Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase

Journal of Cell Biology

Volumn 141, Issue 5, 1998, Pages 1193-1205

  Gorbsky, Gary J ^a,d   Chen, Rey Huei ^b   Murray, Andrew W ^c  

^a UNIVERSITY OF VIRGINIA   (United States)

^b Cornell University ^*  (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CELL CYCLE PROTEIN; EPITOPE; INHIBITOR PROTEIN;

ANAPHASE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; KERATINOCYTE; KIDNEY CELL; MAMMAL CELL; METAPHASE; MICROINJECTION; MITOSIS; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; PROPHASE; RAT;

ANAPHASE; ANIMALS; ANTIBODIES; ANTIBODIES, MONOCLONAL; CALCIUM-BINDING PROTEINS; CARRIER PROTEINS; CELL CYCLE PROTEINS; CELL LINE; CELLS, CULTURED; CYTOPLASM; EPITOPES; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FUNGAL PROTEINS; HUMANS; MACROPODIDAE; MICROINJECTIONS; MITOSIS; NUCLEAR PROTEINS; PHOSPHOPROTEINS; SWINE;

ANIMALIA; MAMMALIA;

EID: 0032101607     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.141.5.1193     Document Type: Article

References (40)

1. Bajer, A., and J. Mole-Bajer. 1956. Cine-micrographic studies on mitosis in endosperm II. Chromosoma (Berl.). 7:558-607.
2. Campbell, M.S., and G.J. Gorbsky. 1995. Microinjection of mitotic cells with the 3F3/2 antiphosphoepitope antibody delays the onset of anaphase. J. Cell Biol. 129:1195-1204.
3. Chen, R.H., J.C. Waters, E.D. Salmon, and A.W. Murray. 1996. Association of spindle assembly checkpoint component xMAD2 with unattached kinetochores. Science. 274:242-246.
4. Clute, P., and Y. Masui. 1995. Regulation of the appearance of division asynchrony and microtubule-dependent chromosome cycles in Xenopus laevis embryos. Dev. Biol. 171:273-285.
5. Cohen-Fix, O., and D. Koshland. 1997. The metaphase to anaphase transition: avoiding a mid-life crisis. Curr. Opin. Cell Biol. 9:800-806.
6. Cohen-Fix, O., J.M. Peters, M.W. Kirschner, and D. Koshland. 1996. Anaphase initiation in Saccharomyces cerevisiae is controlled by the apc-dependent degradation of the anaphase inhibitor pds1p. Genes Dev. 10:3081-3093.
7. Funabiki, H., H. Yamano, K. Kumada, K. Nagao, T. Hunt, and M. Yanagida. 1996. Cut2 proteolysis required for sister-chromatid separation in fission yeast. Nature. 381:438-441.
8. Gorbsky, G.J., and W.A. Ricketts. 1993. Differential expression of a phosphoepitope at the kinetochores of moving chromosomes. J. Cell Biol. 122:1311-1321.
9. Hardwick, K.G., E. Weiss, F.C. Luca, M. Winey, and A.W. Murray. 1996. Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption. Science. 273:953-956.
10. He, X.W., T.E. Patterson, and S. Sazer. 1997. The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex. Proc. Natl. Acad. Sci. USA. 94:7965-7970.
11. Hershko, A., D. Ganoth, V. Sudakin, A. Dahan, L. Cohen, F. Luca, J.V. Ruderman, and E. Eytan. 1994. Components of a system that ligates cyclin to ubiquitin and their regulation by the protein kinase Cdc2. J. Biol. Chem. 269: 4940-4946.
12. Holloway, S.L., M. Glotzer, R.W. King, and A.W. Murray. 1993. Anaphase is initiated by proteolysis rather than by the inactivation of maturation promoting factor. Cell. 73:1393-1402.
13. Hoyt, M.A., L. Totis, and B.T. Roberts. 1991. S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell. 66:507-517.
14. Hwang, L.H., L.F. Lau, D.L. Smith, C.A. Mistrot, K.G. Hardwick, E.S. Hwang, A. Amon, and A.W. Murray. 1998. Budding yeast Cdc20: a target of the spindle checkpoint. Science. 279:1041-1044.
15. Irniger, S., S. Piatti, C. Michaelis, and K. Nasmyth. 1995. Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast. Cell. 81:269-277.
16. Kallio, M., J. Weinstein, J.R. Daum, D.J. Burke, and G.J. Gorbsky. 1998. Mammalian p55CDC mediates the association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase promoting complex and is involved in the regulation of anaphase onset and late mitotic events. J. Cell Biol. In press.
