-
1
-
-
0026071781
-
Metal-affinity separations: A new dimension in protein processing
-
Arnold, F.H. 1991. Metal-affinity separations: a new dimension in protein processing. Bio/Technology 9:151-156.
-
(1991)
Bio/Technology
, vol.9
, pp. 151-156
-
-
Arnold, F.H.1
-
2
-
-
0021321955
-
A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots
-
Blake, M.S., K.H. Johnston, G.J. Russel-Jones and E.C. Gotschlich. 1984. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal. Biochem. 136:175-179.
-
(1984)
Anal. Biochem.
, vol.136
, pp. 175-179
-
-
Blake, M.S.1
Johnston, K.H.2
Russel-Jones, G.J.3
Gotschlich, E.C.4
-
3
-
-
0028982533
-
Reporter enzyme-nitrilo-triacetic acid-nickel conjugates: Reagents for detecting histidine-tagged proteins
-
Botting, C.H. and R.E. Randall. 1995. Reporter enzyme-nitrilo-triacetic acid-nickel conjugates: reagents for detecting histidine-tagged proteins. BioTechniques 19:362-363.
-
(1995)
BioTechniques
, vol.19
, pp. 362-363
-
-
Botting, C.H.1
Randall, R.E.2
-
4
-
-
0028941913
-
Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: Application to anti-HBsAg detection
-
Carrier, A., F. Ducancel, N.B. Settiawan, L. Cattolico, B. Maillère, M. Léonetti, P. Drevet, A. Ménez and J.-C. Boulain. 1995. Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection. J. Immunol. Methods 181:177-186.
-
(1995)
J. Immunol. Methods
, vol.181
, pp. 177-186
-
-
Carrier, A.1
Ducancel, F.2
Settiawan, N.B.3
Cattolico, L.4
Maillère, B.5
Léonetti, M.6
Drevet, P.7
Ménez, A.8
Boulain, J.-C.9
-
5
-
-
0028836167
-
Purification of bacterially expressed single chain Fv antibodies for clinical applications using metal chelate chromatography
-
Casey, J.L., P.A. Keep, K.A. Chester, L. Robson, R.E. Hawkins and R.H.J. Begent. 1995. Purification of bacterially expressed single chain Fv antibodies for clinical applications using metal chelate chromatography. J. Immunol. Methods 179:105-116.
-
(1995)
J. Immunol. Methods
, vol.179
, pp. 105-116
-
-
Casey, J.L.1
Keep, P.A.2
Chester, K.A.3
Robson, L.4
Hawkins, R.E.5
Begent, R.H.J.6
-
6
-
-
0029145047
-
The DNA-binding domain of HIV-1 integrase has an SH3-like fold
-
Eijkelenboom, A.P.A.M., R.A.P. Lutzke, R. Boelens, R.H.A. Plasterk, R. Kaptein and K. Hård. 1995. The DNA-binding domain of HIV-1 integrase has an SH3-like fold. Nature Struct. Biol. 2:807-810.
-
(1995)
Nature Struct. Biol.
, vol.2
, pp. 807-810
-
-
Eijkelenboom, A.P.A.M.1
Lutzke, R.A.P.2
Boelens, R.3
Plasterk, R.H.A.4
Kaptein, R.5
Hård, K.6
-
7
-
-
0001587390
-
Expressing antibodies in Escherichia coli
-
C.A.K. Borrebaeck (Ed.), Oxford University Press, New York
-
Ge, L., A. Knappik, P. Pack, C. Freund and A. Plückthun. 1995. Expressing antibodies in Escherichia coli, p. 229-266. In C.A.K. Borrebaeck (Ed.), Antibody Engineering: Breakthroughs in Molecular Biology. Oxford University Press, New York.
-
(1995)
Antibody Engineering: Breakthroughs in Molecular Biology
, pp. 229-266
-
-
Ge, L.1
Knappik, A.2
Pack, P.3
Freund, C.4
Plückthun, A.5
-
8
-
-
0023781763
-
Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent
-
Hochuli, E., W. Bannwarth, H. Döbeli, R. Gentz and D. Stüber. 1988. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology 6:1321-1325.
-
(1988)
Bio/Technology
, vol.6
, pp. 1321-1325
-
-
Hochuli, E.1
Bannwarth, W.2
Döbeli, H.3
Gentz, R.4
Stüber, D.5
-
9
-
-
0027374551
-
26-10 Fah-digoxin complex: Affinity and specificity due to surface complementarity
-
Jeffrey, P.D., R.K. Strong, L.C. Sieker, C.Y.Y. Chang, R.L. Campbell, G.A. Petsko, E. Haber, M.N. Margolies and S. Sheriff. 1993. 26-10 Fah-digoxin complex: affinity and specificity due to surface complementarity. Proc. Natl. Acad. Sci. USA 90:10310-10314.
