One-step single-chain Fv recombinant antibody-based purification of gp96 for vaccine development

Cancer Research

Volumn 60, Issue 15, 2000, Pages 4175-4178

  Arnold Schild, D ^a   Kleist, C ^a   Welschof, M ^a   Opelz, G ^a   Rammensee, H G ^a   Schild, H ^a   Terness, P ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CANCER VACCINE; HEAT SHOCK PROTEIN; IMMUNOGLOBULIN FRAGMENT; RECOMBINANT ANTIBODY;

ADOLESCENT; AMINO ACID SEQUENCE; ANIMAL CELL; ANTIGEN PURIFICATION; ARTICLE; CELLULAR IMMUNITY; CHROMATOGRAPHY; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNIZATION; IMMUNOPRECIPITATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; VACCINE PRODUCTION;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODY SPECIFICITY; ANTIGENS, NEOPLASM; ANTIGENS, SURFACE; CANCER VACCINES; CHROMATOGRAPHY, AGAROSE; HEAT-SHOCK PROTEINS; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED C57BL; MICE, INBRED STRAINS; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PRECIPITIN TESTS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; T-LYMPHOCYTES, CYTOTOXIC; TUMOR CELLS, CULTURED;

EID: 0033869693     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Heat shock proteins come of age: Primitive functions acquire new roles in an adaptive world
2. Stress proteins and immunity mediated by cytotoxic T lymphocytes
3. Comparison of tumor-specific immunogenicities of stress-induced proteins gp96, hsp90, and hsp70
4. Cutting edge: Receptor-mediated endocytosis of heat shock proteins by professional antigen-presenting cells
5. Receptor mediated and fluid phase pathways for internalization of the ER Hsp90 chaperone GRP94 in murine macrophages
6. Heat shock proteins transfer peptides during antigen processing and CTL priming
7. Immunotherapy of tumors with autologous tumor-derived heat shock protein preparations
8. Antibody fragments from a 'single pot' phage display library as immunochemical reagents
9. Cross-priming of minor histocompatibility antigen-specific cytotoxic T cells upon immunization with the heat shock protein gp96
10. The repertoire of human germline VH sequences reveals about fifty groups of VH segments with different hypervariable loops
11. Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains
12. First genomic sequence of a human Ig variable λ gene belonging to subgroup III
13. Cloning and sequencing of human immunoglobulin V λ gene segments
14. Affinity purification of tagged recombinant proteins using immobilized single chain Fv fragments
15. Influences of transporter associated with antigen processing (TAP) on the repertoire of peptides associated with the endoplasmic reticulum-resident stress protein gp96
16. Substrate specificity of cathepsins D and E determined by N-terminal and C-terminal sequencing of peptide pools