Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2

Journal of Cell Biology

Volumn 154, Issue 5, 2001, Pages 925-936

  Sudakin, Valery ^a   Chan, Gordon K T ^a   Yen, Tim J ^a  

^a FOX CHASE CANCER CENTER   (United States)

Author keywords

Anaphase promoting complex; hBUBR1; Kinetochore; MAD2; Mitotic checkpoint

Indexed keywords

ANAPHASE PROMOTING COMPLEX; BUB1 RELATED PROTEIN; BUB3 PROTEIN; CELL CYCLE PROTEIN 20; LIGASE; PROTEIN MAD2; UNCLASSIFIED DRUG;

ARTICLE; CELL LYSATE; CENTROMERE; CONTROLLED STUDY; ENZYME ACTIVITY; HELA CELL; HUMAN; HUMAN CELL; INTERPHASE; MITOSIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PURIFICATION; STOICHIOMETRY;

CALCIUM-BINDING PROTEINS; CARRIER PROTEINS; CELL CYCLE PROTEINS; CELL FRACTIONATION; FUNGAL PROTEINS; GENES, CDC; GENES, REPORTER; HELA CELLS; HUMANS; IMMUNOBLOTTING; KINETOCHORES; LIGASES; MACROMOLECULAR SUBSTANCES; MITOSIS; NUCLEAR PROTEINS; PROTEIN KINASES; PROTEINS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; UBIQUITIN-PROTEIN LIGASE COMPLEXES; UBIQUITIN-PROTEIN LIGASES;

EID: 0035802122     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200102093     Document Type: Article

References (48)

1. CENP-E as an essential component of the mitotic checkpoint in vitro
2. Mitotic checkpoint proteins HsMADI and HsMAD2 are associated with nuclear pore complexes in interphase
3. Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1
4. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC
5. The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae
6. Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores
7. Spindle checkpoint protein Xmadl recruits Xmad2 to unattached kinetochores
8. The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins
9. The Drosophilla cell cycle gene fizzy is required for normal degradation of cyclins A and B during mitosis and has homology to the CDC20 gene of Saccharomyces cerevisiae
10. The polo-like kinase Plx1 is required for M phase exit and destruction of mitotic regulators in Xenopus egg extracts
11. The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation
12. Cyclin is degraded by the ubiquitin pathway
13. Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase
14. Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast
15. MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p
16. The Saccharomyces pombe spindle checkpoint protein Mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex
17. The ubiquitin system
18. Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cells
19. S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function
20. Budding yeast Cdc20: A target of the spindle checkpoint
21. Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1
22. Many malian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events
23. Fission yeast Slp1: An effector of the Mad2-dependent spindle checkpoint
24. A 20S complex containing CDC27 and CDC16 catalyses the mitosis-specific conjugation of ubiquitin to cyclin B
25. How proteolysis drives the cell cycle
26. Regulation of APC activity by phosphorylation and regulatory factors
27. Reversible phosphorylation controls the activity of cyclosome-associated cyclin-ubiquitin ligase
28. Feedback control of mitosis in budding yeast
29. Identification of a human mitotic checkpoint gene: hsMAD2
30. MAD2 associates with the cyclosome/anaphase-promoting complex and inhibits its activity
31. The vertebrate cell kinetochore and its roles during mitosis
32. The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores
33. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase
34. Dominant alleles of Saccharomyces cerevisiae CDC20 reveal its role in promoting anaphase
35. Waiting for anaphase: Mad2 and the spindle assembly checkpoint
36. The polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae
37. Phosphorylation of the cyclosome is required for its stimulation by fizzy/cdc20 Biochem
38. Kinetochores and the checkpoint mechanism that monitors for defects in the chromosome segregation machinery
39. The cyclosome, a large complex containing cyclin-selective ubiquitin lygase activity, targets cyclins for destruction at the end of mitosis
40. Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to spindle damage
41. The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase
42. CDC20 and CDH1: A family of substrate-specific activators of APC-dependent proteolysis
43. Mad2 transiently associates with an APC/p55Cdc complex during mitosis
44. Localization of Mad2 to kinatochores depends on microtubule attachment, not tension
45. p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase
46. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase
47. CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint
48. Whose end is destruction: Cell division and the anaphase-promoting complex