The transcriptional switch between the Drosophila genes Pig-1 and Sgs-4 depends on a SEBP1 binding site within a shared enhancer region

Molecular and General Genetics

Volumn 259, Issue 6, 1998, Pages 656-663

  Hofmann, A ^a   Lehmann, M ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

Drosophila; Enhancer promoter interactions; Pig 1; SEBP1; Sgs 4

Indexed keywords

DNA BINDING PROTEIN;

ANIMAL CELL; ARTICLE; DROSOPHILA; EMBRYO; ENHANCER REGION; GENE ACTIVATION; GENE TARGETING; GENETIC TRANSCRIPTION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SECRETION; SALIVARY GLAND; SILENCER GENE;

ANIMALS; BASE SEQUENCE; BINDING SITES; CELL NUCLEUS; CONSENSUS SEQUENCE; DNA FOOTPRINTING; DNA-BINDING PROTEINS; DROSOPHILA; DROSOPHILA PROTEINS; ENHANCER ELEMENTS (GENETICS); GENE EXPRESSION REGULATION; GENES, INSECT; GLUE PROTEINS, DROSOPHILA; INSECT PROTEINS; INTRONS; LARVA; MOLECULAR SEQUENCE DATA; REGULATORY SEQUENCES, NUCLEIC ACID; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSCRIPTION, GENETIC;

EID: 0031796020     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050860     Document Type: Article

References (39)

