-
1
-
-
0030448251
-
-
O. Cohen-Fix, J.-M. Peters, M. W. Kirschner, D. Koshland, Genes Dev. 10, 3081 (1996).
-
(1996)
Genes Dev.
, vol.10
, pp. 3081
-
-
Cohen-Fix, O.1
Peters, J.-M.2
Kirschner, M.W.3
Koshland, D.4
-
2
-
-
0029807944
-
-
R. W. King, R. J. Deshaies, J.-M. Peters, M. W. Kirschner, Science 274, 1652 (1996).
-
(1996)
Science
, vol.274
, pp. 1652
-
-
King, R.W.1
Deshaies, R.J.2
Peters, J.-M.3
Kirschner, M.W.4
-
3
-
-
0031055137
-
-
Y.-L. Juang et al., ibid. 275, 1311 (1997).
-
(1997)
Science
, vol.275
, pp. 1311
-
-
Juang, Y.-L.1
-
4
-
-
0029004815
-
-
R. W. King et al., Cell 81, 279 (1995).
-
(1995)
Cell
, vol.81
, pp. 279
-
-
King, R.W.1
-
6
-
-
0020961333
-
-
T. Evans, E. T. Rosenthal, J. Youngblom, D. Distel, T. Hunt, Cell 33, 389 (1983).
-
(1983)
Cell
, vol.33
, pp. 389
-
-
Evans, T.1
Rosenthal, E.T.2
Youngblom, J.3
Distel, D.4
Hunt, T.5
-
9
-
-
0025325445
-
-
W. G. Whitfield, C. Gonzalez, G. Maldonado-Codina, D. M. Glover, ibid. 9, 2563 (1990).
-
(1990)
EMBO J.
, vol.9
, pp. 2563
-
-
Whitfield, W.G.1
Gonzalez, C.2
Maldonado-Codina, G.3
Glover, D.M.4
-
11
-
-
0029165112
-
-
S. Sigrist, H. Jakobs, R. Stratmann, C. F. Lehner, EMBO J. 14, 4827 (1995).
-
(1995)
EMBO J.
, vol.14
, pp. 4827
-
-
Sigrist, S.1
Jakobs, H.2
Stratmann, R.3
Lehner, C.F.4
-
12
-
-
0025993769
-
-
N. Sethi, M. C. Monteagudo, D. Koshland, E. Hogan, D. J. Burke, Mol. Cell. Biol. 11, 5592 (1991).
-
(1991)
Mol. Cell. Biol.
, vol.11
, pp. 5592
-
-
Sethi, N.1
Monteagudo, M.C.2
Koshland, D.3
Hogan, E.4
Burke, D.J.5
-
14
-
-
1842275005
-
-
_, ibid. 129, 725 (1995).
-
(1995)
Development
, vol.129
, pp. 725
-
-
-
16
-
-
1842270063
-
-
note
-
35S-labeled Clb2 protein and DNA content analysis were performed as described (7). Protein immunoblot analysis of the total amount of Pds1, Ase1, and Cdc28 was done as described (1, 7).
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17
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1842379834
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note
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To generate a GAL-CDC20 fusion, we engineered a Bgl II site at the AUG of CDC20 and ligated it to an Eco RI to Bam HI fragment carrying the GAL1-10 promoter. The fusion was integrated at the URA3 locus.
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20
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1842305762
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note
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-6 for the ORF YRC72C, which also encodes a putative WD40-repeat protein.
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-
-
-
21
-
-
0025183708
-
-
S. F. Altschul, W. Gish, W. Miller, E. W. Myers, D. J. Lipman, J. Mol. Biol. 251, 403 (1990).
-
(1990)
J. Mol. Biol.
, vol.251
, pp. 403
-
-
Altschul, S.F.1
Gish, W.2
Miller, W.3
Myers, E.W.4
Lipman, D.J.5
-
22
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1842388583
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note
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A 1.8-kb fragment containing the CDH1 gene was isolated from total genomic DNA by polymerase chain reaction. The HIS3 gene was used to replace the CDH1 ORF between the Nhe I and Blp I sites. The 1.8-kb fragment containing the CDH1 gene was also used to generate a GAL-CDH1 fusion that was integrated at the URA3 locus.
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23
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1842400307
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note
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1 phase daughter cells were elutriated as described (28). Nocodazole and hydroxyurea were added at concentrations of 15 μg/ml and 10 mg/ml, respectively.
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-
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-
25
-
-
0027527033
-
-
A. Amon, M. Tyers, B. Futcher, K. Nasmyth, Cell 74, 993 (1993).
-
(1993)
Cell
, vol.74
, pp. 993
-
-
Amon, A.1
Tyers, M.2
Futcher, B.3
Nasmyth, K.4
-
29
-
-
0029033504
-
-
S. Irniger, S. Piatti, M. Michaelis, K. Nasmyth, Cell 87, 269 (1995).
-
(1995)
Cell
, vol.87
, pp. 269
-
-
Irniger, S.1
Piatti, S.2
Michaelis, M.3
Nasmyth, K.4
-
30
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1842392486
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note
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We thank O. Cohen-Fix, D. Koshland, and D. Pellman for antibodies and plasmids and V. Siegel, O. Cohen-Fix, D. Koshland, D. Pellman, S. Bell, D. Pellman, H. Madhani, T. Orr-Weaver, V. Mayer, A. Page, and M. Lane for helpful discussions and critical reading of the manuscript.
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