-
(1993)
Proc. Natl. Acad. Sci. USA
, vol.90
, pp. 10310-10314
-
-
Jeffrey, P.D.1
Strong, R.K.2
Sieker, L.C.3
Chang, C.Y.Y.4
Campbell, R.L.5
Petsko, G.A.6
Haber, E.7
Margolies, M.N.8
Sheriff, S.9
-
10
-
-
0027978869
-
Real-time competitive kinetic analysis of interactions between low-molecular-weight ligands in solution and surface-immobilized receptors
-
Karlsson, R. 1994. Real-time competitive kinetic analysis of interactions between low-molecular-weight ligands in solution and surface-immobilized receptors. Anal. Biochem. 221:142-151.
-
(1994)
Anal. Biochem.
, vol.221
, pp. 142-151
-
-
Karlsson, R.1
-
11
-
-
0028353230
-
Production, purification and immunogenicity of a malaria transmission-blocking vaccine candidate: TBV25H expressed in yeast and purified using nickel-NTA agarose
-
Kaslow, D.C. and J. Shiloach. 1994. Production, purification and immunogenicity of a malaria transmission-blocking vaccine candidate: TBV25H expressed in yeast and purified using nickel-NTA agarose. Bio/Technology 12:494-499.
-
(1994)
Bio/Technology
, vol.12
, pp. 494-499
-
-
Kaslow, D.C.1
Shiloach, J.2
-
12
-
-
0018726056
-
A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines
-
Kearney, J.F., A. Radbruch, B. Liesegang and K. Rajewski. 1979. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 121:1548-1550.
-
(1979)
J. Immunol.
, vol.121
, pp. 1548-1550
-
-
Kearney, J.F.1
Radbruch, A.2
Liesegang, B.3
Rajewski, K.4
-
13
-
-
0028073310
-
An improved affinity tag based on the FLAG® peptide for the detection and purification of recombinant antibody fragments
-
Knappik, A. and A. Plückthun. 1994. An improved affinity tag based on the FLAG® peptide for the detection and purification of recombinant antibody fragments. BioTechniques 17:754-761.
-
(1994)
BioTechniques
, vol.17
, pp. 754-761
-
-
Knappik, A.1
Plückthun, A.2
-
14
-
-
0016756272
-
Continuous cultures of fused cells secreting antibody of predefined specificity
-
Köhler, G. and C. Milstein. 1975. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature (London) 256:495-497.
-
(1975)
Nature (London)
, vol.256
, pp. 495-497
-
-
Köhler, G.1
Milstein, C.2
-
15
-
-
0029585566
-
Co-selection of cognate antibody-antigen pairs by selectively-infective phages
-
Krebber, C., S. Spada, D. Desplancq and A. Plückthun. 1995. Co-selection of cognate antibody-antigen pairs by selectively-infective phages. FEBS Lett, 377:227-231.
-
(1995)
FEBS Lett
, vol.377
, pp. 227-231
-
-
Krebber, C.1
Spada, S.2
Desplancq, D.3
Plückthun, A.4
-
16
-
-
0024515966
-
Neutralization of immunosuppression by antibodies against variable as well as constant regions of monoclonal anti-Thy-1 antibodies and their ability to be suppressed by initial T cell depletion
-
Kremmer, E., S. Thierfelder, U. Kummer, R. Lederer and J. Mysliwietz. 1989. Neutralization of immunosuppression by antibodies against variable as well as constant regions of monoclonal anti-Thy-1 antibodies and their ability to be suppressed by initial T cell depletion. Transplantation 47:641-646.
-
(1989)
Transplantation
, vol.47
, pp. 641-646
-
-
Kremmer, E.1
Thierfelder, S.2
Kummer, U.3
Lederer, R.4
Mysliwietz, J.5
-
17
-
-
0003044623
-
Purification of native proteins from the cytoplasm and periplasm of Escherichia coli using IMAC and histidine tails: A comparison of proteins and protocols
-
Lindner, P., B. Guth, C. Wülfing, C. Krebber, B. Steipe, F. Müller and A. Plückthun. 1992. Purification of native proteins from the cytoplasm and periplasm of Escherichia coli using IMAC and histidine tails: a comparison of proteins and protocols. Methods: A Companion to Methods in Enzymology 4-41-56.
