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Volumn 278, Issue 5337, 1997, Pages 460-463

CDC20 and CDH1: A family of substrate-specific activators of APC- dependent proteolysis

Author keywords

[No Author keywords available]

Indexed keywords

REGULATOR PROTEIN;

EID: 0030693087     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5337.460     Document Type: Article
Times cited : (737)

References (30)
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    • 0031055137 scopus 로고    scopus 로고
    • Y.-L. Juang et al., ibid. 275, 1311 (1997).
    • (1997) Science , vol.275 , pp. 1311
    • Juang, Y.-L.1
  • 4
    • 0029004815 scopus 로고
    • R. W. King et al., Cell 81, 279 (1995).
    • (1995) Cell , vol.81 , pp. 279
    • King, R.W.1
  • 14
    • 1842275005 scopus 로고
    • _, ibid. 129, 725 (1995).
    • (1995) Development , vol.129 , pp. 725
  • 16
    • 1842270063 scopus 로고    scopus 로고
    • note
    • 35S-labeled Clb2 protein and DNA content analysis were performed as described (7). Protein immunoblot analysis of the total amount of Pds1, Ase1, and Cdc28 was done as described (1, 7).
  • 17
    • 1842379834 scopus 로고    scopus 로고
    • note
    • To generate a GAL-CDC20 fusion, we engineered a Bgl II site at the AUG of CDC20 and ligated it to an Eco RI to Bam HI fragment carrying the GAL1-10 promoter. The fusion was integrated at the URA3 locus.
  • 20
    • 1842305762 scopus 로고    scopus 로고
    • note
    • -6 for the ORF YRC72C, which also encodes a putative WD40-repeat protein.
  • 22
    • 1842388583 scopus 로고    scopus 로고
    • note
    • A 1.8-kb fragment containing the CDH1 gene was isolated from total genomic DNA by polymerase chain reaction. The HIS3 gene was used to replace the CDH1 ORF between the Nhe I and Blp I sites. The 1.8-kb fragment containing the CDH1 gene was also used to generate a GAL-CDH1 fusion that was integrated at the URA3 locus.
  • 23
    • 1842400307 scopus 로고    scopus 로고
    • note
    • 1 phase daughter cells were elutriated as described (28). Nocodazole and hydroxyurea were added at concentrations of 15 μg/ml and 10 mg/ml, respectively.
  • 30
    • 1842392486 scopus 로고    scopus 로고
    • note
    • We thank O. Cohen-Fix, D. Koshland, and D. Pellman for antibodies and plasmids and V. Siegel, O. Cohen-Fix, D. Koshland, D. Pellman, S. Bell, D. Pellman, H. Madhani, T. Orr-Weaver, V. Mayer, A. Page, and M. Lane for helpful discussions and critical reading of the manuscript.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.