Extension of life-span by introduction of telomerase into normal human cells

Science

Volumn 279, Issue 5349, 1998, Pages 349-352

  Bodnar, Andrea G ^a   Ouellette, Michel ^b   Frolkis, Maria ^a   Holt, Shawn E ^b   Chiu, Choy Pik ^a   Morin, Gregg B ^a   Harley, Calvin B ^a   Shay, Jerry W ^b   Lichtsteiner, Serge ^a   Wright, Woodring E ^b  

^a GERON CORPORATION   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TELOMERASE;

ARTICLE; CELL CYCLE; CELL DIVISION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HUMAN; HUMAN CELL; LIFESPAN; MOLECULAR CLONING; PRIORITY JOURNAL; SENESCENCE; SKIN FIBROBLAST;

BETA-GALACTOSIDASE; BIOLOGICAL MARKERS; CATALYSIS; CELL AGING; CELL DIVISION; CELL LINE; CELL TRANSFORMATION, NEOPLASTIC; CLONING, MOLECULAR; DNA-BINDING PROTEINS; FIBROBLASTS; HOMEOSTASIS; HUMANS; KARYOTYPING; PHENOTYPE; PIGMENT EPITHELIUM OF EYE; PROTEINS; RNA; RNA-DIRECTED DNA POLYMERASE; STEM CELLS; TELOMERASE; TELOMERE; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0010045614     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5349.349     Document Type: Article

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66. We thank M. Liao, J.-F. Train, V. Tesmer, B. Frank, Y. Oei, S. Gitin, S. Wong, V. Votin, B. Lastelic, R. Adams, S. Amshey, D. Choi, and N. Spiff-Robinson for excellent technical assistance; W. Andrews and R. Adams for the construction of the MPSV vector; L. Hjelmeland for the RPE-340 cells; J. Smith for the BJ fibroblasts; M. Kozlowski for helpful discussions; L. Hayflick and T. R. Cech for critical reading of the manuscript; I. Savre-Train for the ribonuclease protection results; and W. O'Wheels for support during manuscript preparation. Supported in part by NIH grant AGO7992 (W.E.W.) and NIH postdoctoral fellowship AG05747 (S.E.H.).