-
(1992)
Methods: A Companion to Methods in Enzymology
, vol.4
, pp. 41-56
-
-
Lindner, P.1
Guth, B.2
Wülfing, C.3
Krebber, C.4
Steipe, B.5
Müller, F.6
Plückthun, A.7
-
18
-
-
0028204414
-
A mouse model for the preclinical evaluation of immunosuppressive effector functions of human isotypes. The human IgG1 isotype is superior to IgG3
-
Mocikat, R., G. Kütemeier, G. Hoffmann-Fezer and S. Thierfelder. 1994. A mouse model for the preclinical evaluation of immunosuppressive effector functions of human isotypes. The human IgG1 isotype is superior to IgG3. Transplantation 57:405-411.
-
(1994)
Transplantation
, vol.57
, pp. 405-411
-
-
Mocikat, R.1
Kütemeier, G.2
Hoffmann-Fezer, G.3
Thierfelder, S.4
-
19
-
-
0028809418
-
High-level production and one-step purification of biologically active human growth hormone in Escherichia coli
-
Mukhija, R., P. Rupa, D. Pillai and L.C. Garg. 1995. High-level production and one-step purification of biologically active human growth hormone in Escherichia coli. Gene 165:303-306.
-
(1995)
Gene
, vol.165
, pp. 303-306
-
-
Mukhija, R.1
Rupa, P.2
Pillai, D.3
Garg, L.C.4
-
20
-
-
0030039913
-
Competition BIAcore for measuring true affinities: Large differences to values determined from binding kinetics
-
Nieba, L., A. Krebber and A. Plückthun. 1996. Competition BIAcore for measuring true affinities: large differences to values determined from binding kinetics. Anal. Biochem. 234:155-165.
-
(1996)
Anal. Biochem.
, vol.234
, pp. 155-165
-
-
Nieba, L.1
Krebber, A.2
Plückthun, A.3
-
21
-
-
0028006072
-
Antibody fragments from a "single pot" phage display library as immunochemical reagents
-
Nissim, A., H.R. Hoogenboom, I.M. Tomlinson, G. Flynn, C. Midgley, D. Lane and G. Winter. 1994. Antibody fragments from a "single pot" phage display library as immunochemical reagents. EMBO J. 13:692-698.
-
(1994)
EMBO J.
, vol.13
, pp. 692-698
-
-
Nissim, A.1
Hoogenboom, H.R.2
Tomlinson, I.M.3
Flynn, G.4
Midgley, C.5
Lane, D.6
Winter, G.7
-
22
-
-
0028287188
-
Engineering proteins to facilitate bioprocessing
-
Nygren, P.-Å., S. Ståhl and M. Uhlén. 1994. Engineering proteins to facilitate bioprocessing. Trends Biotechnol. 12:184-188.
-
(1994)
Trends Biotechnol.
, vol.12
, pp. 184-188
-
-
Nygren, P.-Å.1
Ståhl, S.2
Uhlén, M.3
-
23
-
-
0003046374
-
Producing antibodies in Escherichia coli: From PCR to fermentation
-
J. McCafferty and H.R. Hoogenboom (Eds.), IRL Press, Oxford
-
Plückthun, A., A. Krebber, C. Krebber, U. Horn, U. Knüpfer, R. Wenderoth, L. Nieba, K. Proba and D. Riesenberg. 1996. Producing antibodies in Escherichia coli: from PCR to fermentation, p. 203-252. In J. McCafferty and H.R. Hoogenboom (Eds.), Antibody Engineering: A Practical Approach. IRL Press, Oxford.
-
(1996)
Antibody Engineering: A Practical Approach
, pp. 203-252
-
-
Plückthun, A.1
Krebber, A.2
Krebber, C.3
Horn, U.4
Knüpfer, U.5
Wenderoth, R.6
Nieba, L.7
Proba, K.8
Riesenberg, D.9
-
24
-
-
0028789440
-
A highly specific and sensitive monoclonal antibody detecting histidine-tagged recombinant proteins
-
Pogge v. Strandmann, E.P., C. Zoidl, H. Nakhei, B. Holewa, R. Pogge v. Strandmann. P. Lorenz, L. Klein-Hitpaß and G.U. Ryffel. 1995. A highly specific and sensitive monoclonal antibody detecting histidine-tagged recombinant proteins. Protein Eng. 8:733-735.
-
(1995)
Protein Eng.
, vol.8
, pp. 733-735
-
-
Pogge V Strandmann, E.P.1
Zoidl, C.2
Nakhei, H.3
Holewa, B.4
Pogge V Strandmann, R.5
Lorenz, P.6
Klein-Hitpaß, L.7
Ryffel, G.U.8
-
26
-
-
0027325038
-
Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen
-
Rini, J.M., R.L. Stanfield, E.A. Stura, P.A. Salinas, A.T. Profy and I.A. Wilson. 1993. Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen. Proc. Natl. Acad. Sci. USA 90:6325-6329.
