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Volumn 15, Issue 4-6, 2001, Pages 123-133
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Modification of the fatty acid specificity of cytochrome P450 BM-3 from Bacillus megaterium by directed evolution: A validated assay
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Author keywords
Bacillus megaterium; Colorimetric assay system; Cytochrome P450 CYP102; Directed evolution; Fatty acid hydroxylation; High throughput screening
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Indexed keywords
ASSAYS;
CATALYSIS;
ENZYMES;
HYDROXYLATION;
MUTAGENESIS;
SUBSTRATES;
CYTOCHROME;
FATTY ACIDS;
4 NITROPHENOL;
CYTOCHROME P450;
CYTOCHROME P450 102;
DECANOIC ACID;
ENZYME VARIANT;
FATTY ACID;
LAURIC ACID;
OCTANOIC ACID;
OMEGA (4 NITROPHENOXY)DECANOIC ACID;
OMEGA (4 NITROPHENOXY)OCTANOIC ACID;
UNCLASSIFIED DRUG;
ARTICLE;
BACILLUS MEGATERIUM;
CATALYSIS;
COLORIMETRY;
CONTROLLED STUDY;
ENZYME ACTIVITY;
ENZYME ASSAY;
ENZYME ISOLATION;
ENZYME MODIFICATION;
ENZYME PURIFICATION;
ENZYME SPECIFICITY;
FATTY ACID SYNTHESIS;
HYDROXYLATION;
MOLECULAR EVOLUTION;
NONHUMAN;
SPECTROPHOTOMETRY;
STEREOCHEMISTRY;
VALIDATION PROCESS;
BACILLUS MEGATERIUM;
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EID: 0035501591
PISSN: 13811177
EISSN: None
Source Type: Journal
DOI: 10.1016/S1381-1177(01)00015-7 Document Type: Article |
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Times cited : (48)
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References (9)
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