Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: Generic, parallel, and scalable protein purification

Protein Expression and Purification

Volumn 24, Issue 2, 2002, Pages 313-322

  Blank, Kerstin ^a,c   Lindner, Peter ^a   Diefenbach, Beate ^b   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b MORPHOSYS AG   (Germany)

^c Nanotype GmbH   (Germany)

Author keywords

Chitin binding domain; Directed protein immobilization; Fusion protein; Immunoaffinity chromatography; Recombinant antibody fragment; scFv

Indexed keywords

BACTERIA (MICROORGANISMS);

IMMUNOGLOBULIN FRAGMENT; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; GENETICS; IMMUNOADSORBENT; IMMUNOLOGY; INSTRUMENTATION; ISOLATION AND PURIFICATION; METHODOLOGY;

CHROMATOGRAPHY, AFFINITY; IMMUNOGLOBULIN FRAGMENTS; IMMUNOSORBENTS; RECOMBINANT PROTEINS;

EID: 0036517707     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1575     Document Type: Article

References (50)

1. Cutler, P. (1996) Affinity chromatography. Methods Mol. Biol. 59, 157-168.
2. Jack, G. W., and Beer, D. J. (1996) Immunoaffinity chromatography. Methods Mol. Biol. 59, 187-196.
3. Jones, C., Patel, A., Griffin, S., Martin, J., Young, P., O'Donnell, K., Silverman, C., Porter, T., and Chaiken, I. (1995) Current trends in molecular recognition and bioseparation. J. Chromatogr. A 707, 3-22.
4. Sii, D., and Sadana, A. (1991) Bioseparation using affinity techniques. J. Biotechnol. 19, 83-98.
5. Ford, C. F., Suominen, I., and Glatz, C. E. (1991) Fusion tails for the recovery and purification of recombinant proteins. Protein Express. Purif. 2, 95-107.
6. Nilsson, J., Ståhl, S., Lundeberg, J., Uhlén, M., and Nygren, P. A. (1997) Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Express. Purif. 11, 1-16.
7. Smith, G. P., and Scott, J. K. (1993) Libraries of peptides and proteins displayed on filamentous phage. Methods Enzymol. 217, 228-257.
8. Winter, G., Griffiths, A. D., Hawkins, R. E., and Hoogenboom, H. R. (1994) Making antibodies by phage display technology. Annu. Rev. Immunol. 12, 433-455.
9. Hanes, J., Schaffitzel, C., Knappik, A., and Plückthun, A. (2000) Picomolar affinity antibodies from a fully synthetic naïve library selected and evolved by ribosome display. Nature Biotechnol. 18, 1287-1292.
10. Plückthun, A., Schaffitzel, C., Hanes, J., and Jermutus, L. (2000) In vitro selection and evolution of proteins. Adv. Protein Chem. 55, 367-403.
11. Mössner, E., Koch, H., and Plückthun, A. (2001) Fast selection of antibodies without antigen purification: Adaptation of the protein fragment complementation assay to select antigen-antibody pairs. J. Mol. Biol. 308, 115-122.
12. Knappik, A., Ge, L., Honegger, A., Pack, P., Fischer, M., Wellnhofer, G., Hoess, A., Wolle, J., Plückthun, A., and Virnekäs, B. (2000) Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. J. Mol. Biol. 296, 57-86.
13. Vaughan, T. J., Williams, A. J., Pritchard, K., Osbourn, J. K., Pope, A. R., Earnshaw, J. C., McCafferty, J., Hodits, R. A., Wilton, J., and Johnson, K. S. (1996) Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nature Biotechnol. 14, 309-314.
14. Arnold-Schild, D., Kleist, C., Welschof, M., Opelz, G., Rammensee, H. G., Schild, H., and Terness, P. (2000) One-step single-chain Fv recombinant antibody-based purification of gp96 for vaccine development. Cancer Res. 60, 4175-4178.
15. Berry, M. J., Davies, J., Smith, C. G., and Smith, I. (1991) Immobilization of Fv antibody fragments on porous silica and their utility in affinity chromatography. J. Chromatogr. 587, 161-169.