-
(1993)
Proc. Natl. Acad. Sci. USA
, vol.90
, pp. 6325-6329
-
-
Rini, J.M.1
Stanfield, R.L.2
Stura, E.A.3
Salinas, P.A.4
Profy, A.T.5
Wilson, I.A.6
-
27
-
-
0004136246
-
-
Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
-
Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
-
(1989)
Molecular Cloning: A Laboratory Manual, 2nd Ed.
-
-
Sambrook, J.1
Fritsch, E.F.2
Maniatis, T.3
-
28
-
-
0026644604
-
Immuno-PCR: Very sensitive antigen detection by means of specific antibody-DNA conjugates
-
Sano, T., C.L. Smith and C.R. Cantor. 1992. Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. Science 258:120-122.
-
(1992)
Science
, vol.258
, pp. 120-122
-
-
Sano, T.1
Smith, C.L.2
Cantor, C.R.3
-
29
-
-
0015335214
-
Immunoglobulin and θ-bearing murine leukemias and lymphomas
-
Shevach, E.M., J.D. Stobo and I. Green. 1972. Immunoglobulin and θ-bearing murine leukemias and lymphomas. J. Immunol. 108:1146-1151.
-
(1972)
J. Immunol.
, vol.108
, pp. 1146-1151
-
-
Shevach, E.M.1
Stobo, J.D.2
Green, I.3
-
30
-
-
0027301875
-
Crystal structure of an anticholera toxin peptide complex at 2.3 angstroms
-
Shoham, M. 1993. Crystal structure of an anticholera toxin peptide complex at 2.3 angstroms. J. Mol. Biol. 232:1169-1175.
-
(1993)
J. Mol. Biol.
, vol.232
, pp. 1169-1175
-
-
Shoham, M.1
-
31
-
-
0025951267
-
Preparation of clinical grade proteins produced by recombinant DNA technologies
-
Takacs, B.J. and M.-F. Girard. 1991. Preparation of clinical grade proteins produced by recombinant DNA technologies. J. Immunol. Methods 143:231-240.
-
(1991)
J. Immunol. Methods
, vol.143
, pp. 231-240
-
-
Takacs, B.J.1
Girard, M.-F.2
-
32
-
-
84988119922
-
Comparison of semi-dry and conventional tank-buffer electrotransfer of proteins from polyacrylamide gels to nitrocellulose membranes
-
Tovey, E.R. and B.A. Baldo. 1987. Comparison of semi-dry and conventional tank-buffer electrotransfer of proteins from polyacrylamide gels to nitrocellulose membranes. Electrophoresis 8:384-387.
-
(1987)
Electrophoresis
, vol.8
, pp. 384-387
-
-
Tovey, E.R.1
Baldo, B.A.2
-
33
-
-
14744298086
-
Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor
-
Weis, W., I.-M. Harwerth, M. Zwickl, N. Hardman, B. Groner and N.E. Hynes. 1992. Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor. Bio/Technology 10:1128-1132.
-
(1992)
Bio/Technology
, vol.10
, pp. 1128-1132
-
-
Weis, W.1
Harwerth, I.-M.2
Zwickl, M.3
Hardman, N.4
Groner, B.5
Hynes, N.E.6
-
34
-
-
0029122824
-
Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody
-
Zentgraf, H., M. Frey, S. Schwinn, C. Tessmer, B. Willemann, Y. Samstag and I. Velhagen. 1995. Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody. Nucleic Acids Res. 23:3347-3348.
-
(1995)
Nucleic Acids Res.
, vol.23
, pp. 3347-3348
-
-
Zentgraf, H.1
Frey, M.2
Schwinn, S.3
Tessmer, C.4
Willemann, B.5
Samstag, Y.6
Velhagen, I.7
-
35
-
-
0027495263
-
Crystallization and preliminary X-ray studies of extracellular signal-regulated kinase-2/MAP kinase with an incorporated His-tag
-
Zhang, F., D.J. Robbins, M.H. Cobb and E.J. Goldsmith. 1993. Crystallization and preliminary X-ray studies of extracellular signal-regulated kinase-2/MAP kinase with an incorporated His-tag. J. Mol. Biol. 233:550-552.
-
(1993)
J. Mol. Biol.
, vol.233
, pp. 550-552
-
-
Zhang, F.1
Robbins, D.J.2
Cobb, M.H.3
Goldsmith, E.J.4
|