17. Kim, S.H., D.P. Lin, S. Matsumoto, A. Kitazono, and T. Matsumoto. 1998. Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint. Science. 279:1045-1047.
18. King, R.W., J.-M. Peters, S. Tugendreich, M. Rolfe, P. Hieter, and M.W. Kirschner. 1995. A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B. Cell. 81:279-288.
19. Li, R., and A.W. Murray. 1991. Feedback control of mitosis in budding yeast. Cell. 66:519-531.
20. Li, X., and R.B. Nicklas. 1995. Mitotic forces control a cell cycle checkpoint. Nature. 373:630-632.
21. Li, X.T., and R.B. Nicklas. 1997. Tension-sensitive kinetochore phosphorylation and the chromosome distribution checkpoint in praying mantid sperma-tocytes. J. Cell Sci. 110:537-545.
22. Li, Y., and R. Benezra. 1996. Identification of a human mitotic checkpoint gene hsMAD2. Science. 274:246-248.
23. Li, Y., C. Gorbea, D. Mahaffey, M. Rechsteiner, and R. Benezra. 1997. Mad2 associates with the cyclosome/anaphase-promoting complex and inhibits its activity. Proc. Natl. Acad. Sci. USA. 94:12431-12436.
24. Neff, M.W., and D.J. Burke. 1993. A delay in the Saccharomyces cerevisiae cell cycle that is induced by a dicentric chromosome and dependent upon mitotic checkpoints. Mol. Cell Biol. 12:3857-3864.
25. Nicklas, R.B., S.C. Ward, and G.J. Gorbsky. 1995. Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint. J. Cell Biol. 130:929-939.
26. Rieder, C.L., and S.P. Alexander. 1989. The attachment of chromosomes to the mitotic spindle and the production of aneuploidy in newt lung cells. Prog. Clin. Biol. Res. 318:185-194.
27. Rieder, C.L., A. Schultz, R. Cole, and G. Sluder. 1994. Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochore attachment to the spindle. J. Cell Biol. 127:1301-1310.
28. Rieder, C.L., R.W. Cole, A. Khodjakov, and G. Sluder. 1993. The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores. J. Cell Biol. 130:941-948.
29. Schott, E.J., and M.A. Hoyt. 1998. Dominant alleles of Saccharomyces cerevisiae CDC20 reveal its role in promoting anaphase. Genetics. 148:599-610.
30. Spencer, F., and P. Hieter. 1992. Centromere DNA mutations induce a mitotic delay in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 89:8908-8912.
31. Sudakin, V., D. Ganoth, A. Dahan, H. Heller, J. Hershko, F.C. Luca, J.V. Ruderman, and A. Hershko. 1995. The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end or mitosis. Mol. Biol. Cell. 6:185-197.
32. Taylor, S.S., and F. McKeon. 1997. Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to spindle damage. Cell. 89:727-735.
33. Tugendreich, S., J. Tomkiel, W. Earnshaw, and P. Hieter. 1995. CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. Cell. 81:261-268.
34. Wells, W.A.E., and A.W. Murray. 1996. Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast. J. Cell Biol. 133: 75-84.
35. Waters, J.C., R.-H. Chen, A.W. Murray, and E.D. Salmon. 1998. Mad2 and the spindle checkpoint: the roles of tension and microtubule attachment. J. Cell Biol. 141:1181-1191.
36. Yamada, H., K. Kumada, and M. Yanagida. 1997. Distinct subunit functions and cell cycle regulated phosphorylation of 20s APC/cyclosome required for anaphase in fission yeast. J. Cell Sci. 110:1793-1804.
37. Yamamoto, A., V. Guacci, and D. Koshland. 1996. Pds1p is required for faithful execution of anaphase in the yeast, Saccharomyces cerevisiae. J. Cell Biol. 133:85-97.
38. Zhang, D.H., and R.B. Nicklas. 1995. Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes. J. Cell Biol. 131:1125-1131.
39. Zhang, D.H., and R.B. Nicklas. 1996. Anaphase and cytokinesis in the absence of chromosomes. Nature. 382:466-468.
40. Zirkle, R.E. 1970. Ultraviolet-microbeum irradiation of newt-cell cytoplasm: spindle destruction, false antiphase, and delay of true anaphase. Rad. Res. 41: 516-537.