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Volumn 3, Issue 5, 1999, Pages 598-606

Initiation, elongation, and termination strategies in polyketide and polypeptide antibiotic biosynthesis

Author keywords

[No Author keywords available]

Indexed keywords

NATURAL PRODUCT; POLYKETIDE; POLYPEPTIDE; SYNTHETASE;

EID: 0032829915     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(99)00015-0     Document Type: Review
Times cited : (154)

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    • Characterization of the syringomycin synthetase gene cluster
    • The authors describe the synthetase of syringomycin, a lipodepsipeptide with phytotoxic properties. Syringomycin synthetase is unique in its architecture in that the 'colinearity rule' describing the assembly line process is not followed. There are nine monomers incorporated into syringomycin; the adenylation domain for threonine, the last amino acid in the chain, is not located in the last module upstream of the TE domain, where it would be expected, but rather on a separate protein, SyrB, located upstream of the main SyrE polypeptide. This implies that, at least, a specific in trans threonyl-AMP transfer must occur from the SyrB protein to the last PCP in SyrE
    • Guenzi E, Galli G, Grgurina I, Gross DC, Grandi G Characterization of the syringomycin synthetase gene cluster. J Biol Chem. 273:1998;32857-32863. The authors describe the synthetase of syringomycin, a lipodepsipeptide with phytotoxic properties. Syringomycin synthetase is unique in its architecture in that the 'colinearity rule' describing the assembly line process is not followed. There are nine monomers incorporated into syringomycin; the adenylation domain for threonine, the last amino acid in the chain, is not located in the last module upstream of the TE domain, where it would be expected, but rather on a separate protein, SyrB, located upstream of the main SyrE polypeptide. This implies that, at least, a specific in trans threonyl-AMP transfer must occur from the SyrB protein to the last PCP in SyrE.
    • (1998) J Biol Chem , vol.273 , pp. 32857-32863
    • Guenzi, E.1    Galli, G.2    Grgurina, I.3    Gross, D.C.4    Grandi, G.5


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