Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span

Current Biology

Volumn 8, Issue 5, 1998, Pages 279-282

  Vaziri, Homayoun ^a   Benchimol, Samuel ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0032567967     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(98)70109-5     Document Type: Article

References (24)

1. Hayflick L, Moorhead P: The serial cultivation of human diploid strains. Exp Cell Res 1961, 25:585-621.
2. Harley CB, Futcher AB, Greider CW: Telomeres shorten during ageing of human fibroblasts. Nature 1990, 345:458-460.
3. Hastie ND, Dempster M, Dunlop MG, Thompson AM, Green DK, Allshire RC: Telomere reduction in human colorectal carcinoma and with ageing. Nature 1990, 346:866-868.
4. Vaziri H, Schachter F, Uchida I, Wei L, Zhu X, Effros R, et al.: Loss of telomeric DNAduring aging of normal and trisomy 21 human lymphocytes. Am J Hum Genet 1993, 52:661-667.
5. Greider CW, Blackburn EH: Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 1985, 43:405-413.
6. Counter CM, Avilion AA, LeFeuvre CE, Stewart NG, Greider CW, Harley CB, Bacchetti S: Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J 1992, 11:1921-1929.
7. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, et al.: Specific association of human telomerase activity with immortal cells and cancer. Science 1994, 266:2011-2015.
8. Morin GB: The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 1989, 59:521-529.
9. Olovnikov AM: A theory of marginotomy. Doklay Biochem 1971, 201:394-397.
10. Harley CB: Telomere loss: mitotic clock or genetic time bomb? Mutat Res 1991, 256:271-282.
11. Harley CB, Vaziri H, Counter CM, Allsopp RC: The telomere hypothesis of cellular aging. Exp Gerontol 1992, 27:375-382.
12. Lendvay TS, Morris DK, Sah J, Balas B, Lundblad V: Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics 1996, 144:1399-1412.
13. Counter CM, Meyerson M, Eaton EN, Weinberg RA: The catalytic subunit of yeast telomerase. Proc Natl Acad Sci USA 1997, 94:9202-9207.
14. Lingner J, Hughes TR, Shevchenko A, Mann M, Lundblad V, Cech TR: Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 1997, 276:561-567.
15. Nakamura TM, Morin GB, Chapman KB, Weinrich SL, Andrews WH, Lingner J, et al.: Telomerase catalytic subunit homologs from fission yeast and human. Science 1997, 277:955-959.
16. Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddie SD, et al.: hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 1997, 90:785-795.
17. Morgenstern JP, Land H: Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res 1990, 18:3587-3596.
18. Albritton LM, Tseng L, Scadden D, Cunningham JM: A putative murine ecotropic retrovirus receptor gene encodes a multiple membrane-spanning protein and confers susceptibility to virus infection. Cell 1992, 57:659-666.
19. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, et al.: A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci USA 1995, 92:9363-9367.
20. Weinrich SL, Pruzan R, Ma L, Ouellette M, Tesmer VM, Holt SE, et al.: Reconstitution of human telomerase with the template RNA component hTRand the catalytic protein subunit hTRT. Nat Genet 1997, 17:498-502.
21. Vaziri H, West MD, Allsopp RC, Davison TS, Wu YS, Arrowsmith CH, et al.: ATM-dependent telomere loss in aging human diploid fibroblasts and DNA damage lead to the posttranslational activation of p53 protein involving poly(ADP-ribose) polymerase. EMBO J 1997, 16:6018-6033.
22. Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu C-P, Morin GB, et al.: Extension of life-span by introduction of telomerase into normal human cells. Science 1998, 279:349-352.
23. Allsopp RC, Vaziri H, Patterson C, Goldstein S, Younglai EV, Futcher AB, et al.: Telomere length predicts replicative capacity of human fibroblasts. Proc Natl Acad Sci USA 1992, 89:10114-10118.
24. Kim NW, Wu F: Advances in quantitation and characterization of telomerase activity by telomeric repeat amplification protocol (TRAP). Nucleic Acids Res 1997, 25:2595-2597.