Reconstituting telomerase activity using the telomerase catalytic subunit prevents the telomere shorting and replicative senescence in human osteoblasts

Journal of Bone and Mineral Research

Volumn 16, Issue 8, 2001, Pages 1453-1464

  Yudoh, K ^a   Matsuno, H ^a   Nakazawa, F ^a   Katayama, R ^a   Kimura, T ^a  

^a UNIVERSITY OF TOYAMA   (Japan)

Author keywords

Osteoblast; Osteoporosis; Population doubling; Replicative senescence; Telomerase reverse transcriptase

Indexed keywords

TELOMERASE;

ADULT; AGED; APOPTOSIS; ARTICLE; BONE ATROPHY; BONE MARROW; CELL DIVISION; CELL STRAIN; CELL TRANSPLANTATION; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBUNIT; FEMALE; GENE THERAPY; HUMAN; HUMAN CELL; MALE; OSSIFICATION; OSTEOBLAST; OSTEOPENIA; SENESCENCE; STEM CELL; TELOMERE;

EID: 0034925471     PISSN: 08840431     EISSN: None     Source Type: Journal    
DOI: 10.1359/jbmr.2001.16.8.1453     Document Type: Article

References (62)

1. Replicative senescence: The human fibroblast comes of age
2. Replicative senescence: An old lives' tale?
3. Extension of life-span by introduction of telomerase into normal human cells
4. A biomarker that identifies senescent human cells in culture and in aging skin in vivo
5. Evidence for a relationship between longevity of mammalian species and life spans of normal fibroblasts in vitro and erythrocytes in vivo
6. Telomere length predicts replicative capacity of human fibroblast
7. Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes
8. Cell biology
9. Genetics of aging
10. Structure and function of telomeres
11. Telomeres shorten during aging of human fibroblasts
12. In vivo loss of telomeric repeats with age in humans
13. Telomere loss: Mitotic clock or genetic time bomb?
14. E6 of human papolomavirus type 16 can overcome the M1 stage of immortalization in human mammary epithelial cells but not in human fibroblasts
15. Telomere end-replication problem and cell aging
16. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats
17. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity
18. Specific association of human telomerase activity with immortal cells and cancer
19. Telomerase activity in normal leukocytes and in hematologic malignancies
20. Telomerase activity associated with acquisition of malignancy in human colorectal cancer
21. Telomeres, telomerase, and immortality
22. Telomerase catalytic subunit homologs from fission yeast and human
23. hEST2, the putative human telomerase catalytic subunit gene is up-regulated in tumor cells and during immortalization
24. Human telomerase contains evolutionarily conserved catalytic and structural subunits
25. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns indifferent cell types
26. Telomerase activation by hTRT in human normal fibroblasts and hepatocellular carcinoma
27. The RNA component of human telomerase
28. A mammalian telomerase-associated protein
29. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT
30. Transient expression of human telomerase extends the lifespan of normal human fibroblasts
31. The telomerase reverse transcriptase: Components and regulation
32. Mechanisms of disease: Bone marrow, cytokines, and bone remodeling. Emerging insights into the pathophysiology of osteoporosis
33. Increased osteoclast development after estrogen loss: Mediation by interleukin-6
34. Linkage of decreased bone mass with impaired osteoblastogenesis in a murine model of accelerated senescence
35. Evidence for a diminished maturation of preosteoblasts into osteoblasts during aging in rats: An ultrastructural analysis
36. The effect of aging on bone formation in porous hydroxyapatite: Biochemical and histological analysis
37. Age-related changes in osteogenic stem cells in mice
38. Bone-forming cells in clinical conditions
39. Relationship between bone formation rate and osteoblastic surface on different subdivisions of the endosteal envelope in aging and osteoporosis
40. Inhibition of osteoblastogenesis and promotion of apoptosis of osteoblasts and osteocytes by glucocorticoids: Potential mechanisms of their deleterious effects on bone
41. Influence of skeletal site of origin and donor age on osteoblastic cell growth and differentiation
42. Increased bone formation by prevention of osteoblast apoptosis with parathyroid hormone
43. Mitogenic responsiveness of human bone cells in vitro to hormones and growth factors decreases with age
44. A rapid quantitative assay for measuring alkaline phosphatase activity in osteoblastic cells in vitro
45. Differential regulation of plasminogen activator and plasminogen activator inhibitor by osteotropic factors in primary cultures of mature osteoblasts and osteoblast precursors
46. Growth of human embryonic fibroblasts at clonal density: Concordance with results from mass cultures
47. Relationship between donor age and the replicative lifespan of human cells in culture: A reevaluation
48. Signal transduction of mechanical stimuli is dependent on microfilament integrity: Identification of osteopontin as a mechanically induced gene in osteoblasts
49. Recruitment and proliferative responses of osteoblasts after mechanical loading in vivo determined using sustained-release bromodeoxyuridine
50. Primary human osteoblast proliferation and prostaglandin E2 release in response to mechanical strain in vitro
51. Parathyroid hormone modulates the response of osteoblast-like cells to mechanical stimulation
52. Osteoarthritis and related disorders: Pathology
53. Telomere shortening, telomerase expression, and chromosome instability in rat hepatic epithelial stem-like cells
54. Telomere maintenance in telomerase-deficient mouse embryonic stem cells: Characterization of an amplified telomeric DNA
55. Differentiation of immortal cells inhibits telomerase activity
56. TLP1: A gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family
57. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization
58. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span
59. Both Rb/p16 INK 4a inactivation and telomerase activity are required to immortalize human epithelial cells
60. Telomerase expression in human somatic cells does not induce charges associated with a transformed phenotype
61. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase