 1025-1028, 1997.
7. Stoyanov, B, Volinia, S., Hanck, T., Rubio, I., Loubtchenkov, M., Malek, D., Stoyanova, S., Vanhaesebroeck, B., Dhand, R., and Nurnberg, B. Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase. Science (Wash. DC), 269: 690-693, 1995.
8. Stephens, L. R., Eguinoa, A., Erdjument-Bromage, H., Lui, M., Cooke, F., Coadwell, J., Smrcka, A. S., Thelen. M., Cadwallader, K., Tempst, P., and Hawkins, P. T. The G βγ sensitivity of a PI3K is dependent upon a tightly associated adoptor, p101. Cell, 89: 105-114, 1997.
9. Sasaki. T., Irie-Sasaki, J., Jones, R. G., Oliveira-dos-Santos, A. J., Stanford, W. L., Bolon, B., Wakeham, A., Itie, A., Bouchard, D., Kozieradzki, I., Joza, N., Mak, T. W., Ohashi, P. S., Suzuki, A., and Penninger, J. M. Function of PI3Kγ in thymocyte development. T cell activation, and neutrophil migration. Science (Wash. DC), 287: 1040-1046, 2000.
10. Li, Z., Jiang, H., Xie, W., Zhang, Z., Smrcka, A. V., and Wu, D. Roles of PLC-β2 and β3 and PI3K in chemoattractant-mediated signal transduction. Science (Wash. DC), 287: 1046-1053, 2000.
11. Sasaki, T., Irie-Sasaki, J., Horie, Y., Bachmaier, K., Fata, J. E., Ki, M., Suzuki, A., Bouchard, D., Ho, A., Redson, M., Gallinger, S., Khokha. R., Mark, T. W., Hawkins, P. T., Stephens, L., Scherer, S. W., Tsao. M., and Penninger, M. Colorectal carcinomas in mice lacking the catalytic subunit of PI(3)Kγ. Nature (Lond.), 406: 897-902, 2000.
12. Fearon, E. R., and Vogelstein, B. A genetic model for colorectal tumorigenesis. Cell, 61: 759-767, 1990.
13. Japanese Society for Cancer of Colon and Rectum. The Japanese Criteria of General Rules for Clinical and Pathological Studies or Cancer of the Colon, Rectum and Anus. Ed. 6. Tokyo: Kanehara & Co. Ltd., 1998.
14. Semba, S., Itoh, N., Ito, M., Harada, M., and Yamakawa, M. The in vitro and in vivo effects of 2-(4-morpholinyl)-8-phenylchromone (LY294002), a specific inhibitor of phosphatidylinositol 3′ kinase, in human colon cancer cells. Clin Cancer Res., 8: 1957-1963, 2002.
15. Vlahos, C. J., Matter, W. F., Hui, K. Y., and Brown, R. F. A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J. Biol. Chem., 269: 5241-5218, 1994.
16. Sano, T., Tsujino, T., Yoshida, K., Nakayama, H., Haruma, K., Ito, H., Nakayama, Y., Kajiyama, G., and Tahara, E. Frequent loss of heterozygosity on chromosome 1q, 5q, and 17p in human gastric cancer. Cancer Res., 51: 2926-2931, 1991.
17. Sato, S., Yokozaki, H., Yasui, W., Nikai, H., and Tahara, E. Silencing of the CD44 gene by CpG methylation in a human gastric cancer cell line. Jpn. J. Cancer Res., 90: 485-489, 1999.
18. Semba, S., Yokozaki, H., Yasui, W., and Tahara, E. Frequent microsatellite instability and loss of heterozygosity in the region including BRCA1 (17q21) in young patients with gastric cancer. Int. J. Oncol., 12: 1245-1251, 1998.
19. Yasui, W., Kudo, Y., Semba, S., Yokozaki, H., and Tahara, E. Reduced expression of cyclin-dependent kinase inhibitor p27KIP1 is associated with advanced stage and invasiveness of gastric carcinomas. Jpn. J. Cancer Res., 88: 625-629, 1997.
20. Semba, S., Han, S. Y., Ikeda, H., and Horii, A. Frequent nuclear accumulation of β-catenin in pituitary adenoma. Cancer (Phila.), 91: 42-48, 2001.
21. Aoki, M., Hecht, A., Kruse, U., Kemler, R., and Vogt, P. K. Nuclear endpoint of Wnt signaling: Neoplastic transformation induced by transactivating lymphoid-enhancing factor 1. Proc. Natl. Acad. Sci. USA, 96: 139-144, 1999.
22. Cross, D. A., Alessi, D. R., Cohen, P., Andejelkovich, M., and Hemmings, B. A. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature (Lond.), 378: 785-789, 1995.
23. Cantley, L., Auger, K., Carpenter, C., Duckworth, B., Granziani, A., Kapeller, R., and Soltoff, S. Oncogenes and signal transduction. Cell, 64: 281-302, 1991.
24. Barbier, M., Attoub, S., Calvez, R., Laffargue, M., Jarry, A., Mareel, M., Airuda, F., Gespach, C., Wu, D., Lu, B., Hirsch, E., and Wymann, M. P. Tumor biology. Weakening link to colorectal cancer? Nature (Lond.), 413: 796, 2001.
25. Chung, C. Y., Potikyan, G., and Firtel, R. A. Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa. Mol. Cell, 7: 937-947, 2001.
26. Kawano, Y., Okamoto, I., Murakami, D., Itoh, H., Yoshida, M., Ueda, S., and Saya, H. Ras oncoprotein induces CD44 cleavage through phosphoinositide 3-OH kinase and the rho family of small G proteins. J. Biol. Chem., 275: 29628-29635, 2000.
27. Yeates, L. C., Gallegos, A., Kozikowski, A. P., and Powis, G. Down regulation of the expression of the p110, p85 and p55 subunits of phosphatidylinositol 3-kinase during colon cancer cell anchorage-independent growth. Anticancer Res., 19: 4171-4176, 1999.
28. Datta, S. R., Dudek, H., Tao, X., Masters, S., Fu, H., Gotoh, Y., and Greenberg, M. E. Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell, 91: 231-241, 1997.
29. Cardone, M. H., Roy, N., Stennicke, H. R., Salvesen, G. S., Franke, T. F., Stanbridge, E., Frisch, S., and Reed, J. C. Regulation of cell death protease caspase-9 by phosphorylation. Science (Wash. DC), 282: 1318-1321, 1998.
30. Hart, M. J., de la Santos, R., Albert, I. N., Rubinfeld, B., and Polakis, P. Down-regulation of β-catenin by human axin and its association with the APC tumor suppressor, β-catenin and GSK-3β. Curr. Biol., 8: 573-581, 1993.
31. Sakanaka, C., Weiss. J. B., and Williams, L. T. Binding of βcatenin and glycogen synthase kinase-3β by axin and inhibition of β-catenin-mediated transcription. Proc. Natl. Acad. Sci. U S A. 95: 3020-3023, 1997.
32. Suzuki, A., de la Pompa, J. L., Stambolic. V., Elia, A. J., Sasaki, T., Barco-Barrantes, D. L., Ho, A., Wakeham, A., Itie, A., Khoo, W., Fukumoto, M., and Mak, T. W. High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice. Curr. Biol., 8: 1169-1178, 1998.
33. Stambolic, V., Suzuki, A., de la Pompa, J., Brothers, G. M., Mirtsos, C., Sasaki, T., Ruland, J., Penninger, J. M., Siderovski, D. P., and Mak, T. W. Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell, 95: 29-39, 1998.