Protein engineering of oxygenases for biocatalysis

Current Opinion in Chemical Biology

Volumn 6, Issue 2, 2002, Pages 130-135

  Cirino, Patrick C ^a   Arnold, Frances H ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORIDE PEROXIDASE; CYTOCHROME P450; DIOXYGENASE; ENZYME; OXIDOREDUCTASE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; OXYGENASE;

BIOCATALYST; BIOREMEDIATION; CATALYSIS; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; HUMAN; NONHUMAN; REVIEW; SYNTHESIS; ANIMAL; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; GENETICS; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING;

ANIMALS; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; HUMANS; OXYGENASES; PROTEIN ENGINEERING;

EID: 0036525713     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(02)00305-8     Document Type: Review

References (54)

1. Protein engineering of cytochromes P-450
2. Enzymatic dihydroxylation of aromatics in enantioselective synthesis: Expanding asymmetric methodology
3. Engineering dioxygenases for efficient degradation of environmental pollutants
4. Biochemical and genetic bases of microbial degradation of polychlorinated biphenyls (PCBs)
5. Selective oxidations catalyzed by peroxidases
6. Selective oxygen transfer catalysed by heme peroxidases: Synthetic and mechanistic aspects
7. Coding nucleotide, 5′ regulatory, and deduced amino acid sequences of P-450BM-3, a single peptide cytochrome P-450:NADPH-P-450 reductase from Bacillus megaterium
8. Fatty acid monooxygenation by cytochrome P-450BM-3
9. The highly stereoselective oxidation of polyunsaturated fatty acids by cytochrome P450 BM-3
10. A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A
11. Cost-effective whole-cell assay for laboratory evolution of hydroxylases in Escherichia coli
12. Directed evolution of a cytochrome P450 monooxygenase for alkane oxidation
13. A single mutation in cytochrome P450 BM3 changes substrate orientation in a catalytic intermediate and the regiospecificity of hydroxylation
14. P450 in biotechnology: Zinc driven omega-hydroxylation of p-nitrophenoxydodecanoic acid using P450BM-3 F87A as a catalyst
15. 2 inactivation of cytochrome P450BM-3
16. Directed evolution of the fatty-acid hydyoxylase P450BM-3 into an indole-hydroxylating catalyst
17. A P450BM-3 mutant hydroxylates alkanes, cycloalkanes, arenes and heteroarenes
18. Modification of the fatty acid specificity of cytochrome P450BM-3 from Bacillus megaterium by directed evolution: A validated assay
19. Rational evolution of a medium chain-specific cytochrome P-450 BM-3 variant
20. Engineering cytochrome P450BM-3 for oxidation of polycyclic aromatic hydrocarbons
21. Protein engineering of Bacillus megaterium CYP102 - The oxidation of polycyclic aromatic hydrocarbons
22. Rational re-design of the substrate binding site of flavocytochrome P450BM3
23. Residue size at position 87 of cytochrome P450BM-3 determines its stereoselectivity in propylbenzene and 3-chlorostyrene oxidation
24. Oxidation of polychlorinated benzenes by genetically engineered CYP101 (cytochrome P450(cam))
25. Engineering the haem monooxygenase cytochrome P450(cam) for monoterpene oxidation
26. Expression, purification, and biochemical characterization of a human cytochrome P450CYP2D6-NADPH cytochrome P450 reductase fusion protein
27. Engineering of a functional human NADH-dependent cytochrome P450 system
28. Engineering artificial redox chains by molecular 'Lego'
29. Control of electron transfer in neuronal NO synthase
30. Microsomal cytochrome P450 2C5: Comparison to microbial P450s and unique features
31. Analysis of mammalian cytochrome P450 structure and function by site-directed mutagenesis
32. Practical application of mammalian cytochrome P450
33. High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: Statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2
34. Rate-determining steps in phenacetin oxidations by human cytochrome P450 1A2 and selected mutants
35. Cytochrome P450 (CYP) mutants and substrate-specificity alterations: Segment-directed mutagenesis applied to human CYP1A1
36. Random mutagenesis of human cytochrome P450 2A6 and screening with indole oxidation products
37. Libraries of hybrid proteins from distantly-related sequences
38. Design of a novel P450: A functional bacterial-human cytochrome P450 chimera
39. Alternative splicing determines the function of CYP4F3 by switching substrate specificity
40. Changing the substrate reactivity of 2-hydroxybiphenyl 3-monooxygenase from Pseudomonas azelaica HBP1 by directed evolution
41. Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation
42. An integrated process for the production of toxic catechols from toxic phenols based on a designer biocatalyst
43. Monitoring trichloroethylene mineralization by Pseudomonas cepacia G4 PR1
44. Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase
45. Multiple mutations at the active site of naphthalene dioxygenase affect regioselectivity and enantioselectivity
46. Emergence of multifunctional oxygenase activities by random priming recombination
47. Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase
48. Directed evolution of biphenyl dioxygenase: Emergence of enhanced degradation capacity for benzene, toluene, and alkylbenzenes
49. Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate
50. A versatile high throughput screen for dioxygenase activity using solid-phase digital imaging
51. Altering catalytic properties of 3-chlorocatechol-oxidizing extradiol dioxygenase from Sphingomonas xenophaga BN6 by random mutagenesis
52. Directed evolution of chloroperoxidase for improved epoxidation and chlorination catalysis
53. Engineering chloroperoxidase for activity and stability
54. Expression of the Caldariomyces fumago chloroperoxidase in Aspergillus niger and characterization of the recombinant enzyme