16. Berry, M. J., and Pierce, J. J. (1993) Stability of immunoadsorbents comprising antibody fragments: Comparison of Fv fragments and single-chain Fv fragments. J. Chromatogr. 629, 161-168.
17. Molloy, P., Brydon, L., Porter, A. J., and Harris, W. J. (1995) Separation and concentration of bacteria with immobilized anti-body fragments. J. Appl. Bacteriol. 78, 359-365.
18. Turkova, J. (1999) Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function. J. Chromatogr. B Biomed. Sci. Appl. 722, 11-31.
19. Kleymann, G., Ostermeier, C., Ludwig, B., Skerra, A., and Michel, H. (1995) Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes. Bio/Technology (N. Y.) 13, 155-160.
20. Weiss, E., Chatellier, J., and Orfanoudakis, G. (1994) In vivo biotinylated recombinant antibodies: construction, characterization, and application of a bifunctional Fab-BCCP fusion protein produced in Escherichia coli. Protein Express. Purif. 5, 509-517.
21. Chong, S., Mersha, F. B., Comb, D. G., Scott, M. E., Landry, D., Vence, L. M., Perler, F. B., Benner, J., Kucera, R. B., Hirvonen, C. A., Pelletier, J. J., Paulus, H., and Xu, M. Q. (1997) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192, 271-281.
22. Shpigel, E., Elias, D., Cohen, I. R., and Shoseyov, O. (1998) Production and purification of a recombinant human hsp60 epitope using the cellulose-binding domain in Escherichia coli. Protein Express. Purif. 14, 185-191.
23. Ramirez, C., Fung, J., Miller, R. C., Jr., Antony, R., Warren, J., and Kilburn, D. G. (1993) A bifunctional affinity linker to couple antibodies to cellulose. Bio/Technology (N. Y.) 11, 1570-1573.
24. Shpigel, E., Goldlust, A., Efroni, G., Avraham, A., Eshel, A., Dekel, M., and Shoseyov, O. (1999) Immobilization of recombinant heparinase I fused to cellulose-binding domain. Biotechnol. Bioeng. 65, 17-23.
25. Hochuli, E., Bannwarth, W., Döbeli, H., Gentz, R., and Stüber, D. (1988) Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology (N. Y.) 6, 1321-1325.
26. Lindner, P., Guth, B., Wülfing, C., Krebber, C., Steipe, B., Müller, F., and Plückthun, A. (1992) Purification of native proteins from the cytoplasm and periplasm of Escherichia coli using IMAC and histidine tails: a comparison of proteins and protocols. Methods: Companion Methods Enzymol. 4, 41-56.
27. Krebber, A., Bornhauser, S., Burmester, J., Honegger, A., Willuda, J., Bosshard, H. R., and Plückthun, A. (1997) Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J. Immunol. Methods 201, 35-55.
28. Pack, P., and Plückthun, A. (1992) Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric Fv fragments with high avidity in Escherichia coli. Biochemistry 31, 1579-1584.
29. Bothmann, H., and Plückthun, A. (1998) Selection for a periplasmic factor improving phage display and functional periplasmic expression. Nature Biotechnol. 16, 376-380.
30. Ikegami, T., Okada, T., Hashimoto, M., Seino, S., Watanabe, T., and Shirakawa, M. (2000) Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1. J. Biol. Chem. 275, 13654-13661.
31. Krebs, B., Rauchenberger, R., Reiffert, S., Rothe, C., Tesar, M., Thomassen, E., Cao, M., Dreier, T., Fischer, D., Höß, A., Inge, L., Knappik, A., Marget, M., Pack, P., Meng, X., Schier, R., Söhlemann, P., Winter, J., Wölle, J., and Kretzschmar, T. (2001) High-throughput generation and engineering of recombinant human antibodies. J. Immunol. Methods 254, 67-84.
32. Bass, S., Gu, Q., and Christen, A. (1996) Multicopy suppressors of prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB), DksA, and a truncated R1pA. J. Bacteriol. 178, 1154-1161.
33. Ramm, K., and Plückthun, A. (2000) The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. II. Isomerase-independent chaperone activity in vitro. J. Biol. Chem. 275, 17106-17113.
34. Forrer, P., and Jaussi, R. (1998) High-level expression of soluble heterologous proteins in the cytoplasm of Escherichia coli by fusion to the bacteriophage lambda head protein D. Gene 224, 45-52.
35. Yang, F., Forrer, P., Dauter, Z., Conway, J. F., Cheng, N., Cerritelli, M. E., Steven, A. C., Plückthun, A., and Wlodawer, A. (2000) Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD. Nature Struct. Biol. 7, 230-237.
36. Lindner, P., Bauer, K., Krebber, A., Nieba, L., Kremmer, E., Krebber, C., Honegger, A., Klinger, B., Mocikat, R., and Plückthun, A. (1997) Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques 22, 140-149.
37. Ge, L., Knappik, A., Pack, P., Freund, C., and Plückthun, A. (1995) in "Antibody Engineering" (Borrebaeck, C. A. K., Ed.), 2nd ed, pp. 229-266, Oxford Univ. Press, New York, NY
38. Knappik, A., and Plückthun, A. (1994) An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments. Biotechniques 17, 754-761.
39. Watanabe, T., Ito, Y., Yamada, T., Hashimoto, M., Sekine, S., and Tanaka, H. (1994) The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation. J. Bacteriol. 176, 4465-4472.
40. Linder, M., Salovuori, I., Ruohonen, L., and Teeri, T. T. (1996) Characterization of a double cellulose-binding domain: Synergistic high affinity binding to crystalline cellulose. J. Biol. Chem. 271, 21268-21272.
41. Linder, M., Nevanen, T., Söderholm, L., Bengs, O., and Teeri, T. T. (1998) Improved immobilization of fusion proteins via cellulose-binding domains. Biotechnol. Bioeng. 60, 642-647.
42. Carrard, G., Koivula, A., Söderlund, H., and Beguin, P. (2000) Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose. Proc. Natl. Acad. Sci. USA 97, 10342-10347.
43. Bothmann, H., and Plückthun, A. (2000) The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines. J. Biol. Chem. 275, 17100-17105.
44. Crameri, A., Whitehorn, E. A., Tate, E., and Stemmer, W. P. (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechnol. 14, 315-319.
45. Nieba, L., Nieba-Axmann, S. E., Persson, A., Hamalainen, M., Edebratt, F., Hansson, A., Lidholm, J., Magnusson, K., Karlsson, A. F., and Plückthun, A. (1997) BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip. Anal. Biochem. 252, 217-228.
46. Carter, P., Kelley, R. F., Rodrigues, M. L., Snedecor, B., Covarrubias, M., Velligan, M. D., Wong, W. L. T., Rowland, A. M., Kotts, C. E., Carver, M. E., Yang, M., Bourell, J. H., Shepard, H. M., and Henner, D. (1992) High level Escherichia coli expression and production of a bivalent humanized antibody fragment. Bio/Technology (N. Y.) 10, 163-167.
47. Inouye, H., Michaelis, S., Wright, A., and Beckwith, J. (1981) Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro. J. Bacteriol. 146, 668-675.
48. M and Taq DNA polymerase. J. Biotechnol. 80, 45-54.
49. Sproule, K., Morrill, P., Pearson, J. C., Burton, S. J., Hejnæs, K. R., Valore, H., Ludvigsen, S., and Lowe, C. R. (2000) New strategy for the design of ligands for the purification of pharmaceutical proteins by affinity chromatography. J. Chromatogr. B Biomed. Sci. Appl. 740, 17-33.
50. Lanio, T., Jeltsch, A., and Pingoud, A. (2000) Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis. Biotechniques 29, 338-342.