1. Ashburner M (1989) Drosophila: a laboratory handbook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
2. Bonner JJ, Parks C, Parker-Thornburg J, Mortin MA, Pelham HRB (1984) The use of promoter fusions in Drosophila genetics: isolation of mutations affecting the heat shock response. Cell 37:979-991
3. Bownes M (1994) The regulation of the yolk protein genes, a family of sex differentiation genes in Drosophila melanogaster. BioEssays 16:745-752
4. Chen C-N, Malone T, Beckendorf SK, Davis RL (1987) At least two genes reside within a large intron of the dunce gene of Drosophila. Nature 329:721-724
5. Crossley M, Orkin SH (1993) Regulation of the β-globin locus. Curr Opin Genet Dev 3:232-237
6. Crowley TE, Mathers PH, Meyerowitz EM (1984) A trims-acting regulatory product necessary for expression of the Drosophila melanogaster 68C glue gene cluster. Cell 39:149-156
7. Drapkin R, Merino A, Reinberg D (1993) Regulation of RNA polymerase II transcription. Curr Opin Cell Biol 5:469-476
8. Eissenberg JC, Elgin SCR (1991) Boundary functions in the control of gene expression. Trends Genet 7:335-340
9. Fletcher JC, Thummel CS (1995) The Drosophila E74 gene is required for the proper stage- and tissue-specific transcription of ecdysone-regulated genes at the onset of metamorphosis. Development 121:1411-1421
10. Guay PS, Guild GM (1991) The ecdysone-induced puffing cascade in Drosophila salivary glands: a Broad-Complex early gene regulates intermolt and late gene transcription. Genetics 129:169-175
11. Hatzopoulos AK, Schlokat U, Gruss P (1988) Enhancers and other cis-acting regulatory sequences. In: BD Hames, Glover DM (eds) Transcription and splicing. IRL Press, Oxford-Washington DC, pp 43-96
12. Hofmann A, Korge G (1987) Upstream sequences of dosage-compensated and non-compensated alleles of the larval secretion protein gene Sgs-4 in Drosophila. Chromosoma 96:1-7
13. Hofmann A, Keinhorst A, Krumm A, Korge G (1987) Regulatory sequences of the Sgs-4 gene of Drosophila melanogaster analysed by P-element mediated transformation. Chromosoma 96:8-17
14. Hofmann A, Garfinkel MD, Meyerowitz EM (1991) Cis-acting sequences required for expression of the divergently transcribed Drosophila melanogaster Sgs-7 and Sgs-8 glue protein genes. Mol Cell Biol 11:2971-2979
15. Karim FD, Guild GM, Thummel CS (1993) The Drosophila Broad-Complex plays a key role in controlling ecdysone-regulatcd gene expression at the onset of metamorphosis. Development 118:977-988
16. Koelle MR, Talbot WS, Segraves WA, Bender MT, Cherbas P, Hogness DS (1991) The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily. Cell 67:59-77
17. Korge G (1975) Chromosome puff activity and protein synthesis in larval salivary glands of Drosophila melanogaster. Proc Natl Acad Sci USA 72:4550-4554
18. Kunkel TA, Roberts JD, Zakour RA (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154:367-382
19. Landschulz WH, Johnson PF, Adashi EY, Graves BJ, McKnight SL (1988) Isolation of a recombinant copy of the gene encoding C/EBP. Genes Dev 2:786-800
20. Lehmann M (1996) Drosophila Sgs genes: stage and tissue specificity of hormone responsiveness. BioEssays 18:47-54
21. Lehmann M, Koolman J (1988) Ecdysteroid receptors of the blowfly Calliphora vicina: partial purification and characterization of ecdysteroid binding. Mol Cell Endocrinol 57:239-249
22. Lehmann M, Korge G (1995) Ecdysone regulation of the Drosophila Sgs-4 gene is mediated by the synergistic action of ecdysone receptor and SEBP 3. EMBO J 14:716-726
23. Lehmann M, Korge G (1996) The fork head product directly specifies the tissue-specific hormone responsiveness of the Drosophila Sgs-4 gene. EMBO J 15:4825-4834
24. Lehmann M, Wattler F, Korge G (1997) Two new regulatory elements controlling the Drosophila Sgs-3 gene are potential ecdysone receptor and fork head binding sites. Mech Dev 62: 15-27
25. Mach V, Ohno K, Kokubo H, Suzuki Y (1996) The Drosophila fork head factor directly controls larval salivary gland-specific expression of the glue protein gene Sgs-3. Nucleic Acids Res 24:2387-2394
26. McNabb SL, Beckendorf SK (1986) Cis-acting sequences which regulate expression of the Sgs-4 glue protein gene of Drosophila. EMBO J 5:2331-2340
27. Montell DJ, Rørth P, Spradling AC (1992) slow border cells, a locus required for a developmentally regulated cell migration during oogenesis, encodes Drosophila C/EBP. Cell 71:51-62
28. Mougneau E, von Seggern D, Fowler T, Rosenblatt J, Jongens T, Rogers B, Gietzen D, Beckendorf SK (1993) A transcriptional switch between the Pig-1 and Sgs-4 genes of Drosophila melanogaster. Mol Cell Biol 13:184-195
29. Ohlsson H, Edlund T (1986) Sequence-specific interactions of nuclear factors with the insulin gene enhancer. Cell 45:35-44
30. Pardue ML, Gall JG (1975) Nucleic acid hybridization to the DNA of cytological preparations. Methods Cell Biology 10:1-16
31. Rørth P, Montell DJ (1992) Drosophila C/EBP: a tissue-specific DNA-binding protein required for embryonic development. Genes Dev 6:2299-2311
32. Rubin GM, Spradling AC (1983) Vectors for P element-mediated gene transfer in Drosophila. Nucl Acids Res 11:6341-6351
33. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467
34. Shermoen AW, Jongens J, Barnett SW, Flynn K, Beckendorf SK (1987) Developmental regulation by an enhancer from the Sgs-4 gene of Drosophila. EMBO J 6:207-214
35. Spradling AC (1986) P element-mediated transformation. In: Roberts DB (ed) Drosophila: a practical approach. IRL Press, Oxford, UK, pp 175-197
36. Thomas HE, Stunnenberg HG, Stewart AF (1993) Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle. Nature 362:471-475
37. Vijay Raghavan K, Crosby MA, Mathers PH, Meyerowitz EM (1986) Sequences sufficient for correct regulation of Sgs-3 lie close to or within the gene. EMBO J 5:3321-3326
38. Weiher H, König M, Gruss P (1983) Multiple point mutations affecting the simian virus 40 enhancer. Science 219:626-631
39. Yao T-P, Segraves WA, Oro AE, McKeown M, Evans RM (1992) Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation. Cell 